Preparation method of bacteriostatic hydrogel containing human umbilical cord mesenchymal stem cell freeze-dried powder

A stem cell, human umbilical cord technology, applied in bandages, medical science and other directions, can solve the problems of not promoting rapid wound healing, poor bacteriostatic effect, etc., achieving good water absorption and swelling performance, preventing infection, and promoting the effect of accelerating healing

Active Publication Date: 2021-07-27
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] However, the current existing hydrogel dressings all have disadvantages such as poor antibacterial effect and inability to promote rapid wound healing, which are not enough to provide an effective healing environment for wounds. Therefore, the development and application of ideal hydrogel dressings is still a huge challenge. , need to continue to explore in long-term research and clinical trials

Method used

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  • Preparation method of bacteriostatic hydrogel containing human umbilical cord mesenchymal stem cell freeze-dried powder
  • Preparation method of bacteriostatic hydrogel containing human umbilical cord mesenchymal stem cell freeze-dried powder
  • Preparation method of bacteriostatic hydrogel containing human umbilical cord mesenchymal stem cell freeze-dried powder

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preparation example Construction

[0033] A preparation method of the antibacterial hydrogel containing human umbilical cord mesenchymal stem cell freeze-dried powder of the present invention comprises the following steps:

[0034] Step 1: Human umbilical cord mesenchymal stem cells (human umbilical cords were provided by women who gave birth in Shanghai Dongfang Hospital, China. All subjects were informed and agreed. Human umbilical cord mesenchymal stem cells were isolated, cultured and certified) were cultured in MEM (Gibco, C12571500BT, China) containing 5% Ultra GRo-Advanced cell nutrient supplement (HeliosBioscience, HPCFDCGL50, USA), and incubated in an incubator at 37°C and 5% carbon dioxide. When the density of human umbilical cord mesenchymal stem cells reaches about 80%, wash 3 times with 0.9%wt% NaCl solution, culture in MEM medium without platelet lysis for 24h, collect the supernatant, vacuum at 0.02mbar and- Freeze at 90° C. for 48 hours to obtain freeze-dried powder of human umbilical cord mesen...

Embodiment 1

[0044] The preparation method of the antibacterial hydrogel of the present embodiment adopts following numerical preparation:

[0045] (1) Take by weighing 0.3g chitosan, be dissolved in the 3mL acetic acid solution of pH=4, obtain chitosan solution;

[0046] (2) Weigh 0.3 g of dialdehyde-derived ultrusan, and dissolve it in 6 mL of pure water to obtain a dialdehyde-derived ultrusan solution;

[0047] (3) Weigh 0.15g of dopamine hydrochloride and dissolve it in 3mL of pure water to obtain dopamine hydrochloride solution;

[0048] (4) above-mentioned three kinds of solutions are mixed and stirred overnight at room temperature, obtain bisaldehyde ulvasan / chitosan / dopamine hydrogel;

[0049] (5) Weigh 0.3997g (2mM) of silver nitrate solid, dissolve it in 50mL of pure water to obtain a 0.04M silver nitrate solution, and soak the above-mentioned dialdehyde-formed ulvato / chitosan / dopamine hydrogel In the prepared silver nitrate solution, react in a dark environment for 20 minutes ...

Embodiment 2

[0052] The preparation method of the antibacterial hydrogel of the present embodiment adopts following numerical preparation:

[0053] (1) Take by weighing 0.2g chitosan, be dissolved in the 3mL acetic acid solution of pH=5, obtain chitosan solution;

[0054] (2) Weigh 0.3 g of dialdehyde-derived ultrusan, and dissolve it in 6 mL of pure water to obtain a dialdehyde-derived ultrusan solution;

[0055] (3) Weigh 0.15g of dopamine hydrochloride and dissolve it in 3mL of pure water to obtain dopamine hydrochloride solution;

[0056] (4) above-mentioned three kinds of solutions are mixed and stirred overnight at room temperature, obtain bisaldehyde ulvasan / chitosan / dopamine hydrogel;

[0057] (5) Weigh 0.7994g (4mM) of silver nitrate solid and dissolve it in 50mL of pure water to obtain a 0.08M silver nitrate solution. Soak the above-mentioned dialdehyde-derived ultrusan / chitosan / dopamine hydrogel in the configured silver nitrate solution, and react in a dark environment for 20 ...

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Abstract

The invention relates to a preparation method of bacteriostatic hydrogel containing human umbilical cord mesenchymal stem cell freeze-dried powder. The preparation method comprises the following steps: firstly, culturing and incubating human umbilical cord mesenchymal stem cells, collecting supernate of the human umbilical cord mesenchymal stem cells, and freeze-drying to obtain stem cell freeze-dried powder; freeze-drying ulva powder, extracting ulva from the freeze-dried ulva powder, oxidizing to obtain dialdehyde ulva, carrying out Schiff base reaction on chitosan and dialdehyde ulva, introducing dopamine, preparing silver nanoparticles, and constructing to obtain the dialdehyde ulva/chitosan/dopamine/silver nanoparticle composite hydrogel; and finally, dissolving the human umbilical cord mesenchymal stem cell freeze-dried powder in a phosphate buffer solution to obtain a stem cell freeze-dried powder solution, and the dialdehyde ulva/chitosan/dopamine/silver nanoparticle composite hydrogel is soaked for a certain time. The composite hydrogel system disclosed by the invention can inhibit bacteria, prevent wound infection and promote accelerated healing of wounds; and the water-absorbing swelling property and the moisture retention property are good.

Description

[0001] 1. Technical field: [0002] The invention relates to the technical field of hydrogel preparation, in particular to a preparation method of an antibacterial hydrogel containing human umbilical cord mesenchymal stem cell freeze-dried powder. [0003] 2. Background technology: [0004] Human skin is the first natural barrier with the functions of resisting the invasion of external microorganisms, avoiding the loss of body fluids and maintaining the stability of the internal environment of the body. However, after the skin suffers severe injuries such as surgery, burns, and skin diseases, this layer of barrier will lose its protective effect, eventually forming a wound, and it is easy to be invaded by external microorganisms such as Escherichia coli and Staphylococcus aureus and cause infection, making the wound If it cannot heal for a long time, it may cause many serious complications, such as sepsis, tetanus, and persistent high fever, etc., seriously affecting the qualit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/075C08L5/00C08L5/08A61L26/00
CPCC08J3/075A61L26/008A61L26/0057A61L26/0095A61L26/0004A61L26/0066C08J2305/00C08J2405/08A61L2300/104A61L2300/412A61L2300/404A61L2300/236A61L2300/30C08L5/08C08L5/00Y02A50/30
Inventor 涂琴任宇艾力.艾尔肯王进义华进联李雯黄婷袁茂森李天保许娟
Owner NORTHWEST A & F UNIV
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