A kind of method for promoting the germination of Chunlan seeds

A technology of seeds and Chunlan, applied in the field of botany, can solve problems such as poor practicability, difficulties, and temperature rise of the seed embryo, and achieve the effects of improving impermeability, improving non-hydrophilicity, and promoting seed germination

Active Publication Date: 2022-05-24
JIANGSU LIXIAHE REGION AGRI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the physical method, it is very difficult to cut the seed coat with ophthalmic scissors or surgical blades because the seeds are very small, and the operability is not strong, so it cannot be widely used; the seed coat is broken by ultrasonic vibration, not only the seed coat is not easy to break, but it is easy to make the seed coat on the contrary. The temperature of the embryo part rises, the embryo tissue is damaged, and the seeds are browned
In the chemical method, using NaOH solution and NaCIO solution to corrode the seed coat has a certain effect, but NaOH solution and NaCIO solution have certain damage to the seed embryo. In order to remove NaOH solution and NaCIO solution, it must be washed several times with sterile water. Filtration, difficult to operate, not very practical

Method used

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  • A kind of method for promoting the germination of Chunlan seeds
  • A kind of method for promoting the germination of Chunlan seeds
  • A kind of method for promoting the germination of Chunlan seeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] 1. Cultivation of capsules:

[0047] Using the spring orchid variety "Cui Gaihe" as the female parent and the Chunlan variety "Global Heding" as the male parent, artificial hybridization was carried out to cultivate the capsules. After the spring orchid variety "Cui Gai He" bloomed for 5 days, remove the nose and pollen blocks, and use a small white gauze bag to cover the style to avoid insect pollination. The pollen block of "Universal Lotus Ding" is placed in the column cavity under the nose. After pollination, the style is covered with a small white gauze to prevent repeated pollination by insects and ensure the success rate of hybridization. The small gauze bag is removed after the seeds are fertilized and expanded, and normal cultivation is carried out, and the capsule is continuously expanded to obtain a capsule.

[0048] 2. Disinfection:

[0049]Step 1. Fold off the capsule, peel off the remaining petals, wipe the surface of the capsule with a mass fraction of ...

Embodiment 2

[0052] S1 configures nutrient solution and culture solution, wherein the nutrient solution includes 0.3mg / L 1 / 2MS+NAA medium, 20g / L sucrose and 0.5g / L peptone, and the pH of the nutrient solution is 5.4; the culture solution includes 0.3mg / L 1 / 2MS+NAA medium, 20g / L sucrose, 0.5g / L activated carbon and 0.5g / L peptone, the pH of the culture medium is 5.4;

[0053] S2 get 0.1g of the seeds obtained in Example 1 and add it into a 150mL sterile syringe, use the syringe to inhale 100mL of nutrient solution and 0.02mL of Tween 80, shake horizontally for 3min, and leave it to stand for 12h vertically after shaking to obtain treatment solution A;

[0054] S3 derives the upper layer of treatment solution A from the syringe mouth, and then puts a sterile syringe filter on the syringe mouth to filter out other treatment solutions to obtain seeds, add 100 mL of culture solution to the syringe, shake horizontally for 1 min, and obtain treatment solution B ;

[0055] S4, put the needle cap ...

Embodiment 3

[0058] S1 configures nutrient solution and culture solution, wherein the nutrient solution includes 0.2mg / L 1 / 2MS+NAA medium, 25g / L sucrose and 0.6g / L peptone, the pH of the nutrient solution is 5.1; the culture solution includes 0.2mg / L 1 / 2MS+NAA medium, 25g / L sucrose, 0.4g / L activated carbon and 0.6g / L peptone, the pH of the culture medium is 5.1;

[0059] S2 get 0.3g of the seeds obtained in Example 1 and add it to a 150mL sterile syringe, use the syringe to inhale 100mL of nutrient solution and 0.03mL of Tween 80, shake horizontally for 3min, and leave it to stand for 12h vertically after shaking to obtain treatment solution A;

[0060] S3 derives the upper layer of treatment solution A from the syringe mouth, and then puts a sterile syringe filter on the syringe mouth to filter out other treatment solutions to obtain seeds, add 100 mL of culture solution to the syringe, shake horizontally for 1 min, and obtain treatment solution B ;

[0061] S4, put the needle cap on the...

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Abstract

The invention discloses a method for promoting the germination of Chunlan seeds, which comprises the following steps: disinfecting the Chunlan capsules; placing the seeds in a sealed sterilization container, adding nutrient solution and Tween 80, shaking horizontally, and statically vertically after the shaking is completed. place to obtain treatment solution A; take out the seeds from treatment solution A, place the seeds in a sealed sterilized container, add culture medium, shake horizontally, and obtain treatment solution B; perform negative pressure treatment on treatment solution B, and discharge the seed coat bubbles to obtain the treatment solution C; pour the treatment solution C into a culture bottle for seed cultivation. The present invention uses Tween 80 and nutrient solution to treat the seed coat, Tween 80 can effectively improve the non-hydrophilicity of the testa, increase the contact between the nutrient solution and the testa, and improve the impermeability of the testa; then place the seeds In the culture medium, negative pressure treatment is carried out to discharge the bubbles of the seed coat, and the culture medium enters the seed coat at the same time to fully contact the embryo cells, effectively promoting seed germination.

Description

technical field [0001] The invention relates to the technical field of botany, in particular to a method for promoting the germination of Chunlan seeds. Background technique [0002] Chunlan is a perennial herb of the Orchidaceae, Orchid genus, and Terrestrial Orchid. It is a representative species of Chinese orchid. Spring orchid has beautiful leaves, elegant plant shape, quiet flower fragrance and rich petal shape. The natural flowering period is around the Spring Festival. Compared with other orchids that are not resistant to cold, Chunlan has obvious advantages as a high-end potted flower and New Year's Eve flower. The development potential of Chunlan industry is huge, but my country's Chunlan has traditionally formed the habit of focusing on varieties and light-scale breeding and production. Its value orientation is also based on the degree of rarity. There are almost no varieties suitable for ordinary consumers, and the output and commercialization are low. , unable to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01C1/00A01C1/08
CPCA01C1/00A01C1/08
Inventor 孙叶刘春贵魏小羽李风童马辉单东方弓剑陶凡
Owner JIANGSU LIXIAHE REGION AGRI RES INST
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