Method for quantitatively detecting iodine content in whole blood, urine or milk

A technology for quantitative detection and iodine content, applied in the detection field, can solve the problems of complex pretreatment, complex operation, and many manual steps, and achieve the effects of high sensitivity, low background signal, and wide linear range

Pending Publication Date: 2021-07-23
杭州凯莱谱精准医疗检测技术有限公司
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Problems solved by technology

The instrument used in this method has strict requirements on the experimental conditions, the pretreatment is complex and the highly toxic trivalent arsenic compound is used, the method has many manual steps, and the operation is complicated, so it is not suitable for the detection of large clinical samples.

Method used

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  • Method for quantitatively detecting iodine content in whole blood, urine or milk
  • Method for quantitatively detecting iodine content in whole blood, urine or milk
  • Method for quantitatively detecting iodine content in whole blood, urine or milk

Examples

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Embodiment 1

[0039] 1: Standard product preparation: Purchasing iodine single element standard solution (1mg / mL) and tellurium element standard solution (1mg / mL) as internal standard substances. Then dilute the standard solution with 1% ammonia diluent, and prepare the standard working curve according to the concentration in the table below.

[0040] iodine STD1 STD2 STD3 STD4 STD5 STD6 STD7 STD8 μg / L 10 25 50 200 500 1000 2500 5000

[0041] 2. Sample processing:

[0042] Mix the urine, whole blood or milk samples thoroughly with a mixer at room temperature. Sample pretreatment: Take 200μL sample (mix well before sampling to suspend all sediments), add 2.0mL (500mL ultrapure water, 5mL ammonia water and 0.1mL 1mg / mL tellurium standard solution) diluent, mix well .

[0043] (3) Take 4mL centrifuge tubes, mark SD1 to SD8, and several samples, mark Sample1, 2..., add 2.0mL internal standard diluent to each centrifuge tube, then add 200μL standard ...

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Abstract

The invention relates to a method for quantitatively detecting an iodine content in whole blood, urine or milk. The method is characterized by comprising the following steps: (1) processing a sample: sufficiently and uniformly mixing urine, whole blood or milk samples by using a mixer at a room temperature; taking a certain amount of sample, adding 8-12 times volume of diluent, and uniformly mixing; and (2) detecting on a machine: washing the needle with a 0.5-1.5% ammonia water solution, wherein the liquid forms aerosol in an atomizer filled with a gas. The method disclosed by the invention eliminates a matrix effect, realizes automatic detection, is high in detection speed, and is suitable for quantitative analysis of the iodine content in various sample types.

Description

technical field [0001] The invention relates to the technical field of detection, and mainly relates to a method for quantitatively detecting iodine content in whole blood, urine or milk. Background technique [0002] The detection methods of iodine mainly include arsenic-cerium catalytic spectrophotometry, gas chromatography, inductively coupled plasma mass spectrometry and so on. Pretreatment methods mainly include wet digestion, dry ashing and microwave digestion. [0003] Arsenic-cerium catalytic spectrophotometry is a standard method for urine iodine analysis (WS / T107-1999), which is an analysis method established based on the selective absorption of light by substances. This method has high sensitivity and accurate results, but requires strict experimental conditions. For example, urine samples need to be digested with ammonium persulfate, which takes a long time, and the arsenic trioxide in the reagent is highly toxic, which is easy to pollute the environment and ca...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N1/38G01N27/62
CPCG01N1/28G01N1/38G01N27/62
Inventor 孔子青马金飞杨楠江玉祥刘华芬
Owner 杭州凯莱谱精准医疗检测技术有限公司
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