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Orthopedic material surface coating for promoting osteogenesis and immunoregulation and preparation method thereof

An immunomodulatory and surface coating technology, which is applied in coatings, pharmaceutical formulations, medical science, etc., can solve the problems of molecular leakage, universal applicability, and practicability discounts, and achieve stable reactions, simple and easy preparation steps, and promote and immunomodulatory effects

Active Publication Date: 2021-07-23
THE FIRST AFFILIATED HOSPITAL OF SOOCHOW UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current physical adsorption method will cause serious molecular leakage, while the chemical method requires complex chemical means and non-biocompatible chemical molecules to serve as bridges for active molecules.
Therefore, the general applicability and practicality of this type of method are greatly reduced

Method used

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  • Orthopedic material surface coating for promoting osteogenesis and immunoregulation and preparation method thereof
  • Orthopedic material surface coating for promoting osteogenesis and immunoregulation and preparation method thereof
  • Orthopedic material surface coating for promoting osteogenesis and immunoregulation and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Preparation of Mussel-derived Bioactive Peptide (DBCO-DOPA Polypeptide) Adhering to the Surface of Various Solid Materials

[0030] Based on the standard fluorenylmethyl (Fmoc) solid-phase peptide synthesis strategy, DOPA units were introduced into the sequence using acetonide-protected Fmoc-DOPA (acetone)-OH, and the resulting peptides were capped with acetyl groups at N end, to obtain polymer DBCO-DOPA ((Ac-(DOPA)-G-(DOPA)-K(PEG5-Azido)-(DOPA)-G-(DOPA) ) ( figure 1 ), referred to as DBCO-DOPA polypeptide.

[0031] The synthesized DBCO-DOPA polypeptide was dissolved in dimethyl sulfoxide (Dimethyl sulfoxide, DMSO) at 1 mg / 10 μL, and diluted to 0.01 mg / mL with sterile deionized water when performing experiments.

[0032] Different solid materials were selected as the adhesion objects of DBCO-DOPA peptides, including stainless steel sheets (StainlessSteel, SS), titanium sheets (Ti), polystyrene sheets (TCPs), glass sheets (Glass) and polyether ether ketone (P...

Embodiment 2

[0035] Example 2 The role of bone morphogenetic protein 2 active peptide (referred to as BMP2p active peptide) in promoting bone formation and immune regulation

[0036] Biosynthetic BMP2p active peptide, its sequence is KIPKASSVPTELSAISTLYL, and the molecular formula of BMP2p active peptide is as follows Figure 5 As shown in A.

[0037] Firstly, the effect of BMP2p active peptide on the osteogenic differentiation of mesenchymal stem cells pretreated with titanium particles was studied. Pretreat UMSCs cells with titanium particles, the specific steps are as follows: the UMSCs cells were 1 × 10 per well 5 Inoculate a 6-well plate at a density of one cell, and replace the complete medium containing titanium particles at a concentration of 0.1 mg / mL under the condition that 30% of the bottom surface is covered. The complete medium without titanium particles was replaced every two days until 6 days later, when cells and titanium particles were trypsinized. Centrifuge the mixtu...

Embodiment 3

[0039] Example 3 BMP2p active peptide is colonized on the surface of PEEK material coated with DBCO-DOPA polypeptide through click chemistry and exerts the functions of promoting osteogenesis and immune regulation

[0040] The diameter of the cast-molded medical-grade PEEK disc is 15.5 mm. Before the surface modification of the DBCO-DOPA polypeptide coating, all materials were soaked in acetone for 24 hours overnight. The next day, it was first soaked in acetone at room temperature for 10 minutes, then in an ultrasonic bath for 3 minutes, then soaked in absolute ethanol for 10 minutes, air-dried, and then treated in an ultrasonic bath for 3 minutes. It was washed 3 times and placed in a 10 cm petri dish. Finally, each sample was cleaned by plasma for 2 min / time under vacuum conditions, and cleaned twice to promote the grafting ability of the surface. The radio frequency is adjusted to 40% of the maximum power, 80W, and the internal pressure is 99.3KPa.

[0041] The materials ...

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Abstract

The invention discloses an orthopaedic material surface coating for promoting osteogenesis and immunoregulation and a preparation method thereof. The coating is attached to the surface of an orthopaedic material through a medium by active peptides of a bone morphogenetic protein 2 , the amino acid sequence of the active peptides of the bone morphogenetic protein 2 is KIPKASSVPTELSAISTLYL, and the medium is a peptide sequence which includes a plurality of 3,4-dihydroxyphenylalanine DOPA. The preparation steps of the coating are simple and feasible, large equipment is not needed, the reaction is stable, and the environment will not be polluted. Experimental results show that compared with a coating prepared through a traditional material surface modification method, the material surface coating prepared through the preparation method has the functions of promoting osteogenesis and immunoregulation, and a new way is provided for further application of orthopedic materials to the fields of trauma and scaffold for tissue engineering.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a surface coating of an orthopedic material promoting bone formation and immune regulation and a preparation method thereof. Background technique [0002] As the number of patients suffering from bone and joint diseases and injuries is increasing year by year, the demand for orthopedic implants is also increasing. Clinically, the osteogenic properties of existing implants are still unsatisfactory, and implant particles (such as titanium or ceramic particles) will promote the polarization of macrophages to M1 after being phagocytized by macrophages in vivo , causing the release of a series of inflammatory mediators affecting osteoblast bone formation and promoting osteoclast bone resorption, which in turn affects the stability of the implant. [0003] Bone morphogenetic protein-2 (BMP-2) is a commonly used clinical osteogenic factor, which can activate mesenchymal ...

Claims

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Application Information

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IPC IPC(8): A61L27/34A61L27/54A61L27/22
CPCA61L27/34A61L27/54A61L27/227A61L2300/426A61L2300/412A61L2300/606A61L2400/18A61L2430/02C08L89/00
Inventor 施勤潘国庆周熙超赵环
Owner THE FIRST AFFILIATED HOSPITAL OF SOOCHOW UNIV
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