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Method for establishing pulmonary edema rabbit animal model

An animal model and a technology for establishing a method are applied in the field of establishing a rabbit animal model of pulmonary edema, which can solve problems such as affecting cardiac function, increasing myocardial oxygen consumption, and unfavorable observation of simple pulmonary edema, so as to reduce the impact and ensure the carrying capacity. Effect

Active Publication Date: 2021-07-23
刘敬
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Problems solved by technology

[0003] In addition, epinephrine injection will affect heart function, causing increased heart rate, increased myocardial oxygen consumption, etc., which is not conducive to the effect of simple pulmonary edema on heart function Observed

Method used

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  • Method for establishing pulmonary edema rabbit animal model
  • Method for establishing pulmonary edema rabbit animal model
  • Method for establishing pulmonary edema rabbit animal model

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Experimental program
Comparison scheme
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Embodiment Construction

[0045] The following is further described in detail through specific implementation methods:

[0046] The establishment method of the pulmonary edema rabbit animal model is mainly to externally and intermittently infuse the experimental animals with drugs that change the increase of lung water content, so as to increase the liquid content in the lung interstitium, alveoli and cells, thereby inducing the model to produce pulmonary edema lesions. The detailed construction steps are as follows:

[0047] 1 Materials and methods

[0048] 1.1 Experimental animals

[0049] Six male New Zealand rabbits were purchased from Beijing Keyu Animal Breeding Center, animal quality license number: SCXK (Beijing) 2017-0002. The weight ranged from 2.8 to 3.1kg, with an average of 2.9±0.1kg.

[0050] 1.2 Main experimental reagents and instruments

[0051] Pentobarbital sodium, 0.9% sodium chloride solution, heparin sodium injection, rabbit table, animal hair remover, vascular forceps, scalpel...

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Abstract

The invention discloses a method for establishing a pulmonary edema rabbit animal model, belongs to the field of biological model construction, and aims to overcome the defect that in the prior art, complicating respiratory system symptoms are generated by reducing heart rate or other external circulation influences. In order to overcome the defects, according to the technical scheme, a medicament for changing the increase of the lung water content is externally and discontinuously injected into an animal to be experimented, so that the liquid content in pulmonary interstitial substances, pulmonary alveoli and cells is increased, and the model is induced to generate pulmonary edema lesion. Compared with the prior art of an oleic acid injection method, an epinephrine instillation method and a chloroform injection method, in the technical scheme, the medicament is directly perfused into the lung to change the concentration of lung water, so that mutagenesis is realized, the influence of heart rate or other external circulation is avoided, mutagenesis models with complicating respiratory system symptoms are overcome, meanwhile, active substances on the surface of the lung are reserved to the maximum extent (direct internal injection), and the influence of complications on experimental data is reduced.

Description

technical field [0001] The invention belongs to the field of biological model construction, in particular to a method for establishing a pulmonary edema rabbit animal model. Background technique [0002] At present, there are many domestic methods for replicating animal models of experimental pulmonary edema / EVLW increase, among which the most commonly used methods are oleic acid injection, epinephrine instillation and chloroform injection, and there are also injections of fibrinogen and thrombin through the cisterna magna. Established a rabbit model of neurogenic pulmonary edema. In the pulmonary edema model established by these methods, in addition to the pathological changes of pulmonary edema, there are often pathological changes such as pulmonary congestion and pulmonary hemorrhage, and such pathological changes appear as different ultrasonic signs under lung ultrasound, which will affect the use of pulmonary edema. Ultrasound assessment of lung water content. [0003...

Claims

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Application Information

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IPC IPC(8): A61D7/00A61D1/00G01N21/84G01N5/04G01N1/28G01N1/30
CPCA61D7/00A61D1/00G01N21/84G01N5/045G01N1/286G01N1/30
Inventor 郭果宗海峰刘敬
Owner 刘敬
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