Method for prolonging fresh preservation period of chilled fresh meat by using bacteriostatic lactic acid bacteria composite fresh-keeping solution
A technology for cold fresh meat, freshness period, applied in the direction of preserving meat/fish with chemicals, preserving meat/fish with protective coating, bacteria used in food preparation, etc.
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Embodiment 1
[0049] A method for extending the fresh-keeping period of chilled fresh meat by utilizing bacteriostatic lactic acid bacteria, comprising the steps of:
[0050] (1) Processing of meat
[0051] Sterilize the chopping boards and knives in a sterilizing pot, and wipe them with disinfectant alcohol before use. Under the aseptic condition of the aseptic table, remove the excess fascia and fat from the fresh meat slaughtered in the meat joint factory on the same day, and cut it into meat pieces weighing about 100-1000g for use;
[0052] (2) Expanded culture of Lactobacillus
[0053] Aseptically inoculate Lactobacillus plantarum into liquid MRS medium and culture it in a 37°C incubator for about 12 hours to fully activate it; then inoculate the activated strains into a large cone filled with liquid MRS medium In the bottle, expand in a shaker at a constant temperature of 37°C for about 12 hours, and set aside;
[0054] (3) Collection of Lactobacillus cells
[0055] Pour the cultu...
Embodiment 2
[0066] With above-mentioned embodiment 1, its difference is:
[0067] Step (5) in the fresh-keeping solution: spray the Lactobacillus plantarum suspension on the meat sample at a volume fraction of 1.5% (V / W%), let stand for 1min, drain and set aside;
Embodiment 3
[0069] With above-mentioned embodiment 1, its difference is:
[0070] Step (5) in the fresh-keeping solution: spray the Lactobacillus plantarum suspension on the meat sample at a volume fraction of 2.0% (V / W%), let stand for 1min, drain and set aside;
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