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A method for extracting and isolating mogrosinic acid from the roots of grosvenoria grosvenori

A technique of Luo Han Guo and Luo Han Guo, which is applied in the field of extraction and separation of natural triterpenoid acids, can solve problems such as no literature disclosure, and achieve the effects of process coherence, material and energy saving, and low production cost.

Active Publication Date: 2022-04-19
HUNAN HUACHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] At present, there is no document disclosing the method of industrially obtaining the total acid of Luo Han Guo from the root of Luo Han Guo

Method used

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  • A method for extracting and isolating mogrosinic acid from the roots of grosvenoria grosvenori
  • A method for extracting and isolating mogrosinic acid from the roots of grosvenoria grosvenori

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] (1) Acidic alcohol extraction: take 10kg of dried Luo Han Guo root, grind it to a particle size of 2-3mm, add 180L of acidic methanol, the concentration of methanol is 70% by volume, which contains 0.5% hydrochloric acid, then add 70g of sodium sulfite and 6g of plant Acid, reflux extraction for 4.5 hours, filter to obtain acidic alcohol extract;

[0048] (2) Alkali adjustment, ceramic membrane filtration: the acidic alcohol extract is cooled to room temperature, the pH value is adjusted to 12 with aqueous sodium hydroxide solution, and the pore diameter is 50nm ceramic membrane filtration, and the filtration pressure is 0.4Mpa to obtain the ceramic membrane filtrate;

[0049] (3) concentration under reduced pressure: the ceramic membrane filtrate is concentrated under reduced pressure to no alcohol to obtain a concentrated solution;

[0050] (4) The first extraction and removal of impurities: the concentrated solution was extracted 3 times with 80L petroleum ether, all...

Embodiment 2

[0056] (1) Acidic alcohol extraction: take 10kg of dried Luo Han Guo root, grind it to a particle size of 2-3mm, add 150L of acidic ethanol, the concentration of ethanol is 75% by volume, which contains 0.8% hydrochloric acid, then add 100g of sodium sulfite and 8g of plant Acid, and reflux extraction for 5 hours, filtered to obtain an acidic alcohol extract;

[0057] (2) Alkali adjustment, ceramic membrane filtration: the acidic alcohol extract is cooled to room temperature, the pH value is adjusted to 11 with aqueous sodium hydroxide solution, and the pore diameter is 100nm ceramic membrane filtration, and the filtration pressure is 0.3Mpa to obtain the ceramic membrane filtrate;

[0058] (3) concentration under reduced pressure: the ceramic membrane filtrate is concentrated under reduced pressure to no alcohol to obtain a concentrated solution;

[0059] (4) The first extraction and removal of impurities: extract the concentrated solution twice with 100L 6# solvent oil, let ...

Embodiment 3

[0065] (1) Acidic alcohol extraction: Take 10kg of dried Luo Han Guo root, grind it to a particle size of 2-3mm, add 120L of acidic methanol, the concentration of methanol is 80% by volume, which contains 1% hydrochloric acid, then add 50g of sodium sulfite and 5g of plant Acid, reflux extraction for 4 hours, filter to obtain acidic alcohol extract;

[0066] (2) Alkali adjustment, ceramic membrane filtration: the acidic alcohol extract is cooled to room temperature, the pH value is adjusted to 12 with potassium hydroxide aqueous solution, and the pore diameter is 50nm ceramic membrane filtration, and the filtration pressure is 0.4Mpa to obtain the ceramic membrane filtrate;

[0067] (3) concentration under reduced pressure: the ceramic membrane filtrate is concentrated under reduced pressure to no alcohol to obtain a concentrated solution;

[0068] (4) The first extraction and removal of impurities: the concentrated solution was extracted 3 times with 90L cyclohexane, allowed ...

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Abstract

The invention relates to a method for extracting and separating mogrosanthic acid from the root of Luo Han Guo, which comprises the following steps: the root of Luo Han Guo is reflux extracted with an acidic alcohol solution, the extract is adjusted to be alkaline, filtered, concentrated, and the first step is carried out with a low-polarity organic solvent. First extraction, layered into an alkaline water layer and an organic solvent layer, take the alkaline aqueous layer to adjust to acidity, and use a low-polarity organic solvent for the second extraction, layered into an acidic aqueous layer and an organic solvent layer, and the organic solvent layer for Decolorize, filter, concentrate, and dry to obtain the total acid product of Luo Han Guo. The present invention adopts the synchronous method of "reflux extraction-hydrolysis", realizes the extraction of mogrosin and the hydrolysis of mogroside in the same step, saves materials and energy, and improves the yield of mogrosin. The method of the invention is coherent and simple in process, strong in operability, low in production cost, high in product content and high in yield, and is suitable for industrial production for extracting and isolating mogroscarpic acid from roots of grosvenoria grosvenori.

Description

technical field [0001] The invention relates to a method for extracting and separating natural triterpene acids, in particular to a method for extracting and separating mogroscarpic acid from the root of Luo Han Guo. Background technique [0002] Luo Han Guo is a rare local specialty in Guilin, mainly produced in Yongfu County and Longsheng County, and it is also one of the first batch of "dual-use medicine and food" materials approved by the state. Mogroside is a unique natural high-power sweetener in Luo Han Guo. Its sweetness is 300 times that of sucrose, and its calories are zero. preventive effect. As a food, mogroside is safe and non-toxic. According to the national mandatory standard "GB2760 Food Additive Use Standard", mogroside can be used in various foods without limit. [0003] In the current production practice, the industrial production and utilization of Luo Han Guo mainly focuses on the extraction and separation of sweet glycosides in the fruit of Luo Han Gu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J71/00C07J9/00
CPCC07J71/0005C07J9/005
Inventor 黄俊李伟黄华学贺进军宋谷良江小龙
Owner HUNAN HUACHENG BIOTECH
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