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Method for detecting content of DHEA in human body fluid

A technology for detecting human body and content, applied in the field of detection, can solve problems such as inaccuracy of DHEA, and achieve the effects of simple pretreatment process, high sensitivity and strong specificity

Pending Publication Date: 2021-07-02
艾可泰科(浙江)控股有限公司
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Problems solved by technology

[0005] The purpose of the present invention is to solve the inaccurate problem of detecting DHEA in saliva in the prior art, and propose a method for detecting the content of DHEA in human body fluid

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  • Method for detecting content of DHEA in human body fluid
  • Method for detecting content of DHEA in human body fluid
  • Method for detecting content of DHEA in human body fluid

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Embodiment Construction

[0040] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention.

[0041] refer to Figure 1-2 , a method for detecting the content of DHEA in human body fluids, including the detection of DHEA in saliva by high performance liquid chromatography tandem mass spectrometry:

[0042] S1. Add the following reagents to the kit, mobile phase 1, mobile phase 2, standard mother solution, internal standard solution, diluent, extract, quality control product and reconstitution solvent;

[0043] S2. Using high performance liquid chromatography tandem mass spectrometry to detect dehydroepiandrosterone in the pretreated saliva;

[0044] S3, using high performance liquid chromatography to separate dehydroepiandrosterone from interfering substance...

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Abstract

The invention discloses a method for detecting the content of DHEA in human body fluid. The method comprises the steps of detecting dehydroepiandrosterone in saliva by using a high performance liquid chromatography-tandem mass spectrometry technology, specifically, S1, adding the following reagents into a kit: a mobile phase I, a mobile phase II, a standard substance mother solution, an internal standard solution, a diluent, an extracting solution, a quality control substance and a redissolving solvent; S2, detecting the dehydroepiandrosterone in the pretreated saliva by adopting the high performance liquid chromatography-tandem mass spectrometry technology; S3, separating the dehydroepiandrosterone from the interferent by using high performance liquid chromatography; S4, quantifying by using an isotope internal standard method; S5, taking the concentration ratio of the standard substance to the internal standard substance as an X axis and the peak area ratio of the standard substance to the internal standard substance as a Y axis; and S6, establishing a standard curve, and calculating the content of the dehydroepiandrosterone in the sample to be detected. The method is high in sensitivity and good in specificity, saliva sample treatment is simple, methodology verification meets requirements, and it is indicated that the method is reliable and capable of being applied to quantitative detection of dehydroepiandrosterone in human saliva.

Description

technical field [0001] The invention relates to the technical field of detection, in particular to a method for detecting the content of DHEA in human body fluid. Background technique [0002] Dehydroepiandrosterone (DHEA) is a steroid hormone secreted by the adrenal cortex. It is a precursor for the synthesis of androgens and estrogens, and has a wide range of physiological and pathological effects. DHEA is secreted into the blood, and most of it is metabolized to form bound DHEA. These bound DHEA have weak physiological activity, while the remaining DHEA is called free DHEA, which mainly exerts physiological activity. Free DHEA in blood diffuses passively into saliva through salivary gland epithelial cells, so the content of DHEA in saliva is considered to be used to evaluate the level of free DHEA in humans. [0003] Traditional methods for detecting DHEA mainly include enzyme-linked immunoassay and chemiluminescence immunoassay, etc. These methods are prone to interfere...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/72G01N30/04G01N30/06G01N30/86G01N30/88G01N30/34
CPCG01N30/02G01N30/04G01N30/06G01N30/34G01N30/72G01N30/8634G01N30/88G01N2030/045G01N2030/062G01N2030/067G01N2030/8813G01N2030/884
Inventor 蔡俊吴建军
Owner 艾可泰科(浙江)控股有限公司
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