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Preparation method of sika deer skin collagen and collagen peptide

A technology of buckskin collagen and sika deer, applied in peptide preparation methods, chemical instruments and methods, animal/human proteins, etc., can solve the problems of cumbersome extraction process, chemical pollution, long extraction cycle, etc., and simplify the process flow , high stability and high extraction rate

Inactive Publication Date: 2021-07-02
LIAONING UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Collagen extracted from deerskin is less affected by antibiotics than ordinary livestock and poultry, and is a relatively high-end product. Sika deer has a long history in Northeast China and has become a pillar industry in the rural economy of Northeast China. However, the research and extraction of collagen from deerskin still remains in its infancy
[0003] Collagen extraction methods mainly include acid method, alkali method, neutral salt method and enzymatic extraction. The purity of collagen extracted by acid extraction method is low, the extraction process is cumbersome, and the extraction cycle is long. The extraction rate of alkali extraction method is not high and easy to extract. cause chemical pollution

Method used

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  • Preparation method of sika deer skin collagen and collagen peptide
  • Preparation method of sika deer skin collagen and collagen peptide
  • Preparation method of sika deer skin collagen and collagen peptide

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Experimental program
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Embodiment 1

[0041] Embodiment 1 A kind of preparation method of sika deer skin collagen

[0042] (1) preparation method comprises the steps:

[0043] 1) Deerskin pretreatment: mechanically dehair the sika deerskin, then use 5% sodium carbonate aqueous solution to remove fat for 24 hours, and use 5% sodium chloride to remove impurities for 24 hours.

[0044]2) Acid extraction: add a small amount of glacial acetic acid with a concentration of 0.5mol / L to the pretreated sika deer deer skin, after homogenizing with a homogenizer, according to the ratio of material to liquid, sika deer deer skin: glacial acetic acid = 1g: 20mL Ratio, add glacial acetic acid with a concentration of 0.5mol / L again, and extract at 4°C for 8h.

[0045] 3) Ultrasonic-assisted enzyme extraction: After adjusting the pH of the extract obtained in step 2) to 2.5, add pepsin at 4% by weight, firstly, under ultrasonic power 200W, ultrasonic temperature 37°C, ultrasonic treatment for 40min, and then under stirring Enzym...

Embodiment 2

[0079] Embodiment 2 A kind of preparation method of sika deer skin collagen

[0080] (1) including the following steps:

[0081] 1) Deerskin pretreatment: mechanically dehair the sika deerskin, then use 5% sodium carbonate aqueous solution to remove fat for 24 hours, and use 5% sodium chloride to remove impurities for 24 hours.

[0082] 2) Acid extraction: add a small amount of glacial acetic acid with a concentration of 0.5mol / L to the pretreated sika deer deer skin, after homogenizing with a homogenizer, according to the ratio of material to liquid, sika deer deer skin: glacial acetic acid = 1g: 20mL Ratio, add glacial acetic acid with a concentration of 0.5mol / L again, and extract at 4°C for 8h.

[0083] 3) Ultrasonic-assisted enzyme extraction: After adjusting the pH of the extract obtained in step 2) to 2.5, add pepsin at 4% by weight, firstly, under ultrasonic power 200W, ultrasonic temperature 37°C, ultrasonic treatment for 40min, and then under stirring Enzymolysis w...

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Abstract

The invention relates to a preparation method of sika deer skin collagen and collagen peptide. According to the method, the extraction rate is mainly taken as an index, the optimal extraction method is selected through different extraction methods to extract the collagen, namely, the collagen in the sika deer skin is coarsely extracted by adopting an ultrasonic-assisted enzyme extraction mode, so that the collagen with relatively high extraction rate is obtained, and the collagen peptide is prepared from the extracted collagen by selecting different enzymolysis. In the aspect of physicochemical properties, the collagen peptide prepared from the compound protease and the papain has better physicochemical properties, and in the aspect of functional properties, the collagen peptide prepared from the papain shows better oxidation resistance.

Description

technical field [0001] The invention relates to the technical field of collagen food, in particular to a preparation process of sika deer skin collagen and collagen peptide. Background technique [0002] Collagen, referred to as collagen, is a helical fibrous protein formed by three peptide chains. The molecule contains a large amount of glycine (about 25% to 30%), proline (about 12%) and hydroxyproline (about 10%) , exists in all multicellular animals in the form of biopolymers, is an important component of connective tissue, and has biological activity. Collagen is usually extracted from the skin and bone of slaughtered animals containing connective tissue, such as cattle and pigs, donkey hide and bovine Achilles tendon, and fish skin and fish bones in marine organisms. However, the thermal stability of collagen extracted from fish skin is low. Collagen extracted from deerskin is less affected by antibiotics than ordinary livestock and poultry, and is a relatively high-e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C12P21/06C07K1/14C07K1/30C07K1/34
CPCC07K14/78C12P21/06
Inventor 胡风庆窦丹彤
Owner LIAONING UNIVERSITY
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