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Preparation method and application of avocado oil rich in furan lipid unsaponifiable substances

A technology of saponifiable matter and avocado oil, which is applied in the field of preparation of avocado oil rich in furan lipid unsaponifiable matter, can solve the problems of no utilization, economic and environmental burden, waste of avocado resources, etc., and achieve the goal of reducing connective tissue damage effect

Active Publication Date: 2021-06-29
大连奥川生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Avocado oil has high nutritional value, but the distillate in the refining process of avocado oil is often disposed of as waste and not used rationally. Disposal creates an economic and environmental burden

Method used

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  • Preparation method and application of avocado oil rich in furan lipid unsaponifiable substances
  • Preparation method and application of avocado oil rich in furan lipid unsaponifiable substances

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] A preparation method of unsaponifiable matter, comprising the following steps:

[0060] (1) Preparation of avocado oil: Take the avocado, peel and remove the core of the avocado, beat the pulp, adjust the pH value to 3.5, adjust the temperature to 45°C, add 1.2% xylanase, and enzymatically hydrolyze for 40 minutes; then adjust the pH value to 7.4, add alkaline protease (the mass ratio of xylanase and alkaline protease is 3:1), after enzymolysis 50min, according to the avocado pulp mass, add the mixture of 1.0% phospholipase and ethanol (phospholipid The mass ratio of enzyme and ethanol is 1:4) to carry out demulsification, add in water bath, centrifuge and separate layers, and extract the upper layer oil; make avocado oil; wherein, the enzyme activity of the xylanase is 10000U / g; The enzyme activity of the alkaline protease is 500000U / g;

[0061] (2) Distill avocado oil at 0.12 mbar and a temperature of 160°C, take the distillate, add acidic alumina, and carry out cycl...

Embodiment 2

[0066] A preparation method of unsaponifiable matter, comprising the following steps:

[0067] (1) Preparation of avocado oil: Take the avocado, peel and remove the core of the avocado, beat the pulp, adjust the pH value to 3, adjust the temperature to 40°C, add 0.5% xylanase, and enzymatically hydrolyze for 60 minutes; then adjust the pH value to 7, add alkaline protease (the mass ratio of xylanase and alkaline protease is 1:1), after enzymolysis 40min, according to the mass of avocado pulp, add the mixture of 0.5% phospholipase and ethanol (phospholipid The mass ratio of enzyme and ethanol is 1:5) to carry out demulsification, add in water bath, centrifuge and separate layers, and extract the upper layer oil; make avocado oil; wherein, the enzyme activity of the xylanase is 5000U / g; The enzyme activity of the alkaline protease is 600000U / g;

[0068] (2) Take avocado oil and distill it at 0.1mbar at a temperature of 180°C, take the distillate, add acidic alumina, and carry o...

Embodiment 3

[0073] A preparation method of unsaponifiable matter, comprising the following steps:

[0074] (1) Preparation of avocado oil: take the avocado, peel and remove the core of the avocado, beat the pulp, adjust the pH value to 4, adjust the temperature to 50°C, add 2% xylanase, and enzymatically hydrolyze for 30 minutes; then adjust the pH value to 8, add alkaline protease (the mass ratio of xylanase and alkaline protease is 5: 1), after enzymolysis 60min, according to the mass of avocado pulp, add the mixture of 1.5% phospholipase and ethanol (phospholipid The mass ratio of enzyme and ethanol is 1:3) to carry out demulsification, water bath is shaken and added, centrifuged and layered, and the upper layer oil is extracted; avocado oil is obtained; wherein, the enzyme activity of the xylanase is 40000U / g; The enzyme activity of the alkaline protease is 300000U / g;

[0075] (2) Distill the avocado oil at 0.15 mbar and a temperature of 150°C, take the distillate, add hydrochloric a...

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Abstract

The invention belongs to the technical field of active ingredient extraction, and particularly relates to a preparation method and application of avocado oil rich in furan lipid unsaponifiable substances. The method comprises the steps of distillation, cyclization, saponification, extraction, washing, concentration and the like, and unsaponifiable substances can be effectively extracted by the process. The process method is simple and suitable for industrial popularization and application. The prepared unsaponifiable substances can be used for preparing medicines for treating arthritis, rheumatoid and periodontitis, and has a better effect.

Description

technical field [0001] The invention belongs to the technical field of active ingredient extraction, and in particular relates to a preparation method and application of avocado oil rich in furan lipid unsaponifiable matter. Background technique [0002] Unsaponifiable matter refers to substances that are insoluble in water but soluble in ether when oil is saponified, which does not work with alkali. Unsaponifiables include sterols, high-molecular fatty alcohols, saturated or unsaturated hydrocarbons, waxes, pigments, and vitamins, among which the most important components are squalene, tocopherol, and sterols. Most vegetable oils contain about 1% unsaponifiable matter. [0003] The content of unsaponifiable matter in vegetable oil is one of the indicators to identify the quality of oil. When vegetable oil is mixed with mineral oil and paraffin, its unsaponifiable value will increase. Therefore, the determination of unsaponifiable matter in oil can identify the purity and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/04C11B3/16C11B3/12C11B3/10A61K36/54A61P19/02A61P29/00A61P1/02
CPCC11B1/025C11B3/16C11B3/12C11B3/10A61K36/54A61K36/48A61P19/02A61P29/00A61P1/02A61K2236/31A61K2236/19A61K2236/37A61K2300/00
Inventor 张晓瑞
Owner 大连奥川生物科技有限公司
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