Self-amplification precisely-controlled indirect competitive immunochromatography test strip and application thereof

Abstract

The invention relates to a 'self-amplification' and 'precise control' indirect competitive immunochromatography detection test strip which consists of two parts, namely test paper and a sample cup. The test paper comprises a supporting layer, an adsorption layer fixed on the supporting layer and a protective layer. The adsorption layer sequentially comprises a sample pad, a combination pad, a cellulose membrane layer and a water absorption material layer at a handle end from a test end. A detection line and a quality control line are sequentially arranged on the cellulose membrane layer. The conjugate pad is made of glass fiber cotton adsorbed with a nano material labeled antigen. The sample cup is a container for diluting a sample, and a target monoclonal antibody is pre-fixed in the sample cup. According to the test strip, the antibody is independently dried in the sample cup, so that folding and damage in the antibody labeling process are avoided, and the antibody dosage can be accurately controlled; and nano-particle labeled artificial antigens are used as a probe, and each antibody intercepted on a detection line can be accurately combined with two labeled antigens, so that signal self-amplification is realized, and the sensitivity of the test paper is improved.

Description

technical field [0001] The invention relates to an immunochromatographic detection method, in particular to an indirect competitive immunochromatographic detection test strip with "self-amplification" and "precise control" for hapten detection and its application. Background technique [0002] Immunochromatography is a rapid immunoassay technology developed in recent years. Its basic principle is the interaction between antibodies and antigens. It uses nitrocellulose membrane as a carrier to label antigens or antibodies as a tracer. A chromatographic detection technique. Compared with traditional immunoassay methods, immunochromatography has the advantages of strong specificity, accurate detection results, simple equipment operation, rapid measurement, low cost, no need for skilled technicians or expensive equipment, etc., fully meeting the requirements of instant detection . At present, immunochromatography technology has been widely used in medicine, animal husbandry, ag...

AI Technical Summary

Problems solved by technology

[0003] In the field of food safety, small molecule antigen immunochromatography technology based on the principle of competition is the most widely used. However, the existing detection mode often encounters immune reagents such as antigens and antibodies. ELISA detection methods can be successfully established, but immunochromatographic test strips cannot be successfully established; And in many applications the sensitivity of the test strip still needs to be improved

The main reasons are as follows: 1. The antibody folded during the labeling process, and an excessive amount of antibody was added to stabilize the labeling material; 2. The efficiency of the artificial antigen intercepting antibody was low, and an excessive amount of labeled antibody had to be added in order to improve color development. Improve color development; 3. There is no signal amplification when colloidal gold is used to label antibodies. Fluorescent materials such as quantum dots achieve signal amplification while requiring detection instruments to judge the results, which complicates detection.

The existing small molecule immunochromatography detection mode cannot solve this problem, so a simple and sensitive test strip detection mode is urgently needed to provide a better tool for the development of immunochromatography test strips

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