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Pure natural compound amino acid small molecule active peptide extracted from plants and extraction method, preparation and application thereof

A technology of small molecule active peptides and compound amino acids, which is applied in the preparation methods of peptides, preparations for skin care, applications, etc., to achieve the effects of large sample loading, safe use and improved yield

Inactive Publication Date: 2021-06-25
云南孤狼翼族生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Those skilled in the art are eager to obtain a safe, pure natural compound amino acid small molecule active peptide extracted from plants, but this technical problem has not been solved

Method used

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  • Pure natural compound amino acid small molecule active peptide extracted from plants and extraction method, preparation and application thereof
  • Pure natural compound amino acid small molecule active peptide extracted from plants and extraction method, preparation and application thereof
  • Pure natural compound amino acid small molecule active peptide extracted from plants and extraction method, preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] S001 Preparation of purified protein: Take 50kg of dried kudzu rice, crush it, add 100kg of purified water, pour it into an extraction tank, heat it to 75±2°C, keep it warm for 15min, add 250ml of concentrated hydrochloric acid, stir well, cool down to 10°C, release the extraction The solution was centrifuged at 3000r / min, the supernatant was discarded, and the precipitate was collected to obtain the precipitated protein.

[0042] S002 Drying of purified protein: Take the above-mentioned precipitated protein and spread it on a smooth and clean flat stainless steel container, flatten the surface so that the thickness is about 1 cm, place it in a blast drying oven, and heat and dry it in a sealed environment at 78±2°C for 16 Hours, pulverized to obtain dry powder kudzu rice crude protein.

[0043] S003 Acid hydrolysis: Get the above-mentioned dried Gexianmi crude protein and add 6mol sulfuric acid concentration 2000ml per 1kg of dry Gexianmi crude protein, 110±5°C, hydrol...

Embodiment 2

[0048] S001 Preparation of purified protein: Take 50kg of dried kudzu rice, crush it, add 200kg to purify it, pour it into an extraction tank, heat it to 80±2°C, keep it warm for 20min, add 300ml of concentrated hydrochloric acid, stir well, cool down to 20°C, and release the extract , centrifuge at 4000r / min, discard the supernatant, and collect the precipitate to obtain the precipitated protein.

[0049] S002 Drying of purified protein: Take the above-mentioned precipitated protein and spread it on a smooth and clean flat stainless steel container, flatten the surface so that the thickness is about 2 cm, place it in a blast drying oven, and heat and dry it in a sealed environment at 80±2°C for 20 Hours, pulverized to obtain dry powder kudzu rice crude protein.

[0050] S003 Acid hydrolysis: Get the above-mentioned dry kudzu rice crude protein and add 6mol sulfuric acid concentration 2500ml per 1kg of dry kudzu rice crude protein, 120±5°C, hydrolyze for 9h, dilute with 2 time...

Embodiment 3

[0055] S001 Preparation of purified protein: Take 50kg of dried kudzu rice, crush it, add 300kg to purify it, pour it into an extraction tank, heat it to 85±2°C, keep it warm for 30min, add 350ml of concentrated hydrochloric acid, stir well, cool down to 30°C, and release the extract , centrifuge at 5000r / min, discard the supernatant, and collect the precipitate to obtain the precipitated protein.

[0056] S002 Drying of purified protein: Take the above-mentioned precipitated protein and spread it on a smooth and clean flat stainless steel container, flatten the surface so that the thickness is about 3 cm, put it in a blast drying oven, and heat and dry it in a sealed environment at 88±2°C for 26 Hours, pulverized to obtain dry powder kudzu rice crude protein.

[0057] S003 acid hydrolysis: get the above-mentioned dry kudzu rice crude protein and add 6mol sulfuric acid concentration 3000ml per 1kg of dry kudzu rice crude protein, 125 ± 5 ℃, hydrolyze 10h, dilute with 2 times o...

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Abstract

The invention belongs to the technical field of biology, and particularly relates to a pure natural compound amino acid small molecule active peptide extracted from plants and an extraction method, a preparation and application thereof. The pure natural compound amino acid small molecule active peptide extracted from Nostoc Sphaeroides comprises 16 compound amino acids, which are aspartic acid, serine, glutamic acid, glycine, histidine, arginine, threonine, alanine, proline, valine, methionine, lysine, isoleucine, leucine, phenylalanine and tryptophan. The first purpose of the invention is to provide a pure natural compound amino acid small molecule active peptide extracted from Nostoc Sphaeroides. The second purpose is to provide a method for extracting the pure natural compound amino acid small molecule active peptide from plants; the third purpose is to provide a preparation of the pure natural compound amino acid small molecule active peptide; the fourth purpose is to provide application of the pure natural compound amino acid small molecule active peptide.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a pure natural compound amino acid small molecule active peptide extracted from plants and its extraction method, preparation and application. Background technique [0002] Amino acid (Amino acid) is the basic unit of protein, which endows protein with a specific molecular structure and makes its molecules biochemically active. Proteins are important active molecules in organisms, including enzymes and enzymes that catalyze metabolism. Amino acid is the most basic substance that constitutes biological proteins and is related to life activities. It is the basic unit of protein molecules in organisms and has a close relationship with biological life activities. It has special physiological functions in antibodies and is one of the indispensable nutrients in living organisms. [0003] Bioactive peptide is a general term for different peptides from dipeptide to complex line...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/12C07K1/14C07K1/18C07K1/34C07K1/36A61K8/64A61Q19/00A61Q19/10A23L33/18A23J1/00A23J3/20A23J3/32A23J3/34
CPCC12P21/06C07K1/34C07K1/12C07K1/18C07K1/14C07K1/36A61K8/645A61Q19/10A61Q19/00A23L33/18A23J1/008A23J3/20A23J3/32A23J3/347A61K2800/805A23V2002/00A23V2250/55A23V2200/30
Inventor 钏助胜王建梅姜波李村红王硕王进霞肖丹杨敏
Owner 云南孤狼翼族生物科技有限公司
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