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Bovine placenta cell exosome freeze-dried powder and preparation method thereof

A technology of bovine placenta and exosomes, which is applied in the field of skin care products, can solve the problems of unstable exosome activity, complex reagent components, cumbersome preparation, etc., and achieve good integrity and biological activity, good stability and biological active effect

Pending Publication Date: 2021-06-25
GUANGZHOU SALIAI BIOLOGICAL GENETIC ENG CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the current freeze-drying method designed for exosomes can preserve the activity of exosomes to a certain extent, the components of the reagents are complex, the preparation is cumbersome, and the activity of exosomes is not stable, which is not conducive to the quality of exosomes. control

Method used

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  • Bovine placenta cell exosome freeze-dried powder and preparation method thereof
  • Bovine placenta cell exosome freeze-dried powder and preparation method thereof
  • Bovine placenta cell exosome freeze-dried powder and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1 Preparation of bovine placental cell exosome extract

[0054] (1) Placenta pretreatment: Remove the amniotic membrane and umbilical cord from the fresh placenta, wash it repeatedly until the blood is removed, drain the water and use it directly or store it in a freezer at -80°C.

[0055] (2) Placental tissue homogenate: placental tissue that is fresh or thawed to room temperature is washed twice with PBS buffer, processed into small pieces and put into a tissue homogenizer for homogenization. After the homogenization, the homogenate should be uniform mushy.

[0056] (3) Tissue freezing and thawing and filtrate collection: put the homogenized pasty tissue into a -80°C refrigerator and freeze and thaw repeatedly 2-3 times.

[0057] (4) Ultrasonic treatment: After thawing at room temperature, ultrasonically treat the homogenate for 30 minutes, add PBS buffer solution and stir thoroughly, filter through a filter, and store in a -80°C refrigerator.

[0058] (5) F...

Embodiment 2

[0060] Embodiment 2 Preparation of bovine placental cell exosome freeze-dried powder

[0061] Obtain bovine placental cell exosome extract according to the method of Example 1, thaw at room temperature, and excipient (3.0% mannitol, 0.4% trehalose, 0.3% glycine, 2.0% glycerol, 0.02% Tween, 0.2% white albumen, the rest is water) mixed according to the volume ratio of 1:1 to obtain a mixed solution, which is lyophilized in a sterile environment using Tofflon's lyophilizer according to the procedures in Table 1 (including pre-freezing, refrigeration, Vacuumizing, one-time sublimation, analytical drying), the maximum temperature during the freeze-drying process should not exceed 40°C, and the freeze-dried powder of exosomes from bovine placenta cells (Exo-01) was obtained.

[0062] Preparation process reference figure 1 ,Specific steps are as follows:

[0063] (1) Filling: Pour the prepared bovine placental cell exosome mixture into the barrel of the filling machine. Before star...

Embodiment 3

[0071] Embodiment 3 Preparation of bovine placental cell exosome freeze-dried powder

[0072] Obtain bovine placental cell exosome extract according to the method of Example 1, thaw at room temperature, and excipient (2.0% mannitol, 1.0% trehalose, 1.0% glycine, 1.0% glycerol, 0.05% Tween, 0.5% beetroot albumen, and the rest is water) according to the volume ratio of 1:1 to obtain a uniform mixture, then use a freeze dryer in a sterile environment to freeze-dry according to the procedures in Table 1 to obtain bovine placental cell exosome freeze-dried powder ( Exo-02).

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Abstract

The invention relates to the technical field of skin care products, in particular to bovine placenta cell exosome freeze-dried powder and a preparation method thereof. An excipient composed of mannitol, trehalose, glycine, glycerin, Tween, albumin and water is combined with the preparation technology, and the prepared bovine placenta cell exosome freeze-dried powder has good stability and biological activity and can be stored for a long time at normal temperature. Experiments show that the bovine placenta cell exosome still keeps good integrity and biological activity after being preserved at the normal temperature of 25 DEG C for 6 months, and is suitable for long-term preservation, transportation and application.

Description

technical field [0001] The invention relates to the technical field of skin care products, in particular to a freeze-dried bovine placental cell exosome powder and a preparation method thereof. Background technique [0002] Animal placenta contains a variety of biologically active substances such as proteins, amino acids, immunomodulatory peptides, mineral elements, vitamins, and various functional factors. These active substances have the functions of regulating immunity, anti-stress, and anti-oxidation. They are also used For a longer time and scope, for example, animal placenta products such as cattle and sheep have been included in the "Catalogue of Cosmetics Used Raw Materials", including hydrolyzed placenta (bovine) extract (HYDROLYZED PLACENTAL PROTEIN), animal placental protein (PLACENTAL PROTEIN) and so on. [0003] The placenta is rich in various types of stem cells, and the exosomes secreted by it, a vesicle with a diameter of 30-150 nanometers, contain various ty...

Claims

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Application Information

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IPC IPC(8): A61K8/99A61K8/98A61K8/34A61K8/60A61K8/64A61K8/44A61K8/49A61K8/02A61Q19/08
CPCA61K8/99A61K8/982A61K8/345A61K8/60A61K8/64A61K8/44A61K8/4973A61K8/022A61Q19/08
Inventor 陈海佳姜交华李学家徐记旺庞可亮陈东煌
Owner GUANGZHOU SALIAI BIOLOGICAL GENETIC ENG CO LTD
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