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Cultivation method of anoectochilus formosanus

A cultivation method and technology of golden clematis, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of long breeding cycle and low reproductive rate of golden clematis

Inactive Publication Date: 2021-06-25
福建晨曦种业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Therefore, in view of the above content, the present invention provides a method for cultivating clematis, which solves the problems of low reproductive rate and long breeding cycle of clematis in the prior art, accelerates the growth and reproduction of clematis seedlings, and realizes the cultivation of clematis clematis. large-scale breeding

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  • Cultivation method of anoectochilus formosanus
  • Cultivation method of anoectochilus formosanus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A cultivation method of gold line lotus, including the following steps:

[0040] (1) Treatment of the implant

[0041]Choose a strong generic, root-based, patient-free, anti-pest, remove the root and leaves, cut 1-2 cm with the stem segment as an explactor, rinse 40 min, then put it in soap water, and use soft The brush is cleaned and is cleaned with sterile water. It is placed on the ultra-net workbench in the ultra-net workbench, and then soaked in a 75% ethanol solution, so soaking for 6 min with a 0.1% chloride solution, and finally rinsing the reserve multiple times with sterile water. use;

[0042] (2) Induction of Urban

[0043] The handling of the treated explants were seeded to high temperature and high pressure sterilized induced medium for culture, and induced medium in MS-based medium, 6 g / L karam, 28 g / L sucrose, 0.05 mg / l zt, 0.6 mg / lnaa, 2.0mg / l 6-ba, 20g / L corn powder, 2 g / L expanded vermiculite composition, pH value of 6.0-6.5;

[0044] The cu...

Embodiment 2

[0052] A cultivation method of gold line lotus, including the following steps:

[0053] (1) Treatment of the implant

[0054] Choose a strong root, the root of the roots, the acute golden paste, go to the root and leaves, cut 1-2 cm with the stem segment as an explant material, rinse 60 min with flow, then put it in soap water for 9 minutes, and use soft The brush is cleaned; after rinse with sterile water, it is placed on the ultra-net table, soaked in a 75% ethanol solution, soaked in a 0.1% chloride solution for 7 minutes, and finally rinsing the reserve multiple times in sterile water. use;

[0055] (2) Induction of Urban

[0056] The treated explants were seeded into high-temperature high pressure sterilized induced medium for culture, and induced medium in MS-based medium, 7 g / l karam, 30 g / L sucrose, 0.15mg / L ZT, 1.2 mg / l NaA, 1.2 mg / l 6-ba, 25 g / L corn powder, 3 g / L expanded vermiculite composition, pH is 6.0-6.5;

[0057] The culture conditions of step (2) a...

Embodiment 3

[0065] A cultivation method of gold line lotus, including the following steps:

[0066] (1) Treatment of the implant

[0067] Choose a strong generic, root-based, patients with disease, go to the root and leaves, cut 1-2 cm with the stem segment as an explactor, flush 80min with flow, then put it in soap water, and use soft The brush is cleaned; after flushing with a sterile water, it is placed on the ultra-net table, soaking 30s in a 75% ethanol solution, then soaking 8 min with a 0.1% chloride solution, and finally rinsing the reserve multiple times with sterile water. use;

[0068] (2) Induction of Urban

[0069] The treated explants were inoculated into high-temperature high pressure sterilization-induced medium for culture, and induced medium was based on MS, and 8 g / l karam, 32 g / L sucrose, 0.2 mg / L ZT, 0.9 mg / l NaA, 1.6 mg / l 6-ba, 30 g / L corn powder, 4g / L expanded vermiculite composition, pH is 6.0-6.5;

[0070] The culture conditions of step (2) are: the temp...

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Abstract

The invention relates to the technical field of cultivation of traditional Chinese medicinal materials, and provides a cultivation method of anoectochilus formosanus. The cultivation method of the anoectochilus formosanus comprises the following steps: (1) carrying out treatment of explants; (2) carrying out induction of adventitious buds, specifically, taking MS as a basic culture medium of an induction culture medium, and adding carrageenan, cane sugar, ZT, NAA, 6-BA, corn protein powder and expanded vermiculite; (3) carrying out seedling cultures, specifically, taking MS as a basic culture medium of a seedling culture medium, and adding carrageenan, cane sugar, ZT, NAA, 6-BA, expanded vermiculite, a seafood wastewater extract and cucumbers; (4) transplanting, specifically, transplanting the tissue culture seedlings to a dark, wet and cool evergreen broad-leaved forest, and planting the anoectochilus formosanus by simulating an original ecological environment. According to the cultivation method of the anoectochilus formosanus, the problems that the anoectochilus formosanus is low in reproduction rate and long in reproduction period in the prior art are solved, growth and reproduction of anoectochilus formosanus seedlings are accelerated, and large-scale planting and breeding of the anoectochilus formosanus are achieved.

Description

Technical field [0001] The present invention relates to the field of cultivation techniques of Chinese herbal medicines, and more particularly to a cultivation method of gold line lotus. Background technique [0002] The Golden Line Lanke opened the liplant medicinal plant, and the folk articles were "medicine king", "God medicine", "golden grass", etc., can treat multiple diseases such as acute and chronic hepatitis, diabetes, have a wide range of diseases Medicinal value and health effect, and also a plant that is very high ornamental. Modern studies have shown that the Golden Lotus contains polysaccharide, flavonoids, organic acids, steroid compounds, alkaloids, a variety of trace elements and other active ingredients, with nourishing yin and fire, refinicure, anti-inflammatory analgesic analgesic analgesic. my country's golden line is mainly distributed in Zhejiang, Jiangxi, Fujian, Taiwan, Hunan, Guangdong, Hainan, Guangxi, Sichuan, Yunnan, Tibet, southeast, including Fujian...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 杨静民吴辉
Owner 福建晨曦种业有限公司
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