Rapid detection method for harmful algal bloom species molecule based on high-throughput sequencing

Abstract

The invention discloses a rapid detection method for harmful algal bloom species molecules based on high-throughput sequencing. The rapid detection method specifically comprises the following steps: (101) a standard strain screening step, (102) a gene information acquisition step, (103) a quantitative standard establishment step, (104) a high-throughput detection step and (105) a determination step. The invention provides the rapid detection method for harmful algal bloom molecules based on high-throughput sequencing, the method is developed by combining the scientific research and practice requirements of the method, is cost-saving, and can monitor the harmful algal bloom more efficiently and precisely.

Description

Technical field [0001] The present invention relates to the field of algal species detection, and more particularly, it relates to a rapid detection method of hazardous algae-based molecules based on high throughput sequencing. Background technique [0002] Due to the acceleration of the industry and the global integration process, since the 1970s, environmental pollution and climate change, especially nutritious salts, contaminants emissions and ship pressure caused by the surge in marine ships in the world. Factors have led to an increase in the frequency of hazardous algae, and the development of high latitude and spread throughout the world. Harmful algae has a frequent, long-term, toxic and disasterization worldwide. my country's Maritime Middle East Sea Sea is also deeply affected by harmful algae, is a healthy residents in my country, and the aquatic economy is affected by the most affected area. East China Origin, Ocean Origin, Mikai Kaya, Short Kaitan, Tama Mountain, Mou...

AI Technical Summary

Problems solved by technology

However, because phytoplankton, especially dinoflagellates, have large differences in molecular genetic information between different organisms and even different strains, the rapid molecular detection technology based on target algae strains isolated from different regions, especially foreign sea areas, is very difficult. Difficult to be directly applied to the molecular rapid detection of harmful algal blooms in my country

[0003] The current morphological observation and identification method has many defects and challenges as a routine means of daily water environment monitoring: (1) The occurrence of harmful algal blooms is usually caused by the explosive proliferation of some marine microalgae. The cell concentration of algal blooms is low, and some routine microscopic examination methods are difficult for qualitative and quantitative detection; (2) The causative species of harmful algal blooms tend to be miniaturized, and it is extremely difficult to identify them by optical microscopy, and often only the genus can be identified. In order to further accurately identify the species, electron microscope observation is often required.

The preparation process of electron microscope samples is cumbersome, and the requirements for sample preparation technology are high, and the ideal cell morphology may not be observed in the prepared samples; (3) Some harmful algal blooms have no cell wall and shrink after fixation , the shape changes greatly, and some cells even rupture directly after adding the fixative, which seriously affects the observation of the species populations causing harmful algal blooms, and cannot accurately determine their abundance; (4) the cell morphology of the species causing harmful algal blooms It is variable, and its appearance and morphological characteristics may easily change with the living environment and growth stage, which increases the difficulty of identifying the provenance of harmful algal blooms in natural water bodies in different sea areas; (5) The identification of the causative species of harmful algal blooms is highly dependent on experience, It is difficult for beginners and ordinary people to quickly master the identification technology and carry out accurate sample identification, but it takes several months or even years of training and experience to train an experienced technician who can relatively accurately identify on-site samples; (6) Morphological identification method Time-consuming and labor-intensive, it is difficult to perform rapid and accurate quantitative analysis on a large number of samples

Therefore, the manpower and material resources required for the monitoring of harmful algal blooms by analyzing water samples through morphological microscopy are enormous.

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