Method for preparing five anthocyanin standard substances from blueberry anthocyanin extract

Abstract

The invention relates to a method for preparing five anthocyanin standard substances from a blueberry anthocyanin extract, which comprises the following steps: treating a blueberry anthocyanin solution to be prepared containing five anthocyanin components through an organic filter membrane, and then carrying out semi-preparative high performance liquid chromatography treatment to obtain the five anthocyanin standard substances. The five kinds of anthocyanins are delphinidin-3-O-glucoside, cyanidin-3-O-glucoside, petunidin-3-O-glucoside, peonidin-3-O-glucoside and malvidin 3-O-glucoside. According to the method, the semi-preparative high performance liquid chromatography treatment conditions are optimized, so that different anthocyanin substances can be collected at different retention times, the method for simultaneously preparing five main anthocyanin standard substances from the blueberry anthocyanin extract is realized, the purity is high, and the yield is high; and moreover, the operation is simple and convenient, and the large-scale preparation of the anthocyanin in blueberries and the demand of the market on the anthocyanin monomers can be met.

Description

technical field [0001] The invention belongs to the technical field of preparing standard anthocyanins, and in particular relates to a method for preparing five kinds of anthocyanin standard products from blueberry anthocyanin extracts. Background technique [0002] Anthocyanins or anthocyanins are water-soluble natural pigments, and the basic structure core is 2-phenylbenzopyran, that is, Flavylium. There are more than 500 kinds of anthocyanins known in nature, of which 6 are the most common, namely cyanidin or hibiscus anthocyanin (Cyanidin), delphinidin or delphinidin, delphinidin ( Delphinidin), Peonidin, Petunidin or Petunidin, Malvidin and Pelargonidin. Anthocyanins generally exist in the form of glycosides, and often form anthocyanins or anthocyanins (Anthocyanin) through glycosidic bonds with one or more glucose, rhamnose, galactose, arabinose, etc., in which anthocyanin-3-O- Glucoside is the main anthocyanin. [0003] Anthocyanins are a group of compounds that ca...

AI Technical Summary

Problems solved by technology

This patented technology has the following disadvantages: (1) the anthocyanin monomer obtained by chromatographic separation needs to be subjected to low-temperature crystallization and recrystallization to reach 95% purity; (2) it is used for the preparation of black currant anthocyanin monomer, There are fewer anthocyanin types prepared (4 types, 2 of which are common); (3) different plant anthocyanin compositions are not the same, black currant has fewer anthocyanin types, and blueberry anthocyanin types are more (at least 13 species), blueberry anthocyanins cannot be separated well under the conditions used to separate blackcurrant anthocyanins; (4) the extraction and separation process of this patented technology is complicated, and it has undergone homogenate crushing enzymatic hydrolysis, negative pressure enhanced extraction, and medium pressure flash flash Chromatographic separation, etc., the production cost is high, and general laboratories cannot do it

This technology has the following deficiencies: (1) the process of chromatographic separation technology anthocyanin monomer is complicated, and multiple column chromatography techniques have been adopted, such as macroporous resin chromatography, solid phase extraction, gel chromatography, semi-preparative high-performance liquid phase Chromatography, resulting in the loss of more than 50% total anthocyanins, the recovery rate is low, the anthocyanins prepared are less (2 kinds), the purity is not high (less than 97%), and the production cost is higher

(2) The separation effect of this chromatographic separation technology is poor, and the five common main anthocyanins cannot be separated well

Images