Method and device for separating lysozyme from egg white
A separation device and separation method technology, applied in the direction of sterilization methods, biochemical cleaning devices, enzymology/microbiology devices, etc., can solve the problems of low yield, difficult large-scale production, limited purity, etc., and achieve improved Yield and purity, reduced operating costs, and the effect of reducing adsorption pollution
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[0055] In this embodiment, fresh eggs are washed and shelled, egg whites and egg yolks are separated by an egg separator, and the egg whites are collected. Take 100 mL of fresh egg white, add 900 mL of phosphate buffer containing 0.9% NaCl to dilute the fresh egg white, adjust the pH to 8.0 with 1.0 M HCl and NaOH, and slowly magnetically stir for 30 min. Then the evenly stirred egg white-phosphate buffer solution was refrigerated and centrifuged at 4°C at a speed of 9000r / min for 15 minutes to remove macromolecular viscous proteins and other impurity precipitates to obtain the supernatant, namely the egg white dilution. The egg white dilution enters a lysozyme separation device in egg white that is inherently coupled by membrane filtration and electrodialysis to further separate and concentrate the lysozyme in it, and vacuum freeze-dry the obtained lysozyme concentrate to obtain a white The powder is the finished lysozyme. The obtained lysozyme was detected by sodium dodecyl...
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