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Method and device for separating lysozyme from egg white

A separation device and separation method technology, applied in the direction of sterilization methods, biochemical cleaning devices, enzymology/microbiology devices, etc., can solve the problems of low yield, difficult large-scale production, limited purity, etc., and achieve improved Yield and purity, reduced operating costs, and the effect of reducing adsorption pollution

Active Publication Date: 2021-06-11
NANKAI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although this method can improve the recovery rate of lysozyme, the temperature of homogenization affects the activity of lysozyme to a certain extent; in addition, the egg white dilution after homogenization treatment is directly subjected to ultrafiltration treatment, and the ovomucin in the egg white Other active proteins with large molecular weight, such as ovalbumin and ovotransferrin, are easily adsorbed on the surface of the ultrafiltration membrane under the pressure drive, causing membrane surface pollution and affecting the long-term operation stability of the process
[0006] At present, the methods for isolating lysozyme from egg white have the problems of low separation efficiency, low yield, limited purity, easy fouling of the membrane and difficulty in large-scale production Therefore, there is an urgent need to develop a new method for the separation of lysozyme from egg white that is simple, efficient, stable in operation, and easy for industrial scale-up.

Method used

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  • Method and device for separating lysozyme from egg white
  • Method and device for separating lysozyme from egg white
  • Method and device for separating lysozyme from egg white

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Embodiment

[0055] In this embodiment, fresh eggs are washed and shelled, egg whites and egg yolks are separated by an egg separator, and the egg whites are collected. Take 100 mL of fresh egg white, add 900 mL of phosphate buffer containing 0.9% NaCl to dilute the fresh egg white, adjust the pH to 8.0 with 1.0 M HCl and NaOH, and slowly magnetically stir for 30 min. Then the evenly stirred egg white-phosphate buffer solution was refrigerated and centrifuged at 4°C at a speed of 9000r / min for 15 minutes to remove macromolecular viscous proteins and other impurity precipitates to obtain the supernatant, namely the egg white dilution. The egg white dilution enters a lysozyme separation device in egg white that is inherently coupled by membrane filtration and electrodialysis to further separate and concentrate the lysozyme in it, and vacuum freeze-dry the obtained lysozyme concentrate to obtain a white The powder is the finished lysozyme. The obtained lysozyme was detected by sodium dodecyl...

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Abstract

The invention provides a method and a device for separating lysozyme from egg white. The method is characterized in that egg white diluent obtained through dilution, pH regulation, homogenization, centrifugal precipitation and the like is used as a raw material, and the separation device formed by coupling membrane filtration and electrodialysis is used for synchronously separating and concentrating the lysozyme in the egg white. The device can comprise a plurality of basic working units, and each basic working unit comprises a feeding chamber and a lysozyme recycling chamber, wherein the feeding chamber is formed by alternately arranging a feeding chamber partition plate, a porous filter membrane, a recycling chamber partition plate and a cation exchange membrane. According to the device formed by coupling membrane filtration and electrodialysis, the process flow of lysozyme separation and extraction is shortened, the consumption of chemical reagents by processes such as a direct crystallization method and a polyelectrolyte precipitation method is avoided, and the secondary pollution caused by the consumption of a large amount of salt solution and acid-base by an ion exchange method and a resin adsorption method is also avoided; the production cost is saved, the environment is protected, and the method is suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of protein separation and extraction, and in particular relates to a method and a device for separating lysozyme from egg white. Background technique [0002] Lysozyme is a hydrolytic enzyme that can act on the cell wall of microorganisms. It has antibacterial and bacteriolytic effects, and has no toxic side effects. It can be used as a food preservative as a substitute for antibiotics and is widely used in wine, beverages, meat products and even It can be used in baby food to inhibit fungal microorganisms; it can also be used in fields such as bioengineering, medical treatment, health care and livestock and poultry feed, and its market demand is increasing year by year. Lysozyme is widely present in body fluids such as microorganisms, plants, and mammals, such as plasma, intestinal tract, urine, and milk, as well as egg whites of birds and poultry. Among them, the content of lysozyme in egg white is the mo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/36C12M1/12C12M1/42C12M1/00
CPCC12N9/2462C12Y302/01017C12M21/14C12M33/14C12M47/14C12M23/12
Inventor 卢会霞张陇卜绍峰
Owner NANKAI UNIV
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