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Bifidobacterium bifidum for relieving constipation and fermented food and probiotic preparation prepared from bifidobacterium bifidum

A bifidobacterium bifidum and microbial preparation technology, applied in the field of microbiology, can solve some problems such as inconspicuous functions and single function of bifidobacteria, and achieve the effects of increasing the effect, reducing the time of first black stool, and increasing the abundance

Active Publication Date: 2021-06-11
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Research on bifidobacteria for relieving constipation will have a huge impact on food science, microbiology, and preventive medicine. Due to the single function of bifidobacteria that has the effect of relieving constipation in the prior art and some functions are relatively small Not outstanding, therefore, research on the effect of bifidobacteria on constipation relief is needed

Method used

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  • Bifidobacterium bifidum for relieving constipation and fermented food and probiotic preparation prepared from bifidobacterium bifidum
  • Bifidobacterium bifidum for relieving constipation and fermented food and probiotic preparation prepared from bifidobacterium bifidum
  • Bifidobacterium bifidum for relieving constipation and fermented food and probiotic preparation prepared from bifidobacterium bifidum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0103] Example 1: Getting Bifidobacterium CCFM1167 in Bifidobacterium Bifidum

[0104] Specific steps are as follows:

[0105] 1. Separation screening of Bifidobacterium strains:

[0106] (1) Collect a female feces using a disposable sterile toilet, in a liquid medium containing the MRS + mass (0.05% -0.1%) of the MRS + mass (0.05% -0.1%) of the MRS + mass (0.05% -0.1%), in anaerobic incubator (N2: CO2: H2 = 80: 10: 10) Medium rich in 12h;

[0107] (2) After gradient dilution of fecal samples, the fecal sample was coated with a sterile saline, and the MRS + mass percent of 50U / ml uterocin was applied (0.05% -0.1%) l- The solid plate of cysteine ​​hydrochloride, cultured 24-48 h;

[0108] (3) Select a single bacteria that meets the basic form of the bifurcus to conduct flat-parallelline purification, screening separation of bifidobacteria selected strains;

[0109] (4) Cultivate the above-mentioned single colonies into a liquid MRS + mass (0.05% -0.1%) Culture 24 h in Cysteine ​​...

Embodiment 2

[0128] Example 2: Bifidobacterium CCFM1167 induced constipation-related symptoms for livotylamine

[0129] Specific steps are as follows:

[0130] (1) Preparation of two bifidobacteria CCFM1167 bacteria suspension

[0131] After the two bifidobacterium CCFM1167 strains were taken out of the -80 ° C refrigerator, in the MRS solid medium, 37 ° C culture was cultured, and the single colony was colored in the MRS liquid medium, and the culture was cultured at 37 ° C for 24 hours. liquid;

[0132] The prepared seed fluid was inoculated in a new MRS liquid medium at a new MRS liquid medium at 2% (V / V), and in 37 ° C for 24 h, it was again cultured in the same manner, and the production of two bifidobacteria CCFM1167 fermentation was prepared. liquid;

[0133] The prepared bifidobacterium CCFM1167 fermentation solution was then centrifuged at 6000 R / min, 4 ° C, and then resuspended with a mass volume ratio of 10% by degreasing milk, and was used for animal experiments.

[0134] (2) 2...

Embodiment 3

[0145] Example 3: Bifidobacterium CCFM1167 reduces constipation in serum of constipation in the serum of constipation (SS)

[0146] Specific steps are as follows:

[0147] (1) C57BL / 6J mice group, modeling and treatment method as in Example 2.

[0148] (2) After dead mice at the 40th day, the collected mouse blood was allowed to stand 2 h, and the serum was obtained by centrifugation for 15 minutes, and the serum was obtained, and the lactorin (SS) detection kit was used, and the experiment was carried out in accordance with the instructions, by the standard curve. Calculate the concentration of SS in serum.

[0149] Studies have shown that growth inhibitors can inhibit gastrointestinal motility and the secretion of digestive tract hormones.

[0150] Experimental results figure 2 As shown, the model group (the content of the growth chalk SS is 6.410 ng / L) has an upward trend compared with the control group (P = 0.0330), and the two bifidobacterium CCFM1167 groups (the content ...

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Abstract

The invention discloses bifidobacterium bifidum for relieving constipation as well as a fermented food and a probiotic preparation prepared from the bifidobacterium bifidum, and belongs to the technical field of microorganisms. The Bifidobacterium bifidum CCFM1167 provided by the invention can be used for effectively relieving the first black stool time, the intestinal tract propulsion rate and the water content of stool of a mouse with constipation, and has the effect of obviously improving the apparent pathological indexes of constipation; in the detection of physicochemical indexes, the bifidobacterium bifidum can also influence the content of gastrointestinal active peptides, so that the intestinal peristalsis is influenced; the bifidobacterium bifidum also can effectively relieve pathological injury of colon tissues so as to effectively relieve constipation symptoms, and compared with bifidobacterium longum GDMCC NO: 60941, the bifidobacterium bifidum has more obvious influence on MTL content, further has more obvious effect on increasing the small intestine propulsion rate, and is more suitable for constipation patients with weak small intestine propulsion.

Description

Technical field [0001] The present invention relates to a mitigation of constipation bifidobacteria and a fermented food and probiotics thereof, belonging to a microbial species. Background technique [0002] According to incomplete statistics, the prevalence of constipation in the world is about 20.0%, and the constipation of my country's adults is 7.0% -20.3%. At the same time, the prevalence of the Asia-Pacific region is 8.75%, and the prevalence of Western countries is as high as 27%; children and adolescents are 0.7-29.6%, and the prevalence of the elderly is 15-50%. It can be seen that constipation is more than just a disease with high prevalence, but also a wide range of water. [0003] With the changes in diet and changes in life, the number of constipation patients has more and more, although constipation will not directly threaten the safety of people, but the harm caused by this disease is not ignored. Specific manifestations: constipation can increase the original dig...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23C9/123A23L11/65A23L11/50A23L19/00A23L17/60A23L33/135A61K35/745A61P1/10C12R1/01
CPCC12N1/20A23C9/1234A23C11/106A23L19/00A23L17/60A23L17/65A23L33/135A61K35/745A61P1/10A23V2002/00A23V2400/517A23V2200/3204
Inventor 王刚王琳琳柴茂赵建新陈卫张灏
Owner JIANGNAN UNIV
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