Construction method and application of chronic heat stress bacterial infection animal model

An animal model and bacterial infection technology, which can be used in pharmaceutical formulations, compound screening/testing, preparations for in vivo experiments, etc., can solve the problems of weakened intestinal function and unclear changes in immunity, and shorten the time of heat stress , good simulation effect and prolonged heat stress days

Inactive Publication Date: 2021-06-11
FOSHAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

At present, there are many reports on the impact of heat stress on the immunity of broiler chickens, and mainly focus on the impact on the weight of immune organs spleen and bursa of Fabricius and the number of immune cells. There are also many reports on the changes of intestinal inflammation in broiler chickens infected with Salmonella , but the weakened intestinal function and immune changes are not clear

Method used

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  • Construction method and application of chronic heat stress bacterial infection animal model
  • Construction method and application of chronic heat stress bacterial infection animal model
  • Construction method and application of chronic heat stress bacterial infection animal model

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Experimental program
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Embodiment 1

[0052] Broilers were divided into 4 groups, namely control group (control), heat stress group (HS), Salmonella group (SE) and model group (HS+SE). Place broilers in a temperature-controlled (25°C) room on a 12-hour light-dark cycle (lights on from 7:00 to 19:00). Chickens had ad libitum access to a standard experimental diet and drinking water.

[0053] Then the heat stress group (HS) and the modeling group (HS+SE) were subjected to chronic heat stress modeling, and the chickens were put into a large constant temperature incubator for heat stress experiments. Shock for 8 hours, continuous heat stress for 14 days. Then the model group (HS+SE) was given 1mL Salmonella (1×10 9 cfu / mL).

[0054] The control group (control) and the Salmonella group (SE) were fed at 25°C, and 1 mL of Salmonella (1×10 9 cfu / mL), Salmonella group (SE) also administered 1mL Salmonella (1×10 9 cfu / mL).

[0055] On day 4 after infection with Salmonella, all animals were sacrificed and ileal tissues...

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Abstract

The invention discloses a construction method and application of a chronic heat stress bacterial infection animal model. The construction method comprises the following steps that 1) chronic heat stress is induced; and (2) bacteria are infected, wherein the bacteria are salmonella; and an animal is a chicken. According to the construction method of the animal model, model construction is carried out through two-factor processing, so that the simulation effect is better, and production requirements can be better met; the heat stress time is shortened every day, the number of days of heat stress is prolonged, and the influence of heat stress on the immune function of the broiler chicken and the influence of non-transition heat stress on the whole physiological state of a body are fully exerted; and the chicken is subjected to heat stress and then infected with the salmonella after heat stress, so that the body immune change after heat stress and the influence of the heat stress on salmonella infection can be better reflected.

Description

technical field [0001] The invention relates to a method for constructing an animal model of chronic heat stress bacterial infection and its application. Background technique [0002] Global warming has a greater impact on the entire livestock industry. As global temperatures increase, the negative impact of heat stress on livestock health and performance has become one of the major impact factors in the poultry farming industry. The suitable ambient temperature range for most poultry is 18-20°C. Heat stress outside this range will reduce their feed intake and increase their drinking water, resulting in reduced weight gain, low reproductive efficiency, low milk production, and susceptibility to disease [1] . The U.S. poultry industry reportedly loses an average of $128 million annually due to heat stress [2] . [0003] According to the intensity and duration of stress, it can be divided into acute heat stress and chronic heat stress. Long; mild heat stress usually cause...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02A61K49/00
CPCA01K67/02A61K49/0008
Inventor 唐陆平李伟豪刘逸雷何永明
Owner FOSHAN UNIVERSITY
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