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3D printing collagen biological sponge scaffold and preparation method thereof

A technology of collagen and original protein, which is applied in the direction of non-active components of polymer compounds, prostheses, additive processing, etc., can solve the problems of low viscosity of pure collagen, difficult to print and shape, etc., to facilitate cell growth and maintain survival rate , the effect of high cell viability

Pending Publication Date: 2021-06-08
NANJING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the viscosity of pure collagen is very low, making it difficult to print

Method used

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  • 3D printing collagen biological sponge scaffold and preparation method thereof
  • 3D printing collagen biological sponge scaffold and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Dissolve recombinant human collagen in 0.12M acetic acid aqueous solution to prepare 3% (wt%) recombinant human collagen solution; dissolve chitosan in 0.12M acetic acid aqueous solution to prepare 3% (wt%) shell Polysaccharide solution; in addition, proanthocyanidins were dissolved in phosphate buffer (pH=6) containing 10% (v / v) absolute ethanol to prepare a 0.3% (wt%) proanthocyanidin solution.

[0040] Under the condition of ice bath, the chitosan solution was dropped into the recombinant human collagen solution, and stirred during the period until the mass ratio of recombinant human collagen to chitosan was 1:1, and the total content was 1.5% (wt%) ), continue to stir for 20 minutes, vacuum degassing with a vacuum degassing machine, then inject the mixed solution into the printing bin, print and freeze forming layer by layer according to the CAD model at a temperature of -30 ° C, and then place it in a vacuum freeze dryer (Japan The FDU-1200 model of Tokyo Physical ...

Embodiment 2

[0042]Dissolve recombinant human collagen in 0.12M acetic acid aqueous solution to prepare 3% (wt%) recombinant human collagen solution; dissolve chitosan in 0.12M acetic acid aqueous solution to prepare 3% (wt%) shell Polysaccharide solution; in addition, proanthocyanidins were dissolved in phosphate buffer (pH=6) containing 10% (v / v) absolute ethanol to prepare a 0.3% (wt%) proanthocyanidin solution.

[0043] Under the condition of ice bath, the chitosan solution was dropped into the recombinant human collagen solution, and stirred during the period until the mass ratio of recombinant human collagen to chitosan was 4:1, and the total content was 1.5% (wt%) ), continue stirring for 20 minutes, vacuum degassing with a vacuum degassing machine, then inject the mixed solution into the printing bin, print and freeze forming layer by layer according to the CAD model at a temperature of -30 ° C, and then place it in a vacuum freeze dryer ( The FDU-1200 model of Tokyo Physical and C...

Embodiment 3

[0045] Dissolve recombinant human collagen in 0.12M acetic acid aqueous solution to prepare 3% (wt%) recombinant human collagen solution; dissolve chitosan in 0.12M acetic acid aqueous solution to prepare 3% (wt%) shell Glycan solution; in addition, proanthocyanidins were dissolved in phosphate buffer (pH6) containing 10% (v / v) absolute ethanol to prepare a 0.3% (wt%) proanthocyanidin solution.

[0046] Under the condition of ice bath, the chitosan solution was dropped into the recombinant human collagen solution, and stirred during the period, until the mass ratio of recombinant human collagen and chitosan was 1:4, and the total content was 1.5% (wt%) ), continue stirring for 20 minutes, vacuum degassing with a vacuum degassing machine, then inject the mixed solution into the printing bin, print and freeze forming layer by layer according to the CAD model at a temperature of -30 ° C, and then place it in a vacuum freeze dryer ( The FDU-1200 model of Tokyo Physical and Chemica...

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Abstract

The invention discloses a 3D printing collagen biological sponge scaffold and a preparation method thereof. The method comprises the steps that firstly, an acid solution containing chitosan and an acid solution containing collagen are mixed to be uniform to prepare 3D printing bio-ink, then the bio-ink is added into a printing gun head, and printing forming is conducted in a low-temperature environment according to design parameters and a model; and freeze-drying treatment is conducted on the freeze-formed product, the freeze-dried product is soaked in a solution containing procyanidine for cross-linking immobilization, and finally freeze-drying is performed after washing to obtain the recombinant human collagen biological sponge scaffold. By means of a low-temperature deposition 3D printing technology, all-environment temperature control can be achieved, and the forming stability is improved. The biological sponge scaffold has proper pores, forming controllability, good physical and chemical properties and biocompatibility, does not contain other impurities, and is low in manufacturing cost.

Description

technical field [0001] The invention belongs to the technical field of biological materials, and relates to a 3D printed collagen biological sponge support and a preparation method thereof. Background technique [0002] Collagen is the most common protein in the extracellular matrix (ECM) of animals. It is a triple helical structure composed of three intertwined polypeptide chains, which helps the growth and proliferation of cells in the body. However, pure collagen has a very low viscosity and is difficult to print. Therefore, it is usually necessary to add other substances to collagen to improve its viscosity problem. Chitosan is a naturally derived polysaccharide with excellent biodegradability, antifungal and antibacterial activities, and is non-cytotoxic. In addition, chitosan also contains the GAG ​​structural unit of N-acetyl-D-glucosamine, which is a good 3D printing material. In natural ECM, collagen is combined with glycosaminoglycans to improve its structural p...

Claims

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Application Information

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IPC IPC(8): A61L27/24A61L27/20A61L27/56A61K47/42C08J9/28C08J9/40C08L5/08C08L89/00B33Y70/10
CPCA61L27/24A61L27/20A61L27/56A61K47/42C08J9/28C08J9/40B33Y70/10C08J2389/00C08J2305/08C08J2201/0484C08L89/00C08L5/08
Inventor 杨树林朱帅金明杰周潮辉王小琼王子勋
Owner NANJING UNIV OF SCI & TECH
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