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Skin bio-printing ink as well as preparation method and application thereof

A bioprinting and ink technology, applied in the fields of biochemical equipment and methods, microorganisms, epidermal cells/skin cells, etc., can solve the problems of difficult batch preparation, complexity, high cost, and achieve the advantages of growth and differentiation, high cell survival rate, and high cost. The effect of sticking

Pending Publication Date: 2022-05-27
HEFEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, at present, the construction of personalized full-thickness skin (including epidermis and dermis) models based on bioprinting technology is still in its infancy. The dense epidermis cannot be well used in subsequent research
The patent application with publication number CN109054503A discloses a bioprinting ink preparation method, printing method and application, but the bioprinting ink is not suitable for skin bioprinting

Method used

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  • Skin bio-printing ink as well as preparation method and application thereof
  • Skin bio-printing ink as well as preparation method and application thereof
  • Skin bio-printing ink as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] The preparation of bioprinting ink containing dermal cells includes the following steps:

[0048] (1) After placing sodium alginate powder and gelatin powder under ultraviolet light for 30 minutes, add DMEM complete medium, the final concentration of sodium alginate is 2% (wt%), and the final concentration of gelatin is 10% (wt%) ), and then put the suspension in a 60°C oven for 1 hour to fully dissolve the sodium alginate and gelatin. After the mixture of sodium alginate and gelatin was cooled to room temperature, pasteurize it in a 60°C oven for 1 hour to obtain sodium alginate and Gelatin mixture, placed in a cell incubator for later use;

[0049] (2) Cell culture: First, suck out the original medium in the petri dish with a pipette; then, wash with PBS three times to remove the residual medium, add 2 mL of trypsin (0.25% Trypsin-EDTA) and put it back into the culture Digestion in the incubator for about 3 min. When the cells become round and some cells float, add 2...

Embodiment 2

[0052] The difference between this example and Example 1 is that the dermal layer cells are replaced with epidermal cells.

Embodiment 3

[0060] The difference between this example and Example 1 is that the final concentrations of sodium alginate, gelatin, laminin and their cells in the ink are: 1% (w / v), 10% (w / v), 200 μg / ml and 5×10 6 / ml.

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Abstract

The invention discloses a skin bio-printing ink, and relates to the technical field of skin tissue engineering, the skin bio-printing ink comprises a 50-200 [mu] g / mL laminin solution, and the laminin solution contains gelatin with the mass concentration of 3-10% and sodium alginate with the mass concentration of 0.5-2%. The invention also provides a preparation method and application of the skin bio-printing ink. The laminin solution disclosed by the invention has the beneficial effects that the laminin solution contains gelatin with the mass concentration of 3-10% and sodium alginate with the mass concentration of 0.5-2% to form skin bio-printing ink, proliferation of corium layer cells is facilitated, the survival rate of the corium layer cells is very high, growth and differentiation of epidermal layer cells are facilitated when full-thickness skin is further formed in the later period, and the skin bio-printing ink has a good application prospect. And by arranging the corium layer printing model, the epidermal layer and the corium layer are connected in a staggered manner, so that the formation of a wavy shape at the joint of the epidermal layer and the corium layer is facilitated, and the morphological structure of real skin is simulated.

Description

technical field [0001] The invention relates to the technical field of skin tissue engineering, in particular to a skin bioprinting ink and a preparation method and application thereof. Background technique [0002] The skin is the largest organ of the human body and plays a vital role in protecting the human body from the external environment. Since the skin is the body's first line of defense, it is vulnerable to different types of injuries (such as traumatic wounds, burns, diabetic foot, etc.). When the skin is severely damaged and cannot repair itself, the current main treatment method is autologous transplantation or tissue-engineered skin adjuvant therapy. Due to the limited autologous skin that can be transplanted, the development of tissue-engineered skin substitutes for the treatment of skin lesions is particularly important. In addition, European legislation bans the use of animals to test cosmetic ingredients and increases the number of industrial chemicals that...

Claims

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Application Information

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IPC IPC(8): C12N5/071
CPCC12N5/0698C12N5/0625C12N2533/52C12N2533/54C12N2533/74
Inventor 刘婧张伟张敏冯冲李谨
Owner HEFEI UNIV
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