Electroporation device and system

Abstract

The invention relates to an electroporation device. The electroporation device comprises an electrode holder, two electrodes and a gasket, wherein the two electrodes are oppositely mounted in the electrode holder; and the gasket is arranged between the two electrodes. The electroporation device further comprises a part / component capable of enabling an electric field generated by the electrodes to form non-uniform electric field intensity. According to the device disclosed by the invention, the distance between the electrodes can be set to be about 1 mm in the conventional order of magnitude with relatively low manufacturing and processing cost, the applied voltage required by an electroporation experiment is greatly reduced, the use safety is ensured, and the cell survival rate is higher than that of a traditional electroporation technology.

Description

technical field [0001] The invention relates to the technical field of cell electroporation, in particular to an electroporation device and system. Background technique [0002] Cell electroporation, also known as electrotransfection, is a commonly used method in cell transfection technology. Because the cell membrane is selectively permeable to external substances, the control of eukaryotic cell gene experiments requires the input of specific biological DNA and RNA fragments into eukaryotic cells. Applying a certain intensity of potential difference on both sides of the cell membrane and continuing for a period of time can generate micropores on the cell membrane and enhance the permeability of the cell membrane. When the cell membrane is electroporated, its permeability and membrane conductance will increase instantaneously, so that hydrophilic molecules, DNA, protein, virus particles, drug particles and other molecules that cannot pass through the cell membrane under nor...

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Problems solved by technology

[0003] The inventor found in the research that the existing electroporation devices can be divided into two types according to the electrode spacing: one is a standard electroporation cup with an electrode spacing of 1-4 mm, the electrode spacing is set on the millimeter scale, and the cell size is on the micron scale, so The applied voltage is large (generally from hundreds of volts to thousands of volts), and the electric field is not uniform. The electric field environment of different cells is not the same. The cells near the electrode are easy to die, and the cells in the weaker electric field cannot generate electricity Perforation; the other is an electroporation device with a micro-pitch of 1-100um. This device has a small electrode spacing, and the voltage to be applied is greatly reduced, which improves safety. However, due to the small electrode spacing of this device, the production cost It is relatively high, and generally requires micro-nano processing technology

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