Non-gynecological exfoliated cell preserving fluid, kit containing same and application

Abstract

The invention relates to the technical field of biomedicine, and particularly discloses a non-gynecological exfoliated cell preserving fluid, a kit containing the preserving fluid and application. The non-gynecological exfoliated cell preserving fluid is prepared from the following components in percentage by weight: 17-25% of a fixing agent, 2.5-3.5% of glycerol; 1.2%-1.5% of a hemolytic agent; 3.5%-4.5% of an anticoagulant; 0.2-0.4% of sodium chloride; and the balance of deionized water. The fixing agent comprises at least one of ethanol and methanol; the hemolytic agent is prepared from ammonium sulfate, trihydroxymethyl aminomethane and sodium salt; the preparation method comprises the following steps: uniformly mixing the deionized water, the anticoagulant, the sodium chloride and the glycerol, and then uniformly mixing the mixture with the fixing agent and the hemolytic agent to obtain the non-gynecological exfoliated cell preserving fluid. The kit containing the preserving fluid further comprises a diluent and an adhesive slide, the kit is applied to sputum cell series, needle suction and endoscope series, serous cavity effusion series and urine cell series. The preserving fluid has the capability of removing red blood cells.

Description

technical field [0001] The present application relates to the field of biomedical technology, in particular to a non-gynecological exfoliated cell preservation solution, a kit containing the preservation solution and applications. Background technique [0002] The role of exfoliated cell preservation solution is mainly to maintain and fix the original morphological structure of exfoliated cells; exfoliated cells refer to the mucosa on the inner surface of natural lumen organs. Epithelial cells are naturally exfoliated cells; non-gynecological exfoliated cells are mainly exfoliated cells in the respiratory tract, digestive tract, and pleural and ascites. [0003] All over the world, the morphological characteristics of exfoliated cells indicate important clinical symptoms of various diseases in the human body. The morphological examination of exfoliated cells can reveal early signs of disease occurrence, which can provide very powerful diagnostic clues and basis for clinical ...

AI Technical Summary

Problems solved by technology

[0004] For the above-mentioned related technologies, most of the current non-gynecological exfoliated cell preservation solutions mainly play the role of maintaining and fixing the original morphological structure of the exfoliated cells, but cannot remove more red blood cells, so that more red blood cells have a negative effect on the exfoliated cells. Interfering with clinical diagnosis

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