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Integral solution technical scheme for precise prevention and control of infectious spleen and kidney necrosis virus disease of mandarin fish

A technology of spleen and kidney necrosis virus and technical solutions, which is applied in the field of overall solution technical solutions for precise prevention and control of mandarin fish infectious spleen and kidney necrosis virus disease, and can solve the problems of lack of prevention and control measures

Inactive Publication Date: 2021-06-04
广州力必拓生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, most of the disease prevention and control technologies for aquatic animals, including mandarin fish, are only satisfied with the detection and detection of related pathogens, and there is a serious lack of a series of comprehensive technologies for corresponding prevention and control measures. )” is an overall technical solution for precise prevention and control of diseases

Method used

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  • Integral solution technical scheme for precise prevention and control of infectious spleen and kidney necrosis virus disease of mandarin fish
  • Integral solution technical scheme for precise prevention and control of infectious spleen and kidney necrosis virus disease of mandarin fish

Examples

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Effect test

Embodiment 1

[0045] On September 13, 2020, a farmer in Wuxi, Jiangsu, died of mandarin fishes that had been cultured for about 70 days, and the number of deaths per day was about 300. Afterwards, it was detected by fluorescent quantitative PCR that the death was caused by infectious spleen and kidney necrosis virus. According to the following The treatment plan deals with:

[0046] First stop feeding the feed.

[0047] On the first and second days, use "Shanze No. 1 500mL / mu + Herbal Dew 1000mL / mu" for two consecutive days;

[0048] On the 3rd and 4th day, use "Shanduqing No. 2 40g / mu" for two consecutive days.

[0049]On the 5th and 6th days, use "povidone-iodine solution (aquaculture) 330mL / mu+herbal dew 1000mL / mu for two consecutive days.

[0050] Feedback from customers: The number of deaths within 6 days after taking the drug changed significantly: from the initial more than 300 deaths per day to a few deaths per day, the death rate finally dropped to zero.

Embodiment 2

[0052] On September 21, 2020, mandarin fishes raised by a farmer in Zhaoqing, Guangdong Province for about 2 months died, and the number of deaths per day was about 500. Afterwards, it was detected by fluorescent quantitative PCR that the death was caused by infectious spleen and kidney necrosis virus. According to the The following treatment options address:

[0053] First stop feeding the feed.

[0054] On the first and second days, use "Shanze No. 1 500mL / mu + Herbal Dew 1000mL / mu" for two consecutive days;

[0055] On the 3rd and 4th day, use "Shanduqing No. 2 40g / mu" for two consecutive days.

[0056] On the 5th and 6th days, use "povidone-iodine solution (aquaculture) 330mL / mu+herbal dew 1000mL / mu for two consecutive days.

[0057] Customer feedback: Changes in the number of deaths within 6 days after medication: 500 / 200 / 100 / dozens, and the death rate was finally reduced to less than a few.

Embodiment 3

[0059] On November 27, 2020, a farmer in Zhongshan, Guangdong, died of mandarin fish that had been breeding for more than 40 days, and the number of deaths was about 200 per day. Later, it was detected by fluorescent quantitative PCR that the death was caused by infectious spleen and kidney necrosis virus. The following treatment options address:

[0060] First stop feeding the feed.

[0061] On the first and second days, use "Shanze No. 1 500mL / mu + Herbal Dew 1000mL / mu" for two consecutive days;

[0062] On the 3rd and 4th day, use "Shanduqing No. 2 40g / mu" for two consecutive days.

[0063] On the 5th and 6th days, use "povidone-iodine solution (aquaculture) 330mL / mu+herbal dew 1000mL / mu for two consecutive days.

[0064] Feedback from customers: The number of deaths within 6 days after taking the drug has changed significantly: from the beginning of more than 200 deaths per day, the number of deaths has finally dropped to a few per day.

[0065] From the above case anal...

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Abstract

The invention discloses an integral solution technical scheme for precise prevention and control of infectious spleen and kidney necrosis virus disease of mandarin fish. The scheme is as follows: a magnetic bead method virus DNA / RNA nucleic acid extraction kit (LBT-VDEK) is used, a 16-well fluorescent quantitative PCR instrument (LBT-16QPCR) and support equipment thereof are utilized, a PCR-fluorescent probe technology is used, and specific DNA nucleic acid fragments of infectious spleen and kidney necrosis virus of mandarin fish are quantitatively detected. The schem is simple, convenient and rapid, and has good specificity and high sensitivity. A modern traditional Chinese medicine technology and a micro-ecological technology are used. The infectious spleen and kidney necrosis virus of the mandarin fish is prevented and treated. The scheme can effectively reduce mortality, achieves accurate prevention and control, comprehensively, systematically and integrally solves the infectious spleen and kidney necrosis virus disease of the mandarin fish, and has a very wide use prospect and a very high practical value.

Description

technical field [0001] The invention relates to the prevention and control technology of aquatic animal diseases, and is mainly an overall technical solution for the precise prevention and control of mandarin fish infectious spleen-kidney necrosis virus disease. Background technique [0002] Mandarin fish is a famous freshwater cultured species in my country. With the success of mandarin fish artificial breeding and nursery technology, mandarin fish aquaculture has developed rapidly, and at the same time, disease problems have become increasingly serious. The diseases of mandarin fish mainly include viral diseases, bacterial diseases and parasitic diseases. Among them, viral diseases are prevalent rapidly and have a high incidence rate, and there are no effective control measures at present, which seriously affects the development of mandarin fish aquaculture. In recent years, large-scale explosive epidemics have occurred in farmed mandarin fish, resulting in a large numbe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/686C12N15/10A61K31/79A61K45/06A61P31/12
CPCC12Q1/701C12Q1/686C12N15/1013A61K31/79A61K45/06A61P31/12C12Q2563/107C12Q2545/114
Inventor 袁永青黄文文黄志坚陈总会
Owner 广州力必拓生物科技有限公司
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