A Weissella strain that promotes the production of guaiacols by Zygomyces rouckeri
A technology of Zygosaccharomyces rutheli and Weisseria, which is applied in the field of bioengineering, can solve the problems of insufficient output to improve the taste of soy sauce, uncontrollable influence of safety soy sauce flavor, and the like
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0036] Example 1: Effect of co-cultivation on the growth of Weissella enteroides and Zygomyces ruckeri
[0037] Weissella enteroides LCW-28 was statically cultured in MRS at 37°C for 24 hours to obtain a pre-culture. The preculture was inoculated into 100 mL of fresh MRS medium at an inoculum size of 2% (v / v). After static incubation for 24 h, the cells were collected by centrifugation at 10,000 g for 5 min, and resuspended in 4 mL sterile water. Transfer 1 mL of the suspension to a 50 mL centrifuge tube filled with 30 mL of MRS medium and grow statically at 37 °C for 4 h.
[0038] Zygomyces rouckeri was grown statically at 30° C. for 24 h in YPD medium containing 6% (m / v) NaCl to obtain a pre-culture. The preculture was inoculated into 100 mL of fresh YPD broth containing 6% (m / v) NaCl at an inoculum size of 2% (v / v). After static incubation for 24 h, the cells were collected by centrifugation at 10,000 g for 5 min, and resuspended in 4 mL sterile water.
[0039] For pure...
Embodiment 2
[0044] Example 2: Effects of co-cultivation of different microorganisms on the yield of guaiacols
[0045] Zygomyces ruckeri ZQ-01, Candida orthopsilosis, or Torulopsis glabrata were mixed with Weissella enterica-like LCW-28, Staphylococcus pasteuri ), Tetragenococcus halophilus, Bacillus cereus or Pediococcus acidilactici were co-cultured.
[0046] In a 100mL culture bottle containing 30mL MRS medium, add the co-cultivated bacterial solution (3.4×10 7 CFU / mL), cultivated at 37°C for 60 hours, and sucked the fermentation broth every 10 hours. The amount of guaiacols in the fermentation supernatant was detected.
[0047] Centrifuge the fermentation broth at 10000r / min for 5min at 4°C, collect the supernatant, add 5 times the amount of dichloromethane and 83.36μg·L -1 2-octanol was used as an internal standard for ultrasonic extraction for 15 min, and the organic phase was obtained through a separatory funnel, and the extraction was repeated twice. Add 5 g of anhydrous Na2SO...
Embodiment 3
[0051] Embodiment 3: Co-cultivation influences the expression of important genes on the synthetic 4-EG pathway
[0052] Centrifuge the cultured Saccharomyces ruxii suspension at 12000r / min for 3min at 4°C, discard the supernatant, put it in a centrifuge tube (treated with DEPC water) and put it in liquid nitrogen, and pour it into the pre- Grinding in a cold mortar, the bacteria were ground into a white powder, and transferred to a 1.5mL Eppendorf tube. Nasia kit was used for RNA extraction, and the reverse transcribed cDNA was used for THUNDERBIRD qPCR Mix Kit Follow the instructions of the instrument for qRT-PCR reactions. The primers used are shown in (Table 2).
[0053] When co-cultured for 4 h, the relative expression of genes had the largest difference, and the relative expression of aroB and aroF increased the most, which increased by 4.5 times and 3.2 times compared with the control group ( image 3 ). However, pheA, the main gene for the synthesis of phenylalani...
PUM
Login to view more Abstract
Description
Claims
Application Information
Login to view more - R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap