Supercharge Your Innovation With Domain-Expert AI Agents!

Rassf4 as a target and application in the treatment of diabetes mellitus with non-alcoholic fatty liver disease and liver cancer

A fatty liver disease, non-alcoholic technology, applied in the field of bioengineering of drug preparation, can solve the problems of difficult treatment, unsatisfactory effect, targeted intervention, etc.

Active Publication Date: 2022-05-17
天津医科大学朱宪彝纪念医院
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing targeted drugs are mainly aimed at patients diagnosed with liver cancer, and targeted intervention cannot be performed at an earlier stage, so the treatment is more difficult and the effect is not satisfactory

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Rassf4 as a target and application in the treatment of diabetes mellitus with non-alcoholic fatty liver disease and liver cancer
  • Rassf4 as a target and application in the treatment of diabetes mellitus with non-alcoholic fatty liver disease and liver cancer
  • Rassf4 as a target and application in the treatment of diabetes mellitus with non-alcoholic fatty liver disease and liver cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] RASSF4 overexpression vector plasmid construction

[0065] 1.1 PCR amplification of RASSF4

[0066] (1) Prepare the following system in a PCR tube:

[0067]

[0068] (2) After the above system is configured, the centrifuge is centrifuged, and PCR equipment is used for amplification; the reaction conditions are as follows:

[0069] Pre-denaturation at 95°C for 5 minutes, denaturation at 95°C for 10 seconds, annealing at 62°C for 30 seconds, extension at 72°C for 2 minutes, 40 cycles;

[0070] (3) Add the sample to 6×DNA loading, and add it to the prepared 1% agarose gel, DNA electrophoresis instrument 150V, about 15 minutes, take the gel after electrophoresis to the dark room, and irradiate it under ultraviolet light Next, try to cut off the gel containing only the target band and put it into a clean 1.5ml EP tube.

[0071] 1.2 Cut rubber recycling

[0072] (1) Weigh the weight of the cut gel and add 300ul BM Buffer to 100mg gel;

[0073] (2) Heat the gel with Bu...

Embodiment 2

[0107] With RASSF4 as the active ingredient, adding pharmaceutically acceptable auxiliary materials, it can be prepared into liquid injections of various specifications according to conventional methods. There are various administration routes of RASSF4, such as injection administration, intracavitary administration and so on.

[0108] (1) Preparation of injections:

[0109] RASSF4 200 mg, mannitol 700 mg, PEG3000 10 mg, distilled water 100 ml, make the pH value 7.0-7.5, filter the filtrate to a concentration of 3 mg / ml, pack in 2 ml per ampoule, and freeze-dry to obtain the injection.

[0110] (2) Preparation of tablets:

[0111] RASSF4 10 mg, microcrystalline cellulose 35 mg, starch 45 mg, polyvinylpyrrolidone 4 mg, carboxymethyl starch sodium salt 4.5 mg, magnesium stearate 0.5 mg, talc 1 mg; RASSF4 active ingredient, starch and fiber sieve and mix thoroughly, mix the polyvinylpyrrolidone solution with the above powder, sieve to obtain wet granules and dry at 50°C, pre-si...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses the application of RASSF4 as a target drug for the treatment of diabetes complicated with non-alcoholic fatty liver disease to liver cancer in the treatment of liver inflammation, fibrosis and steatosis. Especially in the application of drugs that inhibit the proliferation, migration, invasion and tumorigenesis of liver cancer cells and promote apoptosis.

Description

technical field [0001] The invention belongs to the technical field of bioengineering for drug preparation, and relates to RASSF4 as a target for the treatment of diabetes combined with non-alcoholic fatty liver disease to liver cancer and its application. Background technique [0002] As a large country of liver cancer in my country, the existing treatment methods mainly include drug therapy, surgery, radiotherapy and chemotherapy; surgical treatment has a high recurrence rate, and radiotherapy and chemotherapy also have the disadvantage of relatively high toxicity; therefore, drug treatment is very important. Important, among which targeted drugs have always been the focus of research. The existing targeted drugs are mainly aimed at patients diagnosed with liver cancer, and it is impossible to carry out targeted intervention at an earlier stage, so the treatment is more difficult and the effect is not satisfactory. Therefore, it is necessary to find new drug targets for ta...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K45/00A61K48/00A61P3/10A61P1/16A61P35/00A61P35/04
CPCA61K45/00A61K48/005A61P3/10A61P1/16A61P35/00A61P35/04
Inventor 陈莉明孙蓓许朝飞
Owner 天津医科大学朱宪彝纪念医院
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com