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Forming mode of nano-silver coating on stem cell culture dish under assistance of magnetron sputtering and chemical gradient sedimentation

A technology of stem cell culture and magnetron sputtering, applied in the direction of sputtering plating, devices for coating liquid on the surface, ion implantation plating, etc., can solve the problem of not being able to effectively promote the repair and regeneration of damaged cells in wound healing Effectively activate and promote the growth of damaged tissue cells, and cannot effectively protect from being eroded by external microorganisms, etc., to achieve a strong antibacterial isolation effect, a strong antibacterial shielding effect, and the effect of promoting wound healing

Inactive Publication Date: 2021-05-28
西安美丽起点生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The stem cell culture dish in the prior art is an ordinary polymer material, which cannot effectively protect the stem cells from being eroded by external microorganisms, especially the erosive growth of bacteria during the process of culturing stem cells.
[0004] Moreover, ordinary polymer stem cell culture dishes cannot improve the microcirculation of the tissue around the wound, and at the same time cannot effectively promote wound healing and promote the repair and regeneration of damaged cells, and cannot eliminate the inflammatory response
Can not effectively activate and promote the growth of damaged tissue cells, reduce scar formation, and accelerate wound healing

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Stem cell culture dish made of polyethylene oxide PEO material, sterilized, cleaned by high-frequency oscillation, put the stem cell culture dish as the base material into the silver target, and wrap 860 turns of copper wire with a diameter of 0.1 cm on the outer target outside the vacuum chamber by magnetron sputtering. Clean the vacuum chamber, place the base material (stem cell culture dish) on the inner target, and close the vacuum chamber. Start to evacuate the vacuum chamber, the vacuum degree is 10 -6 , and input nitrogen filling into the vacuum chamber, control the voltage 220V, and the current value is 4A, and make the inner target form a silver nanofilm on the surface of the base material (stem cell culture dish) by magnetron sputtering, and use the silver target to bombard the base material stem cells The petri dish is bombarded for 20 minutes, the silver target is closed and cooled naturally for 20 minutes, and then the silver target is opened to bombard the...

Embodiment 2

[0058] Stem cell culture dish made of polyethylene oxide PEO material, sterilized, cleaned by high-frequency oscillation, put the stem cell culture dish as the base material into the silver target, and wrap 860 turns of copper wire with a diameter of 0.1 cm on the outer target outside the vacuum chamber by magnetron sputtering. Clean the vacuum chamber, place the base material (stem cell culture dish) on the inner target, and close the vacuum chamber. Start to evacuate the vacuum chamber, the vacuum degree is 10 -6 , and input nitrogen filling into the vacuum chamber, control the voltage 220V, and the current value is 4A, and make the inner target form a silver nanofilm on the surface of the base material (stem cell culture dish) by magnetron sputtering, and use the silver target to bombard the base material stem cells The petri dish was bombarded for 17 minutes, and the silver target was closed to cool naturally for 28 minutes. Then open the silver target and bombard the bas...

Embodiment 3

[0060] The PEO-like polymer stem cell culture dish is sterilized and cleaned by high-frequency oscillation, and the stem cell culture dish is placed into the silver target. Through electromagnetic sputtering, bombard the target silver target, wind 860 turns of copper wire with a diameter of 0.1 cm on the outer target outside the magnetron sputtering vacuum chamber, clean the vacuum chamber, and place the base material (stem cell culture dish) on the inner target, Close the vacuum chamber. Start to evacuate the vacuum chamber, the vacuum degree is 10 -6 , and fill the vacuum chamber with helium, control the voltage 220V, and the current value is 4A, and make the inner target form a silver nanofilm on the surface of the base material (stem cell culture dish) by magnetron sputtering, and use the silver target to bombard the base material 20min, turn off the silver target and let it cool naturally for 20min. Then open the silver target and bombard the base material (stem cell cu...

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Abstract

The invention discloses a forming mode of a nano-silver coating on a stem cell culture dish under assistance of magnetron sputtering and chemical gradient sedimentation. The forming mode comprises the following steps: a, disinfecting the stem cell culture dish, and carrying out high-frequency oscillation cleaning; b, placing the stem cell culture dish in a steady state, removing a three-dimensional maximum diameter under a horizontal condition, customizing a silver target, and placing the stem cell culture dish into the silver target; c, bombarding the target object silver target through electromagnetic sputtering, winding 860 copper wire turns with the diameter of 0.1 cm on an outer target on the outer side of a magnetron sputtering vacuum chamber, and cleaning the vacuum chamber; placing a substrate material (the stem cell culture dish) in an inner target, sealing the vacuum chamber, and starting to vacuumize the vacuum chamber with the vacuum degree of 10 <-6 >; and d, inputting inert gas into the vacuum chamber for filling. The stem cell culture dish formed by the mode has the effect of killing bacteria, fungi, mycoplasma, chlamydia, spirochetes and even trichomonad in clinical use, and does not generate drug resistance.

Description

technical field [0001] The invention relates to the technical fields of biomedicine, biomedicine, nanotechnology and chemical gradient sedimentation, in particular to a method for forming a nano-silver coating on a stem cell culture dish assisted by magnetron sputtering and chemical gradient sedimentation. Background technique [0002] Nanometer (nm) is the smallest unit of measurement after the micron in the physics world, with the development of nanotechnology. A large number of nanomaterials have been found to have antiphysical phenomena. As well as the quality and effect of special functions, nano-silver is also after the extensive development of metal nano-materials. an emerging field of research. Silver in the nanometer state has produced a qualitative leap in the bactericidal ability used in biomedicine. A very small amount of nano-silver can produce a powerful bactericidal effect that was unimaginable in the past. Nano-silver can kill more than 650 kinds of bacter...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C23C14/35B05D7/24C23C14/20
CPCB05D7/24C23C14/205C23C14/35
Inventor 刘哲
Owner 西安美丽起点生物科技有限公司
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