Sea cucumber saponin entrapped self-assembled nano system and preparation method and application thereof

A sea cucumber saponin and self-assembly technology, applied in the biological field, can solve the problems of reducing the hemolytic toxicity of sea cucumber saponins and the like

Active Publication Date: 2021-05-28
QINGDAO UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the use of 1,6-α-D-glucan to reduce the hemolytic toxicity of sea cucumber saponins

Method used

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  • Sea cucumber saponin entrapped self-assembled nano system and preparation method and application thereof
  • Sea cucumber saponin entrapped self-assembled nano system and preparation method and application thereof
  • Sea cucumber saponin entrapped self-assembled nano system and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038]1. Preparation of Palm Turluman Self-assembled nano-systems containing sea cucumber stocky

[0039](1) Preparation of palmitoylogloglucan: First, 1,6-α-D-glucan is dried, dried in the reaction bottle oven, DMF, pyridine, palmitoyl chloride chloride, to ensure reaction vessel , Samples, reagents are dry and water. Weigh 100 mg of anhydrous 1,6-α-D-glucan is dissolved in 8 ml of anhydrous N, N-dimethylformamide solvent, and heat it at 45 ° C to dissolve, add 1.0 ml of anhydrous pyridine and 30 mg. Palm chloride N, N-dimethylformamide solution 2 mL, magnetically stirred at 30 ° C for 80 h, and 1 ml of deionized water is added to terminate the reaction, and 3 times volume of anhydrous ethanol concentrate is added.

[0040](2) Isolation and purification of palmitoyloglucan: It is soluble in 1 ml of palm acylated dextran compound in 1 ml of deionized water, and purified by Sephadex G-25 gel penetration column chromatography, sulfate-phenol detection collection polysaccharide Component, el...

Embodiment 2

[0052]Inhibit the proliferation of tumor cells

[0053]1, material and method

[0054]1.1 experimental materials

[0055]Sample: The packaged sea cucumber saponin palmitoylated glucan self-assembly system is prepared in Example 1.

[0056]Neutrophic: 0.9% NaCl; positive plaque: Hydrochloride 5 μg / ml; White Reaction: Phosphate buffer solution.

[0057]Cell strain: liver cancer cells SMMC-7721, cervical cancer cells HELA, breast cancer cells MCF-7.

[0058]Reagents and instruments: trypsin, MTT, and enzyme label.

[0059]1.2 experimental method

[0060]1.2.1 Drug treatment dose and configuration method

[0061]The packaged sea cucumber pepperliminated dextran prepared in Example 1 was dispersed in a phosphate buffer solution, and the formulated concentration was 5 μg / ml, 10 μg / ml, 20 μg / ml, 40 μg / ml and 80 μg, respectively. / ml series of solutions.

[0062]1.2.2 Cell culture

[0063]Liver cancer cells SMMC-7721, cervical cancer cells HELA, breast cancer cells MCF-7 with DMEM culture containing 10% fetal bo...

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Abstract

The invention discloses a sea cucumber saponin entrapped self-assembled nano system and a preparation method and application thereof, and belongs to the technical field of biology. The preparation method comprises the following steps: firstly, reacting 1, 6-alpha-D-glucan with palmitoyl chloride to generate palmitoylated glucan, and separating and purifying to obtain a palmitoylated glucan compound; and dissolving the obtained palmitoylated glucan in ethanol to prepare a film, adding a phosphate buffer solution containing sea cucumber saponin, and stirring to form a palmitoylated glucan self-assembly system entrapped with sea cucumber saponin. The prepared palmitoylated glucan self-assembly system entrapped with the sea cucumber saponin has the entrapment rate of 69%, the hemolysis rate is less than 5% when the concentration of the sea cucumber saponin is 600mu g/mL, and the requirements of injection medicines are met. A cytotoxicity test proves that the palmitoylated glucan self-assembly system entrapped with the sea cucumber saponin has the effect of inhibiting tumor cells and can be used for preparing anti-tumor drugs.

Description

Technical field[0001]The present invention relates to the field of biotechnology, and in particular, to a self-assembled nano-based system containing sea cucumber stocky and its preparation methods and applications.Background technique[0002]Sea cucumber saponins is an important secondary metabolite product in the sea cell, with a variety of pharmacological activities such as anti-tumor and antifungal. In recent years, the good anti-tumor activity of sea cucumber is gradually attracted by everyone. However, enzymatic degradation of oral sea ginsenoside is susceptible to the digestive system affects its bioavailability. Intravenous injection of sea-ginsenoside, due to its combination with sterol molecules on the biofilm, causes hemolytic blood, and the above reasons limit the development and utilization of sea cucumber saponins as anti-tumor drugs. Therefore, hemolytic toxicity of the sea ginsenoside compound is of great significance for the development of its clinical anti-tumor drug...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K47/36A61K31/7048A61P35/00
CPCA61K9/5161A61K31/7048A61P35/00
Inventor 李红燕解万翠杨锡洪车红霞董秀芳宋琳
Owner QINGDAO UNIV OF SCI & TECH
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