Dry land soil denitrification culture device and application thereof

A cultivation device and a technology of denitrification, applied in the field of denitrification measurement, can solve problems such as restricting the research progress of dryland soil denitrification process, and achieve the effects of real-time high-resolution online measurement, ensuring airtightness, and improving accuracy

Pending Publication Date: 2021-05-25
INST OF SOIL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This greatly limits the research progress of dryland soil denitrification process, and it is urgent to develop new methods that can accurately quantify dryland soil denitrification process

Method used

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  • Dry land soil denitrification culture device and application thereof
  • Dry land soil denitrification culture device and application thereof
  • Dry land soil denitrification culture device and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] This embodiment will provide a denitrification cultivation device.

[0030] see Figure 1~3 , shows the dryland soil denitrification culture device of this embodiment, including a culture system, a gas system and an analysis and measurement system ( figure 1); in the present embodiment, the culture system is made up of 16 culture tanks with good airtightness; in the present embodiment, the diameter of the culture tank body is 16cm, and the height is 15cm; the bottom opening of the culture tank body is provided with a non-removable bottom The top opening is provided with a detachable top cover, and the bottom cover is provided with gas or substrate solution inlet and outlet pipelines, and the top cover is provided with an air inlet pipe and an exhaust pipe ( figure 2 ); the bottom of the tank is provided with a porous plate made of quartz stone and a filter membrane made of polyamide; a silicone rubber ring on the top of the tank is bonded to the top cover through high...

Embodiment 2

[0034] In order to make the purpose of the present invention, the technical bill and the advantages clearer, this embodiment will use the device of embodiment 1 to demonstrate the specific implementation effect of an automatic online measurement method for denitrification gas flux, as follows:

[0035] The 0-20cm topsoil under the long-term facility vegetable planting system near the Changshu Agricultural Ecological Experiment Station in the Taihu Lake Basin was collected. After the soil was collected, it was sealed with a sealing bag and sent to the laboratory as soon as possible. In the experiment, potassium nitrate solution and straw were added as nitrogen and carbon sources respectively, and three treatments were set up, respectively as follows: 1) blank control treatment (without adding any substrate), 2) straw treatment (adding 3g·kg -1 Straw dry soil calculation, 3) straw + potassium nitrate treatment (add 3g·kg -1 Straw dry soil, and add 20mM potassium nitrate, equival...

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Abstract

The invention discloses a dry land soil denitrification culture device and application thereof. The dry land soil denitrification culture device comprises a culture system, a gas path system and an analysis and determination system. The culture system consists of at least one culture tank body, a detachable top cover is arranged at the upper part of the tank body, an air inlet pipe and an exhaust pipe are arranged on the top cover, a fan is arranged in the tank body, a perforated plate and a polyamide filter membrane are arranged at the lower part in the tank body, and the polyamide filter membrane covers the perforated plate; a gas or substrate solution inlet and outlet pipeline penetrating through the bottom is arranged at the lower part of the tank body; the gas path system comprises a synthetic air source device and a gas mass flow controller, an exhaust port of the synthetic air source device is connected with a gas inlet pipeline of the gas mass flow controller, and an exhaust port of the gas mass flow controller is connected with a gas inlet pipe of the culture tank body through a first 16-position porous channel valve control system; the analysis and determination system comprises a gas chromatograph. The exhaust pipe of the culture tank body is respectively connected with the sample introduction end of the gas chromatograph through a second 16-position porous channel valve control system.

Description

technical field [0001] The invention belongs to the technical field of denitrification measurement, and in particular relates to an upland soil denitrification cultivation device and an automatic on-line determination method of denitrification gas flux. Background technique [0002] The denitrification process is the most important way for reactive nitrogen to leave soil, water and other endogenous cycles in the form of inert nitrogen and return to the atmosphere. On the one hand, the denitrification process converts reactive nitrogen into N 2 The form of denitrification returns to the atmosphere, which plays a role in closing the global nitrogen cycle and reducing the environmental effects of reactive nitrogen. On the other hand, the denitrification process will also cause the loss of nitrogen fertilizer in the farmland ecosystem and have an adverse impact on global climate change. [0003] In the previous studies on denitrification process, most of them only focused on th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N33/24
CPCG01N30/02G01N33/24G01N2030/025G01N33/245
Inventor 单军魏志军李承霖颜晓元
Owner INST OF SOIL SCI CHINESE ACAD OF SCI
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