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Construction method of photoelectrochemical aptamer sensor for sensitive detection of enrofloxacin

An aptamer sensor, photoelectrochemical technology, applied in the field of electrochemical detection

Active Publication Date: 2021-05-18
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Nitrobismuth oxybromide (N-Bi 4 o 5 Br 2 ) nanocomposites as photoelectric active materials to establish a photoelectrochemical sensing platform for the photoelectrochemical detection of enrofloxacin in lake water has not yet been reported

Method used

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  • Construction method of photoelectrochemical aptamer sensor for sensitive detection of enrofloxacin
  • Construction method of photoelectrochemical aptamer sensor for sensitive detection of enrofloxacin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Bismuth oxybromide (Bi 4 o 5 Br 2 )Nanosheets

[0032] First, 1.33g bismuth bromide (BiBr 3 ) was dissolved in 50mL of ethylene glycol, and 2mol / L NaOH12.6mL was added dropwise with stirring, then transferred to a polytetrafluoroethylene autoclave, and reacted at 140°C for 12h. After the reactor was cooled to room temperature, we washed the obtained samples three times with ethanol and deionized water, centrifuged them at 9000rmp for 8min in the laboratory, and finally dried them in an oven at 60°C for 12h. According to this method, Bi 4 o 5 Br 2 Nanosheets.

[0033] (2) Preparation of N-Bi 4 o 5 Br 2 nanocomposite

[0034] Weigh 0.2g Bi 4 o 5 Br 2 Mix with 0.02g urea, heat in a muffle furnace at 220°C for 3h, and wash the obtained compound with dimethyl sulfoxide and distilled water. Then dry at 60°C for 12h to obtain 10%N-Bi 4 o 5 Br 2 .

[0035] Different amounts of urea (0.04g, 0.06g, 0.08g and 0.1g) can be prepared according to the same steps...

Embodiment 2

[0042] (1) Bismuth oxybromide (Bi 4 o 5 Br 2 )Nanosheets

[0043] First, 2.66g bismuth bromide (BiBr 3 ) was dissolved in 50mL of ethylene glycol, and 2mol / L NaOH25.2mL was added dropwise with stirring, then transferred to a polytetrafluoroethylene autoclave, and reacted at 140°C for 12h. After the reactor was cooled to room temperature, we washed the obtained samples three times with ethanol and deionized water, centrifuged them at 9000rmp for 8min in the laboratory, and finally dried them in an oven at 60°C for 12h. According to this method, Bi 4 o 5 Br 2 Nanosheets.

[0044] (2) Preparation of N-Bi 4 o 5 Br 2 nanocomposite

[0045] Weigh 0.4g Bi 4 o 5 Br 2 Mix with 0.04g urea, heat in a muffle furnace at 220°C for 3h, and wash the obtained compound with dimethyl sulfoxide and distilled water. Then dry at 60°C for 12h to obtain 10%N-Bi 4 o 5 Br 2 .

[0046] Different amounts of urea (0.08g, 0.12g, 0.16g and 0.2g) can be prepared according to the same steps...

Embodiment 3

[0049] (1) Bismuth oxybromide (Bi 4 o 5 Br 2 )Nanosheets

[0050] First, 1.33g bismuth bromide (BiBr 3 ) was dissolved in 50mL of ethylene glycol, and 2mol / L NaOH12.6mL was added dropwise with stirring, then transferred to a polytetrafluoroethylene autoclave, and reacted at 160°C for 10h. After the reactor was cooled to room temperature, we washed the obtained samples three times with ethanol and deionized water, centrifuged them at 9000rmp for 8min in the laboratory, and finally dried them in an oven at 60°C for 12h. According to this method, Bi 4 o 5 Br 2 Nanosheets.

[0051] (2) Preparation of N-Bi 4 o 5 Br 2 nanocomposite

[0052] Weigh 0.2g Bi 4 o 5 Br 2 Mix it with 0.02g urea, heat it in a muffle furnace at 200°C for 5h, and wash the obtained compound with dimethyl sulfoxide and distilled water. Then dry at 60°C for 12h to obtain 10%N-Bi 4 o 5 Br 2. Different amounts of urea (0.04g, 0.06g, 0.08g and 0.1g) can be prepared according to the same steps to ...

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Abstract

The invention provides a construction method of a photoelectrochemical sensor for sensitive detection of enrofloxacin (ENR). The construction method comprises the following steps: step 1, preparing a bismuth oxybromide (Bi4O5Br2) nanosheet; step 2, preparing an N-doped bismuth oxide bromide (N-Bi4O5Br2) nano compound; and step 3, constructing the photoelectrochemical sensor for sensitively detecting enrofloxacin (ENR). Compared with a traditional detection method, the ENR photoelectrochemical detection method provided by the invention has the characteristics of simplicity, convenience and flexibility in operation, simplicity in instrument and equipment, wide detection range, low detection limit, low detection cost and the like.

Description

technical field [0001] The invention belongs to the field of electrochemical detection, and refers to a construction method and application of a photoelectrochemical aptamer sensor for detecting enrofloxacin in lake water. Background technique [0002] Enrofloxacin (ENR) is a fluoroquinolone antibiotic, which is a special quinolone antibacterial drug for livestock, poultry and aquatic products, and is widely used in human activities. However, increased ENR levels found in aquatic environments can have serious health consequences for humans and livestock. The European Commission and the Chinese Ministry of Agriculture stipulate that the maximum residue limit (MRLs) of ENR and its active metabolite ciprofloxacin in animal muscle tissue is 100 μg / kg, while the US Food and Drug Administration completely prohibits its use in poultry. Some antibiotic detection methods have been developed, including high performance liquid chromatography (HPLC), liquid chromatography-electrospray ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/15
CPCG01N33/15Y02A50/30
Inventor 王坤王媛郝楠戴震
Owner JIANGSU UNIV
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