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Application of metabolite ADMA in preparation of osteoarthritis diagnostic kit

A diagnostic kit and osteoarthritis technology, applied in the field of analytical chemistry and clinical medicine, can solve the problems of high cost, unfavorable early detection and intervention of osteoarthritis, and achieve high specificity, no radioactive hazard, and fast detection speed Effect

Active Publication Date: 2021-05-11
THE AFFILIATED SIR RUN RUN SHAW HOSPITAL OF SCHOOL OF MEDICINE ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, imaging tests are usually accompanied by a certain amount of radiation, or have a relatively high cost.
However, early osteoarthritis has no obvious clinical symptoms and imaging findings, which is not conducive to the early detection and intervention of osteoarthritis.

Method used

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  • Application of metabolite ADMA in preparation of osteoarthritis diagnostic kit
  • Application of metabolite ADMA in preparation of osteoarthritis diagnostic kit
  • Application of metabolite ADMA in preparation of osteoarthritis diagnostic kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A kit for arthritis, including kit including ADMA (asymmetric dimethylarginine) powder, ADMA-D7 (asymmetric dimethylarginine isotope-labeled internal standard), formic acid, acetonitrile and ultrapure Deionized water.

[0027] The arthritis kit can detect the content of the small molecule metabolite ADMA in the joint fluid, so as to assist in the diagnosis of early arthritis. The mass spectrometry targeted analysis ion information is shown in Table 1 below.

[0028] Table 1. ADMA Targeted Assay Information Sheet

[0029]

[0030] The specific use process of the arthritis kit is as follows:

[0031] Step 1. Joint fluid sample collection. The joint fluid in the joint to be tested by the subject is collected.

[0032] Step 2. Sample Analysis

[0033] 2-1. Sample preprocessing

[0034] Inject the joint fluid sample obtained in step 1 into a 1.5mL ep tube, and centrifuge at 5000g for 10 minutes at 4°C to remove a small amount of cells, cell debris and flocculent sedi...

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Abstract

The invention discloses an application of metabolite ADMA in preparation of an osteoarthritis diagnostic kit. Existing osteoarthritis diagnosis methods depend on imaging examination and clinical manifestation. X-rays show joint gap stenosis, articular surface blurring, subchondral hyperplasia, articular cavity osteophyte formation, joint fusion and dislocation and the like. The metabolite ADMA is used as a marker for osteoarthritis diagnosis and is used for preparing a kit for diagnosing arthritis, particularly early arthritis. The arthritis kit is prepared from ADMA powder, ADMA-D7, formic acid, acetonitrile and ultrapure deionized water. The invention provides a kit capable of detecting arthritis, particularly early arthritis, by utilizing the high relevance between the content of metabolite ADMA in synovial fluid and the arthritis disease condition and taking the metabolite ADMA as a marker. The arthritis kit provided by the invention has the characteristics of high detection speed, good repeatability, high sensitivity and high specificity, and has no radioactive hazard.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry and clinical medicine, and in particular relates to the application of metabolite ADMA in the preparation of an osteoarthritis kit and the quantitative analysis method of metabolite ADMA. Background technique [0002] Osteoarthritis (OA), also known as degenerative osteoarthritis, is a chronic degenerative disease characterized by articular cartilage degeneration, wear, synovial inflammation, and osteophyte hyperplasia. disease. It occurs frequently in middle-aged and elderly people, and its clinical manifestations mainly include joint pain, swelling and limited mobility. According to the latest Chinese epidemiological survey data, the elderly over 65 years old have a higher prevalence of osteoarthritis, among which the imaging prevalence rate of males is 27%, and the clinical prevalence rate is 7%. The sickness rate is as high as 46%, and the clinical prevalence rate reaches 15%. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/72
CPCG01N30/02G01N30/72
Inventor 吴奕征谢子昂秦安沈舒滢范顺武
Owner THE AFFILIATED SIR RUN RUN SHAW HOSPITAL OF SCHOOL OF MEDICINE ZHEJIANG UNIV
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