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Polydopamine-loaded hemoglobin micro-nano particle as well as preparation method and application thereof

A hemoglobin and polydopamine technology, applied in the field of hemoglobin oxygen carrier blood substitutes, can solve the problems of hemoglobin structure and function instability, hemoglobin autoxidation intensified, and oxygen-carrying capacity damage, etc., to provide anti-oxidative damage, The preparation conditions are simple and the effect of avoiding biological toxicity

Active Publication Date: 2021-05-11
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] It can be seen that the above insufficient stability is an important reason for limiting the further development of template self-assembled HBOCs
In addition, due to the lack of the reductase system contained in natural red blood cells, the autooxidation of hemoglobin intensifies under physiological conditions, the conformation changes, and the oxygen-carrying capacity is impaired, that is, the structure and function of hemoglobin are unstable.

Method used

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  • Polydopamine-loaded hemoglobin micro-nano particle as well as preparation method and application thereof
  • Polydopamine-loaded hemoglobin micro-nano particle as well as preparation method and application thereof
  • Polydopamine-loaded hemoglobin micro-nano particle as well as preparation method and application thereof

Examples

Experimental program
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Embodiment

[0045] Example: Preparation of polydopamine-loaded hemoglobin micro-nano particles

[0046] Include the following steps:

[0047] (1) Prepare 10ml containing 0.1-0.3M MnCl 2 and 2.4-12.0mg / ml hemoglobin, quickly add 0.1-0.3M Na at 4°C 2 CO 3 solution, stir until the reaction system is brick red, and there is no more precipitation, generally 5-10min; Mn 2+ Combined with hemoglobin and then with CO 3 2- MnCO 3 Precipitation, with Mn 2+ There is interaction of hemoglobin with Mn 2+ with CO 3 2- The combination is loaded into MnCO 3 , forming Hb-MnCO 3 MPs; centrifuge at 6000-10000g for 5-15min, discard the supernatant, the precipitate is Hb-MnCO 3 MPs (i.e. primary particles).

[0048] (2), add Tris-HCl buffer solution (pH=8.0-8.8) to the precipitation that step (1) obtains, make the pH=8.0-8.8 of reaction system; Then add 1-10mg / mL dopamine solution, dopamine and hemoglobin The addition ratio is 1: (1-5), continue to stir; based on the characteristic absorption o...

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Abstract

The invention discloses a polydopamine-loaded hemoglobin micro-nano particle and a preparation method and application thereof. The polydopamine-loaded hemoglobin micro-nano particle is obtained by wrapping hemoglobin with polydopamine, the micro-nano particle takes manganese carbonate as an adsorption template, hemoglobin is immobilized to form a primary particle, then a polydopamine adhesion layer is constructed on the surface of the primary particle, and finally, removing manganese carbonate to obtain the polydopamine-loaded hemoglobin micro-nano particle. The polydopamine-loaded hemoglobin micro-nano particle has a wide blood substitute application prospect due to a simple synthesis process, a complete chemical structure and good oxygen carrying capacity, blood compatibility, biological safety and oxidation resistance, and can play an important role in the field of medicines.

Description

technical field [0001] The invention relates to the technical field of blood substitutes of hemoglobin oxygen carriers, in particular to a polydopamine-loaded hemoglobin micro-nano particle and its preparation method and application. Background technique [0002] Timely and effective blood transfusion, as an important and common clinical medical treatment method, is widely used in surgical operations, medical anemia treatment and war trauma treatment. However, due to the increasingly serious transmission of blood-borne diseases, difficulties in storage, and cross-matching before treatment, it is difficult to meet the growing demand for blood transfusions, which has caused traditional blood transfusions to face major challenges. challenges, and at the same time provide an important opportunity for the research of blood substitutes. [0003] Blood substitutes can partially replace blood by simulating the structure and function of blood components. Because of its advantages of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K17/08C07K17/14
CPCC07K17/08C07K17/14
Inventor 赵莲王权周虹王瑛尤国兴胡吉林
Owner ACADEMY OF MILITARY MEDICAL SCI
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