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Hydrogel system used for capture and fixed-point releasing of circulating tumor cells

A tumor cell, hydrogel technology, applied in the field of biological hydrogel rice materials, can solve the problem of lack of reliable solutions

Active Publication Date: 2021-04-27
济南国科医工科技发展有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Photosensitive hydrogel systems have the potential to be applied to the capture and separation of circulating tumor cells, but there is a lack of reliable solutions

Method used

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  • Hydrogel system used for capture and fixed-point releasing of circulating tumor cells
  • Hydrogel system used for capture and fixed-point releasing of circulating tumor cells
  • Hydrogel system used for capture and fixed-point releasing of circulating tumor cells

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Experimental program
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Embodiment 1

[0037] This embodiment provides a hydrogel system for the capture and fixed-point release of circulating tumor cells. The hydrogel system is prepared by the following method:

[0038] 1) Preparation of Ti 3 C2 T x two-dimensional material

[0039] First prepare 6M hydrochloric acid (HCl) aqueous solution for later use, measure 10ml of HCl solution in a beaker, weigh 0.666g of lithium fluoride (LiF) and dissolve it in the HCl solution, place it on a stirring table and stir for 40 minutes; Remove it and pour it into a single-necked flask, place it in an oil bath at 35°C and preheat it with stirring, weigh 1g MAX-Ti 3 AlC 2 Dissolve in the flask solution, heat and stir for 24 hours to generate Ti 3 C 2 T x Material.

[0040] 2) Utilize Ti 3 C 2 T x Preparation of Ti from two-dimensional materials 3 C 2 T x monolayer nanosheet suspension

[0041] With the Ti obtained in step 1) 3 C 2 T x The two-dimensional material (black solution) was centrifuged three times in ...

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Abstract

The invention discloses a hydrogel system for capture and fixed-point release of circulating tumor cells. The hydrogel system is characterized by being prepared by the following method: 1) preparing a Ti3C2Tx two-dimensional material; (2) preparing Ti3C2Tx single-layer nanosheet suspension liquid by utilizing a Ti3C2Tx two-dimensional material; and 3) preparing a hydrogel film from the Ti3C2Tx single-layer nanosheet suspension and gelatin, performing surface carboxylation modification on the hydrogel film, and coupling an EpCAM antibody to obtain the hydrogel system. The hydrogel system provided by the invention has good photoresponse, thermal stability and cell adhesion, and specific capture of circulating tumor cells can be realized by modifying the surface of a hydrogel film with a coupling antibody; and the hydrogel system disclosed by the invention can be rapidly heated under the irradiation of laser with a specific wavelength, so that fixed-point release of circulating tumor cells can be realized.

Description

technical field [0001] The invention relates to the field of biological hydrogel rice materials, in particular to a hydrogel system used for the capture and fixed-point release of circulating tumor cells. Background technique [0002] Circulating rare cells has important implications for materials research and clinical applications. Among them, circulating tumor cells are the biomarkers of tumor "liquid biopsy"; non-invasive prenatal diagnosis can be realized by identifying and extracting circulating fetal nucleated red blood cells; others, such as circulating stem cells and endothelial cells, also have their own potential indicators in clinical diagnosis application. However, in the context of rich blood cells, it is challenging to characterize and isolate circulating rare cells with extremely low abundance. How to effectively capture and isolate circulating rare cells is a key research goal in the fields of biology and medicine. [0003] In recent years, due to its adjus...

Claims

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Application Information

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IPC IPC(8): C08J5/18C08J7/12C08L89/00C08K3/14C12N5/09
CPCC08J5/18C08J7/12C08J2389/00C08K3/14C08K2201/011C12N5/0693C12N2509/00
Inventor 董文飞尤倩楠梅茜葛明锋从瑛哥常智敏李力宁珊珊
Owner 济南国科医工科技发展有限公司
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