Method for screening hypha-state candida albicans virulence related genes

Abstract

The invention discloses a method for screening hypha-state candida albicans virulence related genes. The method comprises the following steps of: firstly, obtaining the candida albicans growing in a hypha phase by adopting a hypha induction experiment, then, analyzing the transcriptome difference of the candida before and after hypha induction by using a transcriptome sequencing method (RNA-seq), screening virulence related genes, and then, detecting and analyzing respective damage conditions of different host cells infected by the candida albicans by adopting a gram staining method and a cell proliferation rate; and finally, analyzing the difference of virulence gene expression after different types of host cells are infected by the candida by using a reverse transcription-fluorescent quantitative PCR method (qRT-PCR). According to the method, the associated relationship between hypha formation and some virulence factors of the candida can be defined; potential virulence related genes which are differentially activated when different cells are infected by the candida are screened out; and evidence is provided for illuminating different molecular mechanisms of candida infected hosts and screening high-specificity and high-reliability candida virulence genes for diagnosis.

Description

technical field [0001] The invention relates to the technical field of Candida albicans virulence-related genes, in particular to a method for screening mycelial Candida albicans virulence-related genes. Background technique [0002] Candida albicans ( Candida albicans ) are the most common human pathogens among fungi and are part of the normal flora of the skin, mucous membranes, and gastrointestinal tract of healthy hosts, however, they can be transformed into opportunistic Pathogenic killer, can cause superficial mucous membrane infection or systemic disease, and cause high mortality rate, in fact, the transmission of Candida is the leading cause of oropharyngeal disease in AIDS patients, vulvovaginal inflammation in women, as well as diabetic patients, premature infants and surgical patients. The main cause of invasive fungal disease in patients. This includes the direct contact between Candida albicans and epithelial cells to destroy epithelial cells; the implantation...

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Problems solved by technology

[0003] The purpose of the present invention is to provide a method for screening mycelial Candida albicans virulence-related genes, to solve the problem of Candida albicans respectively infecting human vaginal epithelial cells, human umbilical vein endothelial cells and human monokaryotic cells. Comparative analysis of Candida hyphae formation, cell adhesion, invasive enzyme expression and other virulence genes after virulent macrophages. This study has not been reported so far. In-depth study of the mechanism of action provides valuable basis and clues for the timely diagnosis of Candida virulence genes that change from a normal colonization state to a highly virulent infection state

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