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Preparation method of starch telluride-based bionic glutathione peroxidase

A technology of glutathione peroxide and starch, applied in the field of modified starch, can solve the problems of high cost and poor performance, and achieve the effects of stable structure and performance and excellent catalytic activity

Inactive Publication Date: 2021-04-20
BEIBU GULF UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The existing GPx simulant has high source of raw material and its high cost, which restricts its development to large-scale industrialization. Some raw materials have low cost and poor performance. In the prior art, there is no use of tellurized starch as a GPx simulant.

Method used

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  • Preparation method of starch telluride-based bionic glutathione peroxidase
  • Preparation method of starch telluride-based bionic glutathione peroxidase
  • Preparation method of starch telluride-based bionic glutathione peroxidase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The preparation method of telluride starch-based biomimetic glutathione peroxidase comprises the following steps:

[0033] 1) Weighing 30g of tapioca starch as a raw material, adding water to form a 30% starch emulsion, taking octenyl succinic anhydride with a dry weight of tapioca starch of 3.5%, and diluting it with 3 times the quality of absolute ethanol;

[0034] 2) Slowly drop octenyl succinic anhydride ethanol solution into the starch emulsion for reaction, use 3% NaOH solution to maintain the pH value of the reaction system to 8.5 during the reaction, and react at 40° C. for 4 hours;

[0035] 3) After the reaction, wash with deionized water and ethanol in sequence, filter, and dry to obtain starch octenyl succinate, that is, the OSA starch sample, and set aside;

[0036] 4) Take 0.1818g tellurium and 0.3636g potassium borohydride to react with 30mL water under nitrogen atmosphere to prepare sodium tellurium hydride solution for later use;

[0037] 5) Get 10g of ...

Embodiment 2

[0040] The preparation method of telluride starch-based biomimetic glutathione peroxidase comprises the following steps:

[0041] 1) Weigh 30g of tapioca starch as raw material, add water, configure 30% starch emulsion, take octenyl succinic anhydride with 3.5% dry weight of tapioca starch, and dilute with 4 times of absolute ethanol to obtain octenyl succinic anhydride Succinic anhydride ethanol solution for subsequent use;

[0042] 2) Slowly drop octenyl succinic anhydride ethanol solution into the starch emulsion for reaction, use 3% NaOH solution to maintain the pH value of the reaction system to 8.1 during the reaction, and react at 37° C. for 3 hours;

[0043] 3) After the reaction, wash with deionized water and ethanol in sequence, filter, and dry to obtain starch octenyl succinate, i.e. OSA starch sample, for subsequent use;

[0044] 4) Take 0.1818g tellurium and 0.7272g potassium borohydride and react with 30mL water under nitrogen atmosphere to prepare sodium tellur...

Embodiment 3

[0048] The preparation method of telluride starch-based biomimetic glutathione peroxidase comprises the following steps:

[0049] 1) Weigh 30g of tapioca starch as raw material, add water, configure 30% starch emulsion, take octenyl succinic anhydride with 3.5% dry weight of tapioca starch, and dilute it with 5 times of absolute ethanol to obtain octene Base succinic anhydride ethanol solution for subsequent use;

[0050] 2) Slowly drop octenyl succinic anhydride ethanol solution into the starch emulsion for reaction, use 3% NaOH solution to maintain the pH value of the reaction system to 8.3 during the reaction, and react at 43°C for 3.5h;

[0051] 3) After the reaction, wash with deionized water and ethanol in sequence, filter, and dry to obtain starch octenyl succinate, that is, the OSA starch sample, and set aside;

[0052]4) Take 0.1818g tellurium and 1.0908g potassium borohydride and react with 30mL water under nitrogen atmosphere to prepare sodium tellurium hydride sol...

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Abstract

The invention discloses a preparation method of telluride starch-based bionic glutathione peroxidase, which comprises the following steps: 1) taking cassava starch, adding water to prepare a starch emulsion, diluting octenyl succinic anhydride with ethanol, and adding the diluted octenyl succinic anhydride into the starch emulsion to react; 3) after the reaction is finished, sequentially washing with deionized water and ethanol, and performing filtering and drying to obtain starch octenylsuccinate; 4) reacting tellurium and potassium borohydride with water in a nitrogen atmosphere to prepare a sodium hydrogen telluride solution; 5) dispersing starch octenylsuccinate into absolute ethyl alcohol, and adding the sodium hydrogen telluride solution into the starch octenylsuccinate dispersion liquid in a nitrogen atmosphere for reaction; 6) after the reaction is finished, performing washing, carrying out suction filtration, and performing drying to obtain the starch octenylsuccinate telluride. According to the starch telluride-based bionic glutathione peroxidase prepared by the method disclosed by the invention, the raw materials are conventional materials and are relatively low in cost, and the prepared GPx stimulant is excellent in catalytic activity.

Description

technical field [0001] The invention belongs to the technical field of modified starch, and in particular relates to a preparation method of tellurized starch-based biomimetic glutathione peroxidase. Background technique [0002] Selenium is an essential micronutrient for plants, animals, including humans, and it affects the metabolism, antioxidant defense, and immune function of living cells. The "hidden hunger" caused by selenium deficiency is threatening human health. The production of agricultural products containing micronutrient selenium and other beneficial functional ingredients through food fortification technology is one of the important mitigation measures, and at the same time realize the nutritional and functionalization of agricultural products. [0003] GPx is an important selenium enzyme in the human body, which plays an extremely important role in anti-oxidation and has unique redox properties. It uses reduced glutathione (GSH) as a substrate, can catalyze ...

Claims

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Application Information

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IPC IPC(8): C08B31/04
Inventor 尹艳镇梁兴唐石成汪双双钟书明郑韵英张瑞瑞王锋胡汉娇焦淑菲刘子杰刘敏
Owner BEIBU GULF UNIV
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