Ganoderma lucidum few-spore variety with high polysaccharide yield and artificial cultivation method thereof

Abstract

The invention discloses a ganoderma lucidum few-spore variety with high polysaccharide yield and an artificial cultivation method thereof. The preservation number of the ganodorma lucidum M624 is GDMCC No: 60889. The artificial cultivation method comprises the steps of mycelium culture of lucid ganoderma M624, lucid ganoderma emergence management and fruiting body harvesting. According to the invention, the ganoderma lucidum M624 with high yield of polysaccharides is obtained by screening, in substitute cultivation, the content of crude polysaccharides in sporocarp reaches 2.40+ / -0.09% and is far higher than 0.9% required by pharmacopeia, and the content of crude polysaccharides in substitute cultivated ganoderma lucidum in the industry is generally 0.9-1.5%; moreover, the ganoderma lucidum M624 is low in sporulation quantity, the influence on a cultivation environment and a machine can be reduced, and the requirement for ganoderma lucidum cultivation with sporocarp as a harvesting target is met. The method can improve the crude polysaccharide content of the ganoderma lucidum raw material from the source, enrich the current market ganoderma lucidum variety, is also suitable for industrial large-scale production, avoids the influence of a large amount of spore powder generated in the ganoderma lucidum spore spraying period on the environment and machines, and meets the industrial development requirements of ganoderma lucidum.

Description

Technical field: [0001] The invention belongs to the technical field of agricultural microorganisms, and in particular relates to a high-yielding polysaccharide ganoderma oligospore variety and an artificial cultivation method thereof. Background technique: [0002] The efficacy of Ganoderma lucidum and its related products are well recognized at home and abroad. It was listed in the United States Pharmacopoeia as early as 2010. At present, the annual output value of its cultivation and processing industry (including health food, skin care products, etc.) has reached more than 100 billion yuan. There are 19 varieties of Ganoderma lucidum recognized in my country, including Hunong No. 1, one of which is imported from abroad, and three are obtained through protoplast fusion. However, according to the 2016 strain survey conducted by researchers at the Edible Fungi Research and Development Center of the Guangdong Provincial Institute of Microbiology, the main strains being used ...

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Problems solved by technology

According to literature reports, the polysaccharide content of Ganoderma lucidum varieties in the market is generally 0.9-1.5%, and a small part of Ganoderma lucidum cultivated by basswood can reach 1.8-2.5%. , substitute material cultivation is an inevitable trend of large-scale production of edible fungi

Moreover, according to domestic ganoderma cultivation enterprises, ganoderma species face the risk of degeneration after 1-2 years of use, not only in the decline in yield, but more importantly, the main components representing their effectiveness, such as ganoderma polysaccharides and ganoderma triterpenes, have dropped sharply. Seriously affect product quality, it can be seen that the breeding of Ganoderma lucidum varieties with high and stable yield and suitable for factory cultivation is very important and urgent

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