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Ganoderma lucidum few-spore variety with high polysaccharide yield and artificial cultivation method thereof

A technology of artificial cultivation and Ganoderma lucidum, applied in the field of agricultural microorganisms, can solve problems such as unfavorable sustainable development of cultivation production, great environmental damage, and impact on product quality, so as to facilitate processing and application, reduce the impact of cultivation environment and machines, and improve quality effect

Active Publication Date: 2021-04-16
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to literature reports, the polysaccharide content of Ganoderma lucidum varieties in the market is generally 0.9-1.5%, and a small part of Ganoderma lucidum cultivated by basswood can reach 1.8-2.5%. , substitute material cultivation is an inevitable trend of large-scale production of edible fungi
Moreover, according to domestic ganoderma cultivation enterprises, ganoderma species face the risk of degeneration after 1-2 years of use, not only in the decline in yield, but more importantly, the main components representing their effectiveness, such as ganoderma polysaccharides and ganoderma triterpenes, have dropped sharply. Seriously affect product quality, it can be seen that the breeding of Ganoderma lucidum varieties with high and stable yield and suitable for factory cultivation is very important and urgent

Method used

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  • Ganoderma lucidum few-spore variety with high polysaccharide yield and artificial cultivation method thereof
  • Ganoderma lucidum few-spore variety with high polysaccharide yield and artificial cultivation method thereof
  • Ganoderma lucidum few-spore variety with high polysaccharide yield and artificial cultivation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Example 1: Acquisition of Ganoderma Lucidum M624, a polysaccharide-yielding Ganoderma oligospore variety-Ganoderma lucidum M624

[0027] According to the long-term investigation of the resources of wild large fungi by the researchers of the Edible Fungi Research and Development Center of the Guangdong Provincial Institute of Microbiology, Ganoderma lucidum is mainly distributed in Guangdong, Hunan, Anhui, Henan and other places. Researchers from the Edible Fungi Research and Development Center of the Guangdong Provincial Institute of Microbiology collected wild The fruiting body of the strain HMGIM-M624 (namely ganoderma lucidum M624), the fruiting body has lateral stipe, the cap is flat and the diameter is about 2.8cm, and the length of the stipe is about 6.5cm; most of the cap is yellowish brown , the edge is light yellow and blunt, the surface of the hole is white, and the flesh is light brown ( figure 1 ).

[0028] 1. Tissue separation

[0029] Wipe the surface o...

Embodiment 2

[0042] Example 2: Domestication cultivation of Ganoderma lucidum M624 and evaluation of fruiting body crude polysaccharide content

[0043] 1. Domestication and cultivation of wild Ganoderma lucidum strains

[0044] (1) Culture medium formula and configuration

[0045] 1. Mother medium (enriched with comprehensive PDA): by mass fraction, including potato 20% + glucose 2% + peptone 1% + agar 2% + potassium dihydrogen phosphate 0.3% + magnesium sulfate 0.15% + vitamin B1 0.001 %, the balance is water, and its preparation method is: first wash and peel the potatoes, then take by weighing 200g potatoes and cut into small pieces, add water and boil until it is rotten (boil for 20-30 minutes, it can be pierced by a glass rod), Filter with eight layers of gauze, add 20g of glucose, 10g of peptone, 20g of agar, 3g of potassium dihydrogen phosphate, 1.5g of magnesium sulfate, and 0.01g of vitamin B1 to the filtrate. Sterilize at 121°C for 30 minutes under high temperature and high pr...

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Abstract

The invention discloses a ganoderma lucidum few-spore variety with high polysaccharide yield and an artificial cultivation method thereof. The preservation number of the ganodorma lucidum M624 is GDMCC No: 60889. The artificial cultivation method comprises the steps of mycelium culture of lucid ganoderma M624, lucid ganoderma emergence management and fruiting body harvesting. According to the invention, the ganoderma lucidum M624 with high yield of polysaccharides is obtained by screening, in substitute cultivation, the content of crude polysaccharides in sporocarp reaches 2.40+ / -0.09% and is far higher than 0.9% required by pharmacopeia, and the content of crude polysaccharides in substitute cultivated ganoderma lucidum in the industry is generally 0.9-1.5%; moreover, the ganoderma lucidum M624 is low in sporulation quantity, the influence on a cultivation environment and a machine can be reduced, and the requirement for ganoderma lucidum cultivation with sporocarp as a harvesting target is met. The method can improve the crude polysaccharide content of the ganoderma lucidum raw material from the source, enrich the current market ganoderma lucidum variety, is also suitable for industrial large-scale production, avoids the influence of a large amount of spore powder generated in the ganoderma lucidum spore spraying period on the environment and machines, and meets the industrial development requirements of ganoderma lucidum.

Description

Technical field: [0001] The invention belongs to the technical field of agricultural microorganisms, and in particular relates to a high-yielding polysaccharide ganoderma oligospore variety and an artificial cultivation method thereof. Background technique: [0002] The efficacy of Ganoderma lucidum and its related products are well recognized at home and abroad. It was listed in the United States Pharmacopoeia as early as 2010. At present, the annual output value of its cultivation and processing industry (including health food, skin care products, etc.) has reached more than 100 billion yuan. There are 19 varieties of Ganoderma lucidum recognized in my country, including Hunong No. 1, one of which is imported from abroad, and three are obtained through protoplast fusion. However, according to the 2016 strain survey conducted by researchers at the Edible Fungi Research and Development Center of the Guangdong Provincial Institute of Microbiology, the main strains being used ...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01G18/00C12R1/645
CPCA01G18/00C12N1/14C12R2001/645
Inventor 梁晓薇胡惠萍吴清平谢意珍刘远超肖春李向敏蔡曼君唐健寇秀颖
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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