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Burkholderiaspp.and application thereof in preventing and treating main pathogenic bacteria of panax notoginseng root rot

A Burkholderia and Panax notoginseng technology, applied to Burkholderia strains and its application in the prevention and treatment of the main pathogenic bacteria of Panax notoginseng root rot, can solve the problems of different genotypes, unsatisfactory effects, allergic reactions, etc., and achieve Enhance colonization, promote the formation of biofilm, and promote the effect of strain growth

Active Publication Date: 2021-04-13
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Root rot is caused by the imbalance of microorganisms in the soil, a large number of pathogens and fungi infecting the roots of Panax notoginseng plants. The imbalance of microorganisms in the soil is an important factor leading to continuous cropping obstacles of Panax notoginseng. Currently, the commonly used treatment methods are mainly the application of fungicides. However, the effect is becoming less and less ideal, and the method of using high-efficiency fumigants for soil killing treatment, and then supplementing probiotics to adjust the microbial flora to activate and strengthen the soil is relatively expensive and the effect is not ideal.
[0004] Burkholderia is a kind of Pseudomonas, which is widely distributed in the natural environment. Different strains have the same phenotype, but different genotypes. Some types of Burkholderia can be used in agriculture for biodegradation, biological control and Rhizosphere microorganisms that promote plant growth, but some are plant pathogenic bacteria and even human condition pathogenic bacteria, and because Burkholderia has strong antibiotic resistance and high motility, therefore, in agriculture The application of Burkholderia has certain potential risks, and the selection of strains needs to be very cautious
Chinese patent 201010290583.8 discloses a Burkholderia oryzae rhizosphere. This bacterial strain has a very good effect on the prevention and treatment of rice sheath blight, but when the bacterial suspension of this bacterial strain is used to inoculate tobacco leaves, it will cause typical symptoms within 24 hours. Allergic reactions, therefore, if Burkholderia is to be used to solve soil-borne diseases of different crops, how to isolate and purify the most suitable Burkholderia strain is very important

Method used

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  • Burkholderiaspp.and application thereof in preventing and treating main pathogenic bacteria of panax notoginseng root rot
  • Burkholderiaspp.and application thereof in preventing and treating main pathogenic bacteria of panax notoginseng root rot
  • Burkholderiaspp.and application thereof in preventing and treating main pathogenic bacteria of panax notoginseng root rot

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The isolation of embodiment 1 Burkholderia (Burkholderia) B36

[0017] A kind of Burkholderia (Burkholderiaspp.) B36 was isolated from the healthy rhizosphere soil of Panax notoginseng by plate separation method, and its morphology was identified. Shaped, yellow colonies, smooth and raised surface, irregular edges.

[0018] The preservation information of the Burkholderia spp. B36 is as follows: the preservation number is CGMCCNo.21179; the preservation unit is the General Microbiology Center of the China Committee for the Collection of Microorganisms; the preservation address is No. 1, Beichen West Road, Chaoyang District, Beijing 3 No., zip code: 100101; date of deposit is November 16, 2020.

Embodiment 2

[0019] Example 2 Burkholderia (Burkholderia) B36 to the main pathogenic bacteria rust rot and eggplant rot of Panax notoginseng root rot Inhibition of Fusarium

[0020] Fusarium solani and Cylindrocarpondestructans tested in this example were provided by the Plant Pathology Laboratory of Plant Protection College of Yunnan Agricultural University.

[0021] The specific method is as follows:

[0022] The inhibitory activity of Burkholderia B36 on the mycelial growth of the tested root rot fungus was determined by plate confrontation culture method. On the ultra-clean workbench, the Fusarium solani rot and the rust rot fungus grown on the PDA medium are used to make a bacterium cake with a 5mm puncher, and then the bacterium cake is transferred to the center of the PDA medium plate, Then use a sterile toothpick to inoculate the isolated and purified Burkholderia 2.5cm away from the pathogenic bacteria, set up 4 repetitions, take the plate with only pathogenic bacteria cakes ...

Embodiment 3

[0029] Example 3 Detection of Burkholderia B36 Degradability of Saponin Autotoxic Substances

[0030] Specific steps are as follows:

[0031] (1) Use an inoculation loop to pick a single purified Burkholderia B36 strain, inoculate it into 50 mL of NA liquid medium, place it on a shaker at 28°C and 150 r / min for overnight culture; then, take 100 μL of the bacterial liquid and add Into 200mL liquid medium with crude saponins of notoginseng as the sole carbon source (crude notoginseng saponins 1g / L), the treatment without adding bacterial solution was used as a blank control, and placed on a shaker for shaking culture (28°C, 150r / min ), each treatment was repeated three times, and samples were taken for measurement at 48h, 96h and 144h of culture.

[0032] Among them, the formula of NA medium is glucose 10g, peptone 5g, beef extract 3g, yeast extract 1g, agar powder 17g, water 1000mL.

[0033] (2) Take 100mL liquid (including the blank control) from each bottle of shaking cul...

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Abstract

The invention provides Burkholderiaspp.B36. The strain has relatively strong inhibitory activity on main root rot bacteria of panax notoginseng, namely rust rot bacteria, has relatively obvious degradation on main saponin autotoxins (R1, Rg1, Re, Rb1 and Rd) of panax notoginseng, and especially has significant degradation activity on saponins Rb1 and Rd. The Burkholderiaspp.B36 has a broad application prospect.

Description

technical field [0001] The invention relates to a bacterial strain, in particular to a Burkholderia strain and its application in preventing and treating the main pathogenic bacteria of Panax notoginseng root rot. Background technique [0002] Panax notoginseng is the main economic crop in Wenshan area of ​​Yunnan Province. It is warm and humid in nature, and its unique growth environment is easy to induce a variety of soil-borne diseases. Due to the magical medicinal effects of Panax notoginseng, the global demand for Panax notoginseng and its related products is increasing, but there are not many areas available for planting Panax notoginseng, so most areas adopt the continuous cropping method of Panax notoginseng. With the increase of Panax notoginseng planting years, the types, diseased area and severity of soil-borne diseases are increasing year by year, which seriously damages the yield and quality of Panax notoginseng. The most important disease is root rot. [0003]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/20A01P21/00A01P3/00C09K17/14C12R1/01C09K101/00
CPCA01N63/20C09K17/14C09K2101/00Y02A40/10
Inventor 朱书生杨敏邓琳梅王罗涛叶辰梅馨月刘屹湘黄惠川何霞红朱有勇
Owner YUNNAN AGRICULTURAL UNIVERSITY
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