Bacteria preparation for processing waste with high lignin content

A bacterial preparation and preparation technology, applied in the field of microorganisms, can solve the problems of reducing the quality of cellulose and hemicellulose, consuming chemical reagents, polluting the environment, etc., achieving broad application prospects and improving the effect of composting efficiency.

Active Publication Date: 2021-04-09
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the low conversion rate of lignin also limits the utilization of cellulose and hemicellulose
[0004] In the prior art, lignin is hardly utilized, and it is often removed from plants by using strong acid, strong alkali, etc., which not only consumes a lot of chemical reagents, but also pollutes the environment, and directly wastes a lot of lignin. And may reduce the quality of cellulose and hemicellulose
This also leads to poor treatment of garden waste, straw and other wastes with high lignin content.

Method used

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  • Bacteria preparation for processing waste with high lignin content
  • Bacteria preparation for processing waste with high lignin content
  • Bacteria preparation for processing waste with high lignin content

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1: Screening and identification of lignin-degrading bacteria

[0048] 1. Configuration of culture medium

[0049] (1) Alkali lignin medium: Alkali lignin 1.0g, NH 4 Cl 2.0g, K 2 HPO 4 1.0g, KH 2 PO 4 1.0g, MgSO 4 ·7H 2 O 0.20g, CaCl2 0.1g, FeSO 4 ·7H 2 O 0.05g, MnSO 4 ·7H 2 O 0.02g, add agar 15.0g, make up water to 1000mL. Sterilize at 121°C for 20 minutes. The corresponding liquid culture medium does not need to add agar.

[0050] (2) Potato medium (PDA): 200g of peeled potatoes, cut into pieces and boiled, filtered with gauze, added 20.0g of glucose to the filtrate, KH 2 PO 4 3.0g, MgSO 4 ·7H 2 O 1.5g, add agar 15.0g, make up water to 1000mL. Sterilize at 115°C for 30 minutes. The corresponding liquid culture medium does not need to add agar.

[0051] 2. Isolation and purification of strains

[0052] The composting plant corn stalk samples were inoculated on the alkali lignin solid medium under sterile conditions, and cultured at 30°C f...

Embodiment 2

[0065] Example 2: Optimization of conditions for degrading alkali lignin by lignin-degrading bacteria

[0066] Take the strains activated on the PDA slant for 24 hours, inoculate MSDA1 and HDGA2 in 100mL / 250mL Erlenmeyer flasks at 2% inoculum, and cultivate them at 30°C and 150r for 24 hours to prepare seed liquid. In the seed liquid, the concentration of viable bacteria of each microorganism ≥10 8 CFU / mL. Then the seed solution of each microorganism was inoculated into 50mL liquid alkali lignin medium according to the inoculation ratio of 10% by volume, and the single factor test was used to investigate different time, different nitrogen sources, different rotation speeds, different alkali lignin concentrations, etc. Effects on MSDA1 and HDGA2 degradation of alkali lignin. In addition to the investigated factors, the culture conditions are: pH=6.5, 30°C, 150r / min shaker culture for 5 days. details as follows:

[0067] 1. The effect of different time on the degradation rat...

Embodiment 3

[0084] Example 3: Validation of the effect of lignin-degrading bacteria on degrading lignin in sawdust

[0085] After drying the sawdust at 105°C, pass through a 40-mesh sieve, and then separate the PDA seed liquids of the fecal shell fungus MSDA1 and the hyphosporium HDGA2 (each viable bacteria concentration ≥ 10 8 CFU / mL) was inoculated into sawdust at an inoculum volume of 1 mL / g. After mixing thoroughly, the culture temperature was 30° C., and the culture was statically cultivated for 10 days. Inoculate MSDA1 directly into wood chips as group 1; add 3.4g / kg ammonium tartrate, and other conditions are the same as group 1, as group 2; directly inoculate HDGA2 into wood chips as group 3; add 1.5g / L ammonium nitrate, Other conditions are the same as group 3, as group 4. Determination of weight loss rate, degradation rate of lignin and cellulose (paradigm method) and selectivity coefficient. Selectivity coefficient = lignin degradation rate / cellulose degradation rate. The re...

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Abstract

The invention belongs to the field of microorganisms, and specifically relates to a bacteria preparation for processing waste with high lignin content. The specific technical schemes are as follows: thermomyces lanuginosus is deposited with the China General Microbiological Culture Collection Center on December 1st, 2020 and assigned accession number CGMCC No. 21075. The provided bacteria preparation includes the thermomyces lanuginosus and sordaria. Two novel microorganisms capable of degrading lignin are provided, have the capacity of independently degrading the lignin, and have a certain capacity of degrading cellulose and hemicellulose. Through the preparation of the two microorganisms into the bacteria preparation used for composting wastes with high lignin content, the effects of greatly enhancing composting efficiency can be achieved by only using the two microorganisms; and therefore, the bacteria preparation has wide application prospects on lignin processing.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a fungal preparation for treating waste with high lignin content. Background technique [0002] Lignin is a biomass that is second only to cellulose and chitin in nature, and it exists in various and irregular forms. According to the composition of lignin precursors, it can be divided into three categories: meson alcohol as a precursor, syringyl lignin (S-lignin) polymerized from syringyl phenylpropane structural monomers, coniferous lignin Alcohol as a precursor, guaiacyl lignin (Guajacyl lignin, G-lignin) polymerized from guaiacylphenylpropane structural monomer, coumaryl alcohol as a precursor from p-hydroxyphenylphenylpropane structural monomer p-Hydroxyphenyl lignin (H-lignin) formed by the polymerization of p-hydroxyphenyl lignin. [0003] At the same time, in different plants, the combination of structural units of lignin is different. The complex structure mak...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C05F11/00C05F11/08C05F17/20C12R1/645
CPCC12N1/14C05F11/00C05F11/08C05F17/20Y02W30/40
Inventor 李东刘金艳
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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