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Three-stage inoculation process and microbial agents for composting lignocelluloses raw material

A technology of lignocellulose and microbial inoculants, which is applied in the preparation of organic fertilizers, the treatment of biological organic parts, and organic fertilizers, can solve problems such as removal or destruction, improve efficiency, shorten composting time, and expand large-scale applications. Effect

Inactive Publication Date: 2012-04-25
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since lignin is generally wrapped around cellulose, the rapid and sufficient degradation of cellulose needs to remove or destroy lignin, although brown rot fungi can slowly degrade cellulose without destroying lignin, but in other cellulose degradation This ability has not been found in bacteria (such as Trichoderma, etc.)

Method used

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  • Three-stage inoculation process and microbial agents for composting lignocelluloses raw material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 The preparation of microbial inoculants used in three-stage composting, the specific steps are as follows:

[0020] (1) Preparation of high temperature bacteria

[0021] Using cow dung extract as the medium and 55°C culture conditions as the screening conditions, two thermophilic microorganisms that can grow normally on the cow dung extract medium under 55°C culture conditions were isolated from cow dung: Thermoactinomyces sp GF1 and Thermoactinomyces sp GF2.

[0022] The preparation of bacterial agents is divided into two stages. Specifically:

[0023] The first stage is fermentation. Inoculate the two strains on a plate containing PDA medium and place them in an incubator at 55°C for 5-7 days. After the two strains grow on the plate and produce spores, the spores are eluted and collected with sterile water. , Obtain the spore suspensions of the two strains respectively. The spore suspension of the two strains was centrifuged by a high-speed centrifuge at a c...

Embodiment 2

[0029] Example 2 Three-stage inoculation process of cow dung and straw compost

[0030] (1) Pretreatment of cow dung and straw

[0031] According to the research results of the optimized combination of high-temperature inoculants and culture conditions, the C / N ratio of the mixture of cow manure and straw was adjusted to 27, the moisture content was 75%, and the pH was 7.5.

[0032] (2) Preparation of microbial agents: the preparation method is as in Example 1.

[0033] (3) Stacking of raw materials, this stage is divided into the following three steps

[0034] In the first step, 360 ml of high-temperature inoculants are thoroughly mixed with 36.4 kg of cow dung and straw mixture, and the inoculum concentration reaches 1×10 per gram of piled material. 8 Pile up the mixed cow dung and straw, and monitor the temperature of the pile from the second day of stacking. The temperature of the stack reached 47°C on the second day, and reached the highest temperature of 68°C on the fourth day. ...

Embodiment 3

[0038] Example 3 Three-stage inoculation process of pig manure and straw compost

[0039] (1) Pretreatment of straw

[0040] According to the research results of the optimized combination of high-temperature inoculants and culture conditions, the C / N ratio of the straw mixture was adjusted to 25, the moisture content was 65%, and the pH was 6.

[0041] (2) Preparation of microbial agents: the preparation method is as in Example 1.

[0042] (3) Stacking of raw materials, this stage is divided into the following three steps

[0043] In the first step, 470 ml of high-temperature inoculum is thoroughly mixed with 47 kg of pig manure and straw mixture, and the inoculum concentration reaches 1×10 per gram of stock 8 Stack the mixed pig manure and straw, and monitor the temperature of the pile from the second day of stacking. The temperature of the stack reached 50°C on the second day and the maximum temperature of 73°C on the third day. The temperature of the stack was maintained at a high...

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Abstract

The invention discloses a three-stage inoculation process and microbial agents for composting a lignocellulose raw material. In three-stage inoculation, the microbial agents are a high temperature agent, a lignocellulose degrading agent and a cellulose degrading agent, the conventional one-time addition of the agents is changed into three-time addition, the high temperature agent is added in the early stage of piling to ensure that a pile body is subjected to high temperature and the influence of the high temperature on the lignocellulose degrading agent is avoided; the lignocellulose degrading agent is added in the later stage of high temperature and effectively degrades lignocellulose, and the lignocellulose is prevented from resisting cellulose degradation; and the cellulose degrading agent is added in the later stage of the action of the lignocellulose degrading agent to degrade cellulose. In the new three-stage composting fermentation inoculation process, the effects of the high temperature agent, the lignocellulose degrading agent and the cellulose degrading agent can be fully achieved, the composting efficiency is improved, the composting quality is improved, the composting period is shortened, and the process is a new process for composting the lignocellulose raw material.

Description

technical field [0001] The invention relates to a three-stage inoculation process for composting lignocellulosic raw materials and a microbial agent. technical background [0002] Although scholars at home and abroad have done a lot of work in the field of microbial agents for composting, the problems related to microbial inoculation and composting research are debatable. One is the effect of high temperature on the inoculum in composting. In all researches on inoculants, almost all the methods of mixing and one-time dosing of microbial inoculants are adopted, such as Chen Furong et al. (2): 84-87) The 5 species of bacteria screened from agricultural and forestry waste compost, according to the number ratio of bacteria:actinomycetes:fungus is 85:5:10, Bacillus subtilis:Pseudomonas aeruginosa is 55: 25. Aspergillus niger: Penicillium simplex in a 2:1 ratio to make bacterial agents. However, the rationality of this all-mixing and one-time dosing of microbial agents is quest...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F17/00
CPCY02W30/40
Inventor 周成宁平刘张黄兆林许菲
Owner KUNMING UNIV OF SCI & TECH
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