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Antioxidant function of corn heat shock transcription factor gene ZmHsf17 encoding protein and application thereof

A heat shock transcription factor, gene technology, applied in the field of genetic engineering, can solve problems such as lack of

Inactive Publication Date: 2021-04-09
HEBEI ACADEMY OF AGRI & FORESTRY SCI INST OF GENETICS & PHYSIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is a lack of gene resources with strong anti-oxidation functions and clear functions, and there are fewer genes that improve the overall stress resistance of plants through anti-oxidation

Method used

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  • Antioxidant function of corn heat shock transcription factor gene ZmHsf17 encoding protein and application thereof
  • Antioxidant function of corn heat shock transcription factor gene ZmHsf17 encoding protein and application thereof
  • Antioxidant function of corn heat shock transcription factor gene ZmHsf17 encoding protein and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] This embodiment provides the preparation process of the maize heat shock transcription factor gene ZmHsf17:

[0055] (1) Exogenous H 2 o 2 Handling corn seedlings

[0056] The test material is maize (Zea mays L.) inbred line H21 (prepared in the laboratory). The selected seeds were disinfected on the surface, rinsed repeatedly with tap water, soaked and imbibed for 12 hours, then germinated in an incubator at 28°C, and sowed in Hoagland nutrient solution when the root length was 1-2 cm. When the corn seedlings grow to two leaves and one core, soak the root system in a solution containing 10mmol / LH 2 o 2 treated in the Hoagland solution, and the roots and first expanded leaves were taken at 0, 15, 30, 60, 90, 120, 240 and 480 min, respectively, and were quick-frozen in liquid nitrogen for quantitative analysis of ZmHsf17 gene.

[0057] (2) RNA extraction

[0058] The frozen roots and leaves were put into a mortar and ground quickly until the plant tissue was powder...

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Abstract

The invention discloses an antioxidant function of a corn heat shock transcription factor gene ZmHsf17 encoding protein and application thereof, and belongs to the field of genetic engineering. The nucleotide sequence of the corn heat shock transcription factor gene ZmHsf17 is shown as SEQ ID NO. 1, the gene ZmHsf17 is obtained by cloning from a corn (Zea mays L.) inbred line H21, and can activate the expression of a plurality of downstream stress-resistant functional genes, and the gene can significantly improve the antioxidant function of plants by transforming arabidopsis. The corn heat shock transcription factor encoding gene of the invention will provide a backup gene resource for crop stress resistance genetic improvement and germplasm innovation.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to the anti-oxidation function of a protein encoded by a maize heat shock transcription factor gene ZmHsf17 and its application. Background technique [0002] With the intensification of the global greenhouse effect, climate warming has posed severe challenges to modern agricultural production, and high temperature is often accompanied by drought, and the immobility of plants leads to continuous exposure to adversity stress environments. In order to adapt to the deteriorating atmospheric environment, plants have gradually evolved a response network mechanism to cope with stress, and heat shock transcription factor (Hsf) is an important class of functional genes in this regulatory network. The Hsf family is the terminal regulatory element located in the heat stress conduction chain, which can directly activate the expression of heat stress and other stress res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/84A01H5/00A01H6/20
CPCC07K14/415C12N15/8271
Inventor 郭秀林张华宁李国良刘子会张红梅段硕楠
Owner HEBEI ACADEMY OF AGRI & FORESTRY SCI INST OF GENETICS & PHYSIOLOGY
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