Preparation method of liquid woundplast based on stem cell culture supernatant
A technology of stem cell culture and culture supernatant, which is applied in the field of preparation of liquid band-aids, can solve the problems of cytokine denaturation, inactivation, and inactivation, and achieve the effects of easier absorption, preventing denaturation and inactivation, and preventing changes in biological characteristics
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Embodiment 1
[0021] A liquid band-aid based on stem cell culture supernatant, including film-forming liquid, active substance and solvent, said film-forming liquid includes chitosan hydrochloride, polyvinylpyrrolidone, hyaluronic acid and chondroitin sulfate, said chitosan 2-10 parts of sugar hydrochloride, 0.5-5 parts of polyvinylpyrrolidone, 0.05-5 parts of hyaluronic acid and 0.05-5 parts of chondroitin sulfate, the active substance is the freeze-dried powder of stem cell culture supernatant, and the solvent is sterile water.
[0022] The preparation method of described film-forming liquid, comprises the steps:
[0023] 1) Weigh 6-10 parts of chitosan hydrochloride and add it to 50 parts of centrifuge tubes, add 50 parts of sterile water to fully dissolve it to obtain chitosan hydrochloride solution; add 0.5-2 parts of polyvinylpyrrolidone Dissolved in 20 parts of sterile water to obtain polyvinylpyrrolidone aqueous solution;
[0024] 2) Mix the two solutions in step 1), place in a co...
Embodiment 2
[0034] A liquid band-aid based on stem cell culture supernatant, including film-forming liquid, active substance and solvent, said film-forming liquid includes chitosan hydrochloride, polyvinylpyrrolidone, hyaluronic acid and chondroitin sulfate, said chitosan 4 parts of sugar hydrochloride, 1.1 parts of polyvinylpyrrolidone, 0.06 parts of hyaluronic acid and 4.5 parts of chondroitin sulfate, the active substance is freeze-dried powder of stem cell culture supernatant, and the solvent is sterile water.
[0035] The preparation method of described film-forming liquid, comprises the steps:
[0036]1) Weigh 4 parts of chitosan hydrochloride and add it to 50 parts of centrifuge tubes, add 50 parts of sterile water to fully dissolve it to obtain chitosan hydrochloride solution; dissolve 1.1 parts of polyvinylpyrrolidone in 20 parts Obtain polyvinylpyrrolidone aqueous solution in sterile water;
[0037] 2) Mix the two solutions in step 1), place in a constant temperature water bath...
Embodiment 3
[0047] A liquid band-aid based on stem cell culture supernatant, including film-forming liquid, active substance and solvent, said film-forming liquid includes chitosan hydrochloride, polyvinylpyrrolidone, hyaluronic acid and chondroitin sulfate, said chitosan 8 parts of sugar hydrochloride, 2 parts of polyvinylpyrrolidone, 0.05 parts of hyaluronic acid and 5 parts of chondroitin sulfate, the active substance is freeze-dried powder of stem cell culture supernatant, and the solvent is sterile water.
[0048] The preparation method of described film-forming liquid, comprises the steps:
[0049] 1) Weigh 8 parts of chitosan hydrochloride and add it to 50 parts of centrifuge tubes, add 50 parts of sterile water to fully dissolve it to obtain chitosan hydrochloride solution; dissolve 2 parts of polyvinylpyrrolidone in 20 parts Obtain polyvinylpyrrolidone aqueous solution in sterile water;
[0050] 2) Mix the two solutions in step 1), place in a constant temperature water bath at 4...
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