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Preparation method of liquid woundplast based on stem cell culture supernatant

A technology of stem cell culture and culture supernatant, which is applied in the field of preparation of liquid band-aids, can solve the problems of cytokine denaturation, inactivation, and inactivation, and achieve the effects of easier absorption, preventing denaturation and inactivation, and preventing changes in biological characteristics

Inactive Publication Date: 2021-04-09
海南优尼科尔生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there will be several technical problems in adding cytokines to dressings: (1) how to keep the activity of each active factor in the stem cell culture supernatant at a high level during use and storage; (2) the film-forming factor currently used in liquid dressings The components are all based on organic solvents, which can easily cause the denaturation of cytokines and cause them to lose their activity. How to ensure the activity of cytokines and at the same time ensure the fast film formation speed; factor inactivating

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A liquid band-aid based on stem cell culture supernatant, including film-forming liquid, active substance and solvent, said film-forming liquid includes chitosan hydrochloride, polyvinylpyrrolidone, hyaluronic acid and chondroitin sulfate, said chitosan 2-10 parts of sugar hydrochloride, 0.5-5 parts of polyvinylpyrrolidone, 0.05-5 parts of hyaluronic acid and 0.05-5 parts of chondroitin sulfate, the active substance is the freeze-dried powder of stem cell culture supernatant, and the solvent is sterile water.

[0022] The preparation method of described film-forming liquid, comprises the steps:

[0023] 1) Weigh 6-10 parts of chitosan hydrochloride and add it to 50 parts of centrifuge tubes, add 50 parts of sterile water to fully dissolve it to obtain chitosan hydrochloride solution; add 0.5-2 parts of polyvinylpyrrolidone Dissolved in 20 parts of sterile water to obtain polyvinylpyrrolidone aqueous solution;

[0024] 2) Mix the two solutions in step 1), place in a co...

Embodiment 2

[0034] A liquid band-aid based on stem cell culture supernatant, including film-forming liquid, active substance and solvent, said film-forming liquid includes chitosan hydrochloride, polyvinylpyrrolidone, hyaluronic acid and chondroitin sulfate, said chitosan 4 parts of sugar hydrochloride, 1.1 parts of polyvinylpyrrolidone, 0.06 parts of hyaluronic acid and 4.5 parts of chondroitin sulfate, the active substance is freeze-dried powder of stem cell culture supernatant, and the solvent is sterile water.

[0035] The preparation method of described film-forming liquid, comprises the steps:

[0036]1) Weigh 4 parts of chitosan hydrochloride and add it to 50 parts of centrifuge tubes, add 50 parts of sterile water to fully dissolve it to obtain chitosan hydrochloride solution; dissolve 1.1 parts of polyvinylpyrrolidone in 20 parts Obtain polyvinylpyrrolidone aqueous solution in sterile water;

[0037] 2) Mix the two solutions in step 1), place in a constant temperature water bath...

Embodiment 3

[0047] A liquid band-aid based on stem cell culture supernatant, including film-forming liquid, active substance and solvent, said film-forming liquid includes chitosan hydrochloride, polyvinylpyrrolidone, hyaluronic acid and chondroitin sulfate, said chitosan 8 parts of sugar hydrochloride, 2 parts of polyvinylpyrrolidone, 0.05 parts of hyaluronic acid and 5 parts of chondroitin sulfate, the active substance is freeze-dried powder of stem cell culture supernatant, and the solvent is sterile water.

[0048] The preparation method of described film-forming liquid, comprises the steps:

[0049] 1) Weigh 8 parts of chitosan hydrochloride and add it to 50 parts of centrifuge tubes, add 50 parts of sterile water to fully dissolve it to obtain chitosan hydrochloride solution; dissolve 2 parts of polyvinylpyrrolidone in 20 parts Obtain polyvinylpyrrolidone aqueous solution in sterile water;

[0050] 2) Mix the two solutions in step 1), place in a constant temperature water bath at 4...

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PUM

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Abstract

The invention discloses a preparation method of a liquid woundplast based on stem cell culture supernatant. The liquid woundplast comprises a film-forming solution, an active substance and a solvent; the film-forming solution comprises 2-10 parts of chitosan hydrochloride, 0.5-5 part of polyvinylpyrrolidone, 0.05-5 part of hyaluronic acid and 0.05-5 part of chondroitin sulfate; the active substance is self-made stem cell culture supernatant freeze-dried powder; and the solvent is sterile water. The liquid woundplast belongs to the technical field of medical dressings. The liquid woundplast based on stem cell culture supernatant can quickly form a film and slowly release active substances, and has the functions of continuously and efficiently repairing wounds and inhibiting scar formation.

Description

technical field [0001] The invention belongs to the technical field of medical dressings, in particular to a method for preparing a liquid band-aid based on stem cell culture supernatant. Background technique [0002] Wound healing is a complex process involving re-epithelialization of skin defects, hyperplasia of granulation tissue, inflammatory response, angiogenesis, and wound remodeling. Any problem in any of these processes will lead to impaired wound healing. A large number of studies have shown that cytokines play an important role in all stages of wound healing. This is due to their unique ability to stimulate quiescent cells to continue to undergo mitosis in vitro, and they can affect various stages of wound healing. This speeds up healing. Various factors are known to be involved in the tissue damage repair process, including chemokines, interleukins, growth factors, etc. Among them, interleukin 6 (IL-6), interleukin 8 (IL-8), monocyte chemoattractant protein 1 (...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L26/00
CPCA61L26/0057A61L26/0066A61L26/0023A61L26/0014A61L26/0061A61L26/0047A61L2300/412A61L2300/602C08L5/08C08L39/06C08L89/00
Inventor 王晓宇李崴郭康合林冠妃王岱桑张万程吴清毅
Owner 海南优尼科尔生物科技有限公司
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