A method for establishing a moon persimmon in vitro regeneration system

Abstract

The invention discloses a method for establishing a persimmon in vitro regeneration system, and relates to the technical field of plant cultivation. The key points of the technical scheme are: the primary culture is inoculated into the basic medium of DKW+ZT 0.5-3.0 mg / L and cultivated for 20-30 days; Subculture was inoculated into the basic medium of DKW+ZT 0.5~3.0mg / L+IAA 0.05~0.5mg / L for culture; callus induction was inoculated in 1 / 2DWK+ZT 0.5~3.0mg / L+TDZ 0.25 After 20-35 days of dark culture in ~2.0mg / L basic medium, the formation of callus and adventitious buds was induced under light; adventitious bud regeneration was inoculated in 1 / 2DKW+ZT 0.5-4.0mg / L and differentiated for 25-40 days ; Inoculate in the basic medium of 1 / 2DKW+IAA 0.5-4.0 mg / L, culture in the dark for 5-10 days, and then culture in the light for 20-35 days. The invention can efficiently and stably complete the in vitro regeneration and cultivation of the astringent persimmon, has high reproduction rate, high quality and short cultivation time, and lays a foundation for the genetic improvement of the astringent persimmon through genetic engineering.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, and more specifically relates to a method for establishing a persimmon in vitro regeneration system. Background technique [0002] Persimmon belongs to Diospyros Linn. of the persimmon family (Ebenaceae). As a representative species of fruit tree cultivation, it has been cultivated in my country for more than 2,000 years. Persimmon is rich in nutrition. The fresh fruit is bright in color, sweet in taste and rich in nutrients. The persimmon fruit is also very easy to process. It can be used to make persimmon cakes, dried persimmons, persimmon tea, persimmon vinegar and persimmon wine. It can also replace food, and is as famous as chestnut and jujube. It is known as "hardcore crop" and "woody food". Many components of persimmon can be used as medicine, including persimmon cream, persimmon pedicle, persimmon root, persimmon leaf, which can be used to hangover and lower blood pressure. In...

AI Technical Summary

Problems solved by technology

[0004] At present, firstly, for the primary cultivation of persimmons, most researches often select the twigs drawn in the same year in order to avoid the pollution of endophytic bacteria when collecting materials, generally 3 to 5 cm long. Bacteria are easily killed, and the stem segment is too short, the internode distance is small, and the buds are dense, which is not conducive to the germination and growth of buds on the stem segment.

In the second aspect, different plant growth regulators and concentrations have a greater impact on the primary culture of persimmons, among which BA and ZT are the most common. Existing studies have shown that many persimmon species cannot grow normally in the medium containing BA, and BA can Induces polyphenol oxidase activity, which can easily lead to browning of the base of the first generation seedlings and increase mortality

In the fourth aspect, existing studies have shown that excessive ZT concentration in the subculture of persimmon can easily lead to the formation and overgrowth of callus at the base of the stem, thereby inhibiting the germination of axillary buds and hindering the growth of leaves.

[0005] In summary, the existing primary culture and subculture of persimmon have many limitations, and cannot be directly applied to the process of genetic modification of astringent persimmon under the premise of maintaining other excellent traits through plant genetic engineering technology.

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