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Method for catalytic regeneration of NAD(P)H by supported metal catalyst

A metal catalyst and supported technology, which is applied in the field of supported metal catalyst catalytic regeneration of NADH, can solve the problems of low coenzyme regeneration efficiency and long reaction time, and achieve the effect of eliminating metal toxicity, easy operation and mild reaction conditions

Active Publication Date: 2021-04-06
BEIJING UNIV OF CHEM TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, what is currently involved is the regeneration of coenzymes by supported single-metal nanocatalysts. The regeneration efficiency of such supported single-metal catalysts for coenzymes is not high, and the reaction time is long. method has not yet been reported

Method used

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  • Method for catalytic regeneration of NAD(P)H by supported metal catalyst
  • Method for catalytic regeneration of NAD(P)H by supported metal catalyst
  • Method for catalytic regeneration of NAD(P)H by supported metal catalyst

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Experimental program
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Embodiment approach

[0048] As mentioned earlier, the existing NAD(P)H regeneration process is not satisfactory, and there are some problems in one way or another. For example, some NAD(P)H regeneration processes require toxic and expensive electron mediators ([Cp* Rh(bpy)H 2 O] 2+ ) participation and poor selectivity; some NAD(P)H regeneration processes have low regeneration efficiency and long reaction time; in view of this, the present inventors have conducted a large amount of research on NAD(P)H regeneration processes.

[0049] The present inventors have found that a toxic electron mediator ([Cp*Rh(bpy)H 2 O] 2+ ), the regenerated coenzyme has electron transfer activity detected by the MTT method. The method has the advantages of mild reaction conditions, high coenzyme regeneration efficiency, rapid reaction, high selectivity, environmental friendliness, simple and easy operation, etc.; thereby obtaining the present invention.

[0050] Therefore, the method for the catalyzed regeneration ...

Embodiment 1

[0083] Embodiment 1: Supported bimetallic nanocatalyst and supported single metal nanocatalyst to NADP + reduction

[0084] Contain 0.5mmol / L oxidized coenzyme NADP in 2mL + Add 5mg of supported metal catalyst to the buffer solution of 200mmol / L triethanolamine, so that the working concentration of the catalyst is 2.5mg / mL, control the temperature at 25°C, stir at 600rpm for 240min, and use the 340nm ultraviolet absorption method to detect the reduced coenzyme NADPH in the reaction system The concentration of supported bimetallic nanocatalysts and supported monometallic nanocatalysts on NADP + The restored comparison results are as follows figure 1 shown.

[0085] Compared figure 1 Supported bimetallic nanocatalysts and supported monometallic nanocatalysts in NADP + The restoration results can be seen. The regeneration activity of supported monometallic catalyst copper and gold to NADPH is very low, and the regeneration efficiency of monometallic palladium to NADPH can r...

Embodiment 2

[0086] Embodiment 2: 340nm ultraviolet absorption method and the NAD(P)H concentration of the supported bimetal nanocatalyst regeneration that MTT method detects

[0087] Contain 0.5mmol / L oxidized coenzyme NAD(P) in 2mL + Add 5mg of supported gold-palladium bimetallic nanocatalyst to the buffer solution of 200mmol / L triethanolamine, so that the working concentration of the catalyst is 2.5mg / mL, control the temperature at 25°C, stir at 600rpm for 240min, and use the 340nm ultraviolet absorption method to detect the reaction system The total amount of the reduced coenzyme NAD(P)H in the medium; the concentration of 1,4-NAD(P)H with electron transfer activity in the reaction system was detected by the MTT method, and the results were as follows figure 2 shown.

[0088] Compared figure 2 The NAD(P)H results of the regeneration of the supported bimetallic nanocatalysts detected by the 340nm ultraviolet absorption method and the MTT method show that the supported gold-palladium...

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Abstract

The invention relates to a method for catalytic regeneration of NAD(P)H by a supported metal catalyst, which comprises the following steps: reducing oxidized coenzyme under the action of the supported metal catalyst to obtain reduced coenzyme NAD(P)H; wherein the supported metal catalyst is a supported bimetallic nano catalyst; The coenzyme regenerated by the method disclosed by the invention has the electron transfer activity of the coenzyme after being tested by an MTT (Methyl Thiazolyl Tetrazolium) method. The method for catalytically regenerating the NAD (P) H by the supported metal catalyst has the advantages of mild reaction conditions, high efficiency, simplicity and convenience in operation, high selectivity, environmental friendliness and the like.

Description

technical field [0001] The invention belongs to the fields of biochemical catalysis and biomanufacturing, and in particular relates to a method for catalytically regenerating NAD(P)H with a supported metal catalyst. Background technique [0002] Biocatalysis has the advantages of mild reaction conditions, high catalytic efficiency and high selectivity, and is widely used in the chemical and pharmaceutical industries including daily chemicals and high value-added pharmaceutical production (Straathof, Adrie J J. Chemical Reviews, 2014, 114 (3): 1871-908; Bornscheuer U T, Huisman G W, Kazlauskas R J, et al. Nature, 2012, 485(7397): 185-194.). Oxidoreductases are the largest group of enzymes (accounting for about 25% of all enzymes) involved in biocatalytic processes. Oxidoreductases have a wide range of fields such as double bond oxidation, redox of aldehydes and organic acids, biological nitrogen fixation and biological hydrogen production. (Wu H, Tian C, Song X, et al. Green...

Claims

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Application Information

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IPC IPC(8): C07H1/00C07H19/207B01J23/52B01J31/40B01J38/00B01J38/48B01J38/52
CPCC07H1/00C07H19/207B01J23/52B01J31/40B01J38/48B01J38/52B01J38/00Y02P20/584
Inventor 苏海佳王耀强肖刚
Owner BEIJING UNIV OF CHEM TECH
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