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A cesium-137 mutation breeding method for macadamia nuts

A technology for mutation breeding and nut, applied in the fields of botanical equipment and methods, plant genetic improvement, application, etc., can solve problems such as single method

Active Publication Date: 2022-02-22
SOUTH ASIAN TROPICAL AGRI SCI RES INST OF GUANGXI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, macadamia nuts are usually bred by hybrid breeding, which is a single method, and it is of high research value to find breeding methods other than hybrid breeding

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A cesium-137 mutation breeding method of macadamia nuts, comprising the following steps:

[0023] (1) Seed selection: Select mature shelled macadamia nuts with a diameter of 25㎜~30㎜, a kernel moisture content of 15.5%~20.0%, and a smooth and undamaged shell surface as seeds; the seeds can be picked in the same year, or It can be the seeds picked in the previous year, and it is not suitable to use seeds that have been stored for more than two years; in this implementation, the seeds picked in the current year are used, a total of 100;

[0024] (2) Seed pre-treatment: put the seeds into the sterilizing solution for 3 hours, and filter the water to obtain sterilized seeds; soak the sterilized seeds in the pre-treatment solution for 4 hours, and dry them in the shade to obtain the pre-treated seeds. Seeds; the pretreatment liquid uses water as a solvent, and contains ethanol with a concentration of 50g / kg, citric acid with a concentration of 20g / kg and sodium chloride with ...

Embodiment 2

[0033] A cesium-137 mutation breeding method of macadamia nuts, comprising the following steps:

[0034] (1) Seed selection: Select mature shelled macadamia nuts with a diameter of 25㎜~30㎜, a kernel moisture content of 15.5%~20.0%, and a smooth and undamaged shell surface as seeds; the seeds can be picked in the same year, or It can be the seeds picked in the previous year, and it is not suitable to use seeds that have been stored for more than two years; in this implementation, the seeds picked in the current year are used, a total of 100;

[0035] (2) Seed pre-treatment: put the seeds in the sterilizing solution for 5 hours, and filter the water with a filter to obtain sterilized seeds; put the sterilized seeds in the pre-treatment solution for 6 hours, and dry them in the shade to obtain the pre-treated seeds. Seeds; the pretreatment liquid uses water as a solvent, containing ethanol with a concentration of 100g / kg, citric acid with a concentration of 25g / kg and sodium chlo...

Embodiment 3

[0044] A cesium-137 mutation breeding method of macadamia nuts, comprising the following steps:

[0045] (1) Seed selection: Select mature shelled macadamia nuts with a diameter of 25㎜~30㎜, a kernel moisture content of 15.5%~20.0%, and a smooth and undamaged shell surface as seeds; the seeds can be picked in the same year, or It can be the seeds picked in the previous year, and it is not suitable to use seeds that have been stored for more than two years; in this implementation, the seeds picked in the current year are used, a total of 100;

[0046] (2) Seed pre-treatment: put the seeds into the sterilizing solution for 4 hours, and filter the water to obtain sterilized seeds; soak the sterilized seeds in the pre-treatment solution for 5 hours, and dry them in the shade to obtain the pre-treated seeds. Seed; the pretreatment liquid uses water as a solvent, and contains ethanol with a concentration of 80g / kg, citric acid with a concentration of 23g / kg and sodium chloride with a...

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PUM

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Abstract

The invention discloses a cesium-137 mutation breeding method for macadamia nuts. The macadamia nut seeds are sterilized first, which can kill the disease bacteria on the upper surface of the macadamia nut seeds, reduce the spread of diseases, and prevent the occurrence of field diseases; put the sterilized seeds into Soaking treatment in the pre-treatment solution can strengthen the effect of chemical mutagenesis and promote the chemical mutagenesis of macadamia nut seeds; the chemical mutagen is a mixture of ethyl methanesulfonate and diethyl sulfate, which can accelerate the mutagenesis of macadamia nuts. Seed mutation speed, increase the mutation rate, and can obtain seeds with special traits; also limit the treatment process of chemical mutagenesis, which is divided into two mutagenesis treatments, so that the effect of mutagenesis is better; adopt chemical mutagenesis treatment and 137 The combination of Cs-γ-ray irradiation treatment can further accelerate the mutation speed of macadamia nut seeds, increase the mutation rate, and can obtain seeds with special traits; the irradiated seeds are subjected to shock treatment, which can further accelerate the mutation speed of macadamia nut seeds and improve mutation rate.

Description

technical field [0001] The invention relates to the technical field of agricultural planting, in particular to a cesium-137 mutation breeding method for macadamia nuts. Background technique [0002] Macadamia nut alias: Queensland chestnut, macadamia walnut, macadamia nut, Queensland fruit, is a tree nut native to Australia. Macadamia nuts are evergreen trees and dicotyledonous plants. The crown is tall, with 3 to 4 leaves in whorls, lanceolate, leathery, smooth, with spiny serrations on the edges. Racemes axillary, beige flowers, spherical fruit, leathery, hard endocarp, beige to light brown kernels. It is suitable for growing in mild, humid and windy areas. Among the many dried fruits in the world, macadamia nuts have the highest economic value and have always enjoyed the reputation of "the king of dried fruits". The kernel of macadamia nuts is rich in nutrition (milky white), the exocarp is turquoise, the endocarp is hard and brown, the weight of a single fruit is 15-...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/06
CPCA01H1/06
Inventor 谭秋锦王文林宋海云韦媛荣汤秀华何铣扬郑树芳张涛韦哲君
Owner SOUTH ASIAN TROPICAL AGRI SCI RES INST OF GUANGXI
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