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Bioactive peptide ASEPPVLDVKRPFLC as well as preparation method and application thereof

A bioactive peptide and active technology, applied in the field of protein, to achieve the effect of improving quality, improving function and improving ability

Active Publication Date: 2021-04-02
ZHEJIANG PANDA DAIRY GRP COMPANY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research on the function of Cytochrome b-c1 complex subunit Rieske, mitochondrial protein polypeptide fragments in the prior art

Method used

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  • Bioactive peptide ASEPPVLDVKRPFLC as well as preparation method and application thereof
  • Bioactive peptide ASEPPVLDVKRPFLC as well as preparation method and application thereof
  • Bioactive peptide ASEPPVLDVKRPFLC as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The artificial synthesis of embodiment 1 active peptide ASEPPVLDVKRPFLC

[0033] 1. Synthesis of Bioactive Peptides

[0034] 1. Weigh 3g of RINK resin (degree of substitution: 0.3mmol / g) into a 150ml reactor and soak in 50ml of dichloromethane (DCM).

[0035] 2. After 2 hours, wash the resin with nitrogen-dimethylformamide (DMF) that is 3 times the volume of the resin, and then drain it. Repeat this four times, and drain the resin for use.

[0036] 3. Add a certain amount of 20% piperidine (piperidine / DMF=1:4, v:v) into the reactor, and shake it on a decolorizing shaker for 20 minutes to remove the Fmoc protecting group on the resin. After deprotection, wash four times with 3 times the resin volume of DMF, and then drain.

[0037] 4. Take a small amount of resin and use ninhydrin (Nine Wells Ninhydrin) method to test it (Two drops each of Test A and Test B, react at 100°C for 1 min), if the resin is colored, it means that the deprotection is successful.

[0038] 5. W...

Embodiment 2

[0075] The immune activity experiment of embodiment 2 bioactive peptides

[0076] 1. The experiment of bioactive peptide ASEPPVLDVKRPFLC promoting macrophages to secrete cytokines (ELISA method)

[0077] 1. Experimental reagents and instruments:

[0078] Reagents: Experimental animal balb / c mice (male 6, 8 weeks old), Shanghai Slack Experimental Animal Co., Ltd.; mouse lymphocyte extract, Shanghai Suo Laibao Biotechnology Co., Ltd.; RPMI1640 medium, GIBCO Company; cattle Serum albumin (bovine serum albumin, BSA), Genebase Company; mouse spleen lymphocyte-derived bioactive peptide ASEPPVLDVKRPFLC obtained in Example 1; ELISA cytokine rapid kit (TNF-α and IL-1β), Wuhan Boster Bioengineering Ltd.

[0079] Equipment: LRH, 250F biochemical incubator Shanghai Heng Technology Co., Ltd.; GL, 22M high-speed refrigerated centrifuge Shanghai Luxiangyi Centrifuge Instrument Co., Ltd.; Hera cell 150CO 2 Incubator Heraeus Company; Dragon WellscanMK3 microplate reader Labsystems Company. ...

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Abstract

The invention relates to the field of protein, in particular to bioactive peptide ASEPPVLDVKRPFLC (Adenosine Sulfate-Polyvinylpyrrolidone-Vitamin K Receptor Fluorescent Cell) as well as a preparationmethod and application thereof. The amino acid sequence of the bioactive peptide ASEPPVLDVKRPFLC is Ala-Ser-Glu-Pro-Pro-Val-Leu-Asp-Val-Lys-Arg-Pro-Phe-Leu-Cys. An in-vitro immune function regulationexperiment proves that the bioactive peptide ASEPPVLDVKRPFLC has a relatively good immune regulation function. The bioactive peptide ASEPPVLDVKRPFLC can effectively inhibit inflammation caused by oxidation of a body, promote macrophages to secrete cytokines, improve the effect of the macrophages in a resting state of normal macrophages, improve the capacity of the body for resisting external pathogen infection, reduce the morbidity of the body and improve the life quality, and has very important significance in developing food, health care products and medicines with immunoregulation functions.

Description

technical field [0001] The invention relates to the field of proteins, in particular to a biologically active peptide ASEPPVLDVKRPFLC and its preparation method and application. Background technique [0002] In recent years, bioactive peptides have become a familiar term. Because of its many potential biological functions, it has attracted more and more attention and has become one of the hot spots of scientific research. At present, the beneficial effects of many bioactive peptides have also been well proven, such as anti-cancer, lowering blood pressure, antibacterial, lowering cholesterol, anti-diabetic and other properties. More than 3000 different bioactive peptides have been reported in BIOPEP-UMW, the most authoritative bioactive peptide database. [0003] At present, most studies on bioactive peptides focus on food-derived peptides, while there are few studies and reports on non-food-derived peptides. And it has been confirmed by research results that, compared wit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/00A61K8/66A61K38/43A61Q19/00A61P37/02A61P29/00A23L33/18
CPCC12N9/00A61K8/66A61P29/00A61P37/02A61Q19/00A23L33/18A61K38/00A23V2002/00A23V2200/324
Inventor 张少辉占文静陈承余肖珊珊石毅洪志骏王娟李锡安梁世排梁世赞
Owner ZHEJIANG PANDA DAIRY GRP COMPANY
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