iRGD magnetic targeting microbubble contrast agent and application thereof

A technology targeting microbubbles and contrast agents, applied in general/multifunctional contrast agents, microcapsules, echo/ultrasonic imaging agents, etc., to achieve good biocompatibility, reduce magnetic resonance signals, and facilitate observation

Active Publication Date: 2021-04-02
GUANGDONG NO 2 PROVINCIAL PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the combination of tumor penetrating peptide iRGD with ferric oxide nanoparticles (Fe 3 o 4 ) targeting new microvessels has not yet been reported

Method used

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  • iRGD magnetic targeting microbubble contrast agent and application thereof
  • iRGD magnetic targeting microbubble contrast agent and application thereof
  • iRGD magnetic targeting microbubble contrast agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The preparation method of the PLGA microbubble targeted by the magnetic iRGD comprises the following steps:

[0046] S1. Synthesis of DSPE-PEG 2000 -IRGD: Put the free tumor-penetrating peptide IRGD and DSPE-PEG-Maleimide in ultrapure water at a molar ratio of 1.5:1, in an ice bath environment (4°C), using a rotor (200r / min) Stir the reaction for 24 hours, then place the reaction solution in a dialysis bag (MWCO: 3500) and dialyze for 48 hours to filter out impurities, and finally transfer the dialyzed reaction solution to a vial with a pipette gun and place it in a -80°C refrigerator , after freezing, place the vial in freeze-drying for 48 hours to finally synthesize DSPE-PEG-iRGD, freeze-dry and store in a -20°C refrigerator for future use.

[0047] S2. Preparation of iron ferric oxide nanoparticle storage solution: first, ferric iron tetroxide nanoparticles are dispersed in double distilled water to form a suspension with a concentration of 2 mg / ml, and emulsified f...

Embodiment 2

[0050] Same as Example 1, the difference of Example 2 is that 1.5 mg of distearoylphosphatidylcholine and 1 mg of DSPE-PEG2000-iRGD are added, and other parameters and preparation methods are the same as in Example 1.

Embodiment 3

[0052] Same as Example 1, the difference of Example 3 is that 2 mg distearoylphosphatidylcholine and 0.5 mg DSPE-PEG2000-iRGD are added, and other parameters and preparation methods are the same as Example 1.

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Abstract

The invention relates to the technical field of biology, and particularly discloses an iRGD magnetic targeting microbubble contrast agent and application thereof. The iRGD magnetic targeting microbubble contrast agent comprises magnetic iRGD targeting PLGA microbubbles and ferroferric oxide nanoparticles entrapped on the surfaces of the PLGA microbubbles. According to the iRGD magnetic targeting microbubble contrast agent and the application thereof, iRGD and the ferroferric oxide nanoparticles are loaded on the PLGA microbubbles together, the effect of targeting new capillaries through the PLGA microbubbles is further improved, ultrasonic / magnetic resonance bimodal imaging is achieved, and the process of endometrial angiogenesis change is observed advantageously.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an iRGD magnetically targeted microbubble ultrasound / magnetic resonance dual-mode contrast agent and an application thereof. Background technique [0002] In vitro fertilization-embryo transfer (IVF-ET) is a common and effective alternative reproductive technology to help women become pregnant. However, due to insufficient endometrial receptivity, more than half of the transferred embryos fail to implant in the endometrium, even if they are normal. Endometrial receptivity, also known as the "window" for embryo implantation, refers to the state in which the endometrium is structurally and functionally ready to accept embryo implantation into the endometrium. Typically, this is a spatiotemporally limited planting window. This phase begins 6-8 days after the peak of luteinizing hormone and lasts about 48 hours in humans, the metasecretory and / or metaluteal phase, and is marked by the ...

Claims

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Application Information

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IPC IPC(8): A61K49/22A61K49/18A61K49/14A61K49/12A61K9/50A61K47/34A61K47/18
CPCA61K49/223A61K49/18A61K49/12A61K49/0002A61K9/5031A61K9/5015
Inventor 何燕妮刘红梅周美君龚哲立
Owner GUANGDONG NO 2 PROVINCIAL PEOPLES HOSPITAL
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