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Preparation method of ganoderan nano-selenium, ganoderan nano-selenium and application thereof

A technology of Ganoderma lucidum polysaccharide and nano-selenium, applied in nanotechnology, nanotechnology, nanomedicine, etc., can solve problems such as lack of research results, and achieve the effects of good storage stability, good pH tolerance and high safety

Inactive Publication Date: 2021-03-26
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is a lack of products combining Ganoderma lucidum polysaccharides and SeNPs in the anti-cancer field on the market, and there is also a lack of research results in this area. Therefore, research in this field has broad prospects

Method used

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  • Preparation method of ganoderan nano-selenium, ganoderan nano-selenium and application thereof
  • Preparation method of ganoderan nano-selenium, ganoderan nano-selenium and application thereof
  • Preparation method of ganoderan nano-selenium, ganoderan nano-selenium and application thereof

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Experimental program
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Effect test

Embodiment 1

[0047] 1) Preparation of Ganoderma lucidum polysaccharide nano-selenium solution

[0048]Sodium selenite was prepared into a 50mM solution with first-grade water, ascorbic acid was prepared into a 20mM solution, and Ganoderma lucidum polysaccharide was prepared into a 1mg / mL solution. Mix the sodium selenite solution evenly, add the volume to 10mL with first-grade water, then add 10mL of ascorbic acid solution dropwise, so that the final concentration of Ganoderma lucidum polysaccharide is 25, 50, 100, 200, 300 or 400 μg / ml, and the mixed solution After stirring and reacting for 12 hours at 30°C in the dark, the solution was transferred to an 8000Da dialysis bag, and dialyzed at 4°C in the dark for 72 hours to remove excess ascorbic acid and sodium selenite, and the particle size was measured using a nano-selenium particle size analyzer. The results are as follows figure 1 shown. from figure 1 It can be seen that, compared with the nano-selenium without Ganoderma lucidum pol...

Embodiment 2

[0054] 1) Stability evaluation of Ganoderma lucidum polysaccharide nano-selenium

[0055] The prepared nano-selenium polysaccharides of Ganoderma lucidum were stored at 4°C for 12 weeks, and then stored at different temperatures (4, 25, and 37°C) for 4 weeks. diameter, calculate the average value, the result is as Figure 12 , 13 shown. from Figure 12 It can be seen that the nano-selenium particle size of Ganoderma lucidum polysaccharides has no significant change after being placed at 4°C for 12 weeks, and the storage stability is high; from Figure 13 It can be seen that during the experimental storage period of 4 weeks, the nano-selenium particle size of Ganoderma lucidum polysaccharides placed at 4 °C did not change significantly, while the particle size of Ganoderma lucidum polysaccharide nano-selenium at 25 and 37 °C increased significantly to varying degrees. Large, and the largest change is at 37°C.

[0056] Use hydrochloric acid and sodium hydroxide solution to ...

Embodiment 3

[0058] 1) Toxicity of Ganoderma lucidum polysaccharide nano-selenium to cells

[0059] Mouse liver cancer cell Hepa1c1c7, mouse fibroblast NIH3T3, human liver cancer cell HepG2, human normal liver cell LO2 (5×10 3 per well) inoculated in Corning 96-well plate, at 37°C, 5% (CO 2 Concentration) of CO 2 Cultivate in the incubator for 24 hours; discard the old culture medium, add different concentrations of Ganoderma lucidum polysaccharide nano-selenium (2, 4, 8, 16, 32 μM), add fresh medium to the blank control group, and cultivate for 72 hours; take out the 96-well plate, discard Add 100 μL of serum-free medium containing 0.25 mg / mL MTT (thiazolium blue) to the old culture medium, at 37°C, 5% (CO 2 Concentration) of CO 2 Incubate in the incubator for 2 hours; take out the 96-well plate, discard the MTT (thiazolium blue), add 200 μL of dimethyl sulfoxide, put it in a shaker at 37°C at a speed of 100 rpm / min for 15 minutes, and then use a microplate reader to measure the 550nm ...

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Abstract

The invention discloses a preparation method of ganoderan nano-selenium, the ganoderan nano-selenium and application thereof. The preparation method comprises the steps that 1 a sodium selenite solution and a ganoderan solution are mixed to be uniform, ascorbic acid is dropwise added, a constant-temperature stirring reaction is conducted, and a reaction solution is obtained; and (2) the reaction solution obtained in the step (1) is dialyzed to obtain a ganoderan nano-selenium solution. Sodium selenite is used as a selenium source, ascorbic acid is used as a reducing agent, ganoderan is used asa surface modifier, a surface-functionalized nano-selenium compound is prepared in a green mode, and the ganoderan nano-selenium prepared through the preparation process has the advantages of being small in particle size, good in dispersity, amorphous, low in toxicity and good in stability, and can be used as a medicine for preventing and / or treating cancers.

Description

technical field [0001] The invention relates to a health care product and a processing method thereof, in particular to a preparation method of ganoderma polysaccharide nano-selenium, ganoderma polysaccharide nano-selenium and applications thereof. Background technique [0002] Worldwide, the morbidity and mortality of cancer have been increasing. According to statistics, there were 18.1 million new cases and 9.6 million deaths in 2018. Although humans have made a lot of efforts in the treatment of cancer, but The mortality rate has not decreased substantially, so scientists have shifted more attention to drug prevention of cancer. Studies in recent years have found that the application of nanomaterials as drugs has a positive effect on cancer prevention. [0003] Selenium is one of the essential micronutrients for the human body. Reasonable intake of selenium plays an important role in maintaining health and preventing diseases. Studies have shown that selenium entering t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61K33/04A61K36/074A61P35/00B82Y5/00
CPCC08B37/00A61K31/715A61K33/04A61P35/00A61K36/074B82Y5/00
Inventor 高雄吴清平陈少丹谢意珍李向敏陈地灵肖春胡惠萍潘鸿辉郑星
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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