Efficient straw enzymolysis method

Abstract

The invention belongs to the technical field of biology, and particularly relates to an efficient straw enzymolysis method. The efficient straw enzymolysis method comprises the following steps of (1)pretreatment of straws, namely, crushing and sieving the straws, putting the straws into a steamer, steaming with water vapor, adding a mixed solution of potassium hydrogen persulfate and boric acid,keeping warm, standing, adding into deionized water, keeping warm, standing, drying in a drying oven until the weight is constant, and performing high-pressure steam sterilization treatment to obtainpretreated straws; and (2) composite enzymolysis culture, namely, putting the pretreated straws into a culture medium containing trichoderma reesei and rhizopus oryzae, and performing culturing to obtain the product. According to the efficient straw enzymolysis method, the problem that the traditional single cellulase is low in enzymolysis effect is solved, the accessibility is further improved through a pretreatment method, the straw is fully utilized, the enzyme activity is enhanced through a culture medium containing hydrolyzed fish protein powder and cottonseed protein, and therefore, themethod has relatively high economic value.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for efficiently enzymolyzing straw. Background technique [0002] Straw is the general term for the stems and leaves (ears) of mature crops, including the remaining parts of wheat, rice, corn, potatoes, rapeseed and other crops after harvesting seeds. In an era of low agricultural production, straw was used for gaskets, to feed livestock, and for composting. [0003] Straw is composed of three major components: cellulose, hemicellulose and lignin. The cellulose is in the sheath of lignin, and lignin is a high-molecular polymer that is highly resistant to biodegradation and has a certain crystallinity. The higher the degree of polymerization and crystallinity, the more serious the encapsulation of lignin, which makes the biodegradation of cellulose more difficult. Experiments have proved that the enzymatic hydrolysis rate of straw without pretreatment is only abo...

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Problems solved by technology

[0004] The use of modern fertilizers has greatly reduced the demand for fertilizers made from straw in agriculture, and the disposal of straw has become a major problem. If straw is not disposed of in time, it will also affect the production of wheat, etc. In the sowing of autumn sowing crops, in order to deal with straw, large-scale burning of straw often occurs and has been repeatedly prohibited. While polluting air quality, it has caused great economic losses

[0005] The recycling of straw provides a new idea to solve this problem. At this stage, in-depth research has been carried out at home and abroad, and some achievements have been made, but it is still difficult to achieve large-scale Large-scale industrial production, saccharification and fermentation is one of the more mature methods in straw treatment. However, good results have not been achieved in terms of pretreatment methods and enzymatic hydrolysis conditions, especially in the case of stable and reliable application in industrial production.

[0006] Chinese patent application CN104561129A discloses a method for enzymatic hydrolysis and saccharification of straw. Will affect the final enzymatic hydrolysis effect

[0007] Chinese patent CN109182418B discloses a method for microbial enzymolysis and saccharification of straw, which can achieve a more effective enzymatic saccharification effect, but the fermentation medium used is too complicated and the cost is relatively high High, the straw after pretreatment has not been sterilized, and there is a risk of producing miscellaneous bacteria

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