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Picoliter-scale liquid injection device for cell injection, and injection method

An injection device and cell technology, applied in the field of cell injection, can solve the problems of low injection precision and complex structure

Pending Publication Date: 2021-03-19
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to provide a picoliter liquid injection device and injection method for cell injection, which solves the problems of low injection accuracy and complex structure in the current field of cell injection

Method used

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  • Picoliter-scale liquid injection device for cell injection, and injection method
  • Picoliter-scale liquid injection device for cell injection, and injection method
  • Picoliter-scale liquid injection device for cell injection, and injection method

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Embodiment Construction

[0036] The present invention will be further described in detail below in conjunction with specific embodiments, which are explanations of the present invention rather than limitations.

[0037] Such as figure 1 As shown, the present invention discloses a picoliter liquid injection device for cell injection, which includes a cell injection module, a motion control module and a microscopic imaging module, and the cell injection module, motion control module and microscopic imaging module are integrated in a shock-absorbing On stage 14.

[0038] The cell injection module includes a capillary glass tube 1 , an injection syringe 2 , a push rod 3 , a push rod fixing member 4 and an injection translation platform 5 , which are used to realize precise microinjection of the capillary glass tube 1 . The capillary glass tube 1 is closely connected through the installation hole at the end of the injection syringe 2, and it is easy to replace. The injection syringe 2 is fixed on the syr...

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Abstract

The invention belongs to the technical field of cell injection, and particularly discloses a picoliter-scale liquid injection device for cell injection, and an injection method. The device comprises acell injection module, a motion control module and a microimaging module; the cell injection module comprises a capillary glass tube, an injection needle cylinder, a push rod, an injection displacement table and a needle cylinder fixing block; the motion control module comprises a two-dimensional motion platform and a three-dimensional motion platform; a glass slide for bearing cells to be injected is placed on the two-dimensional motion platform; the injection displacement table and the injection needle cylinder fixing block are connected to the three-dimensional motion platform to realize three-dimensional positioning of the capillary glass tube; the upper end of the injection needle cylinder is connected with the push rod; the lower end of the injection needle cylinder is connected with the capillary glass tube; a push rod fixing piece is used for clamping the push rod; a sliding block is connected with an upper computer; and the upper computer controls the push rod to move to accurately control picoliter-grade liquid injection. The injection device disclosed by the invention is high in injection precision, compact and simple in structure and low in cost.

Description

technical field [0001] The invention relates to the technical field of cell injection, in particular to a picoliter liquid injection device and an injection method for cell injection. Background technique [0002] Cell injection utilizes a very fine-tip glass injection needle to directly inject foreign substances into cultured cells or prokaryotic embryos, which is the most commonly used method for cell or early embryo manipulation. This method of cell injection requires high-precision injection instruments and operating equipment. Traditional cell injection is mainly performed manually by researchers. Due to the limitation of the operating hardware level and the different operating capabilities of the operators, the success rate of cell injection experiments is low and the repeatability is poor. With the development of bioengineering and the development of cell injection automation, relevant research institutions at home and abroad have successively launched a variety of a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/89C12M1/00
CPCC12N15/89C12M33/04
Inventor 常博宋龙刚周权
Owner SHAANXI UNIV OF SCI & TECH
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