Method for cultivating broad-spectrum bacterial leaf streak resistant rice, primer and expression cassette

Abstract

The invention discloses a method for cultivating broad-spectrum bacterial leaf streak resistant rice, a primer and an expression cassette. The method comprises the following step that an OsSULTR3;6 gene promoter region is edited, so target sequences from the 422th site to the 403rd site on the upstream of a transcription start site of the OsSULTR3;6 gene are mutated to obtain the broad-spectrum bacterial leaf streak resistant rice; and the target sequences are nucleotide sequences as shown in SEQ ID No. 1. The provided method proposes constructing the bacterial leaf streak resistant rice through gene editing and breeding for the first time. According to the method, the OsSULTR3;6 gene promoter region is edited based on a CRISPR / Cas9 system; the obtained disease-resistant rice has no transgenic marker, the cultured new variety is similar to a natural mutation result, and the method can be widely applied to resistance breeding of various susceptible rice, is not limited by rice varieties, directionally changes rice characteristics, and is short in period and high in controllability.

Description

technical field [0001] The invention relates to a method for cultivating rice with broad-spectrum resistance to bacterial stripe disease, in particular to a method for cultivating rice with broad-spectrum resistance to bacterial stripe disease, a primer and an expression box. Background technique [0002] Rice bacterial stripe disease caused by Xanthomonas oryzae pv. oryzicola (hereinafter referred to as Xoc) is one of the important bacterial diseases that endanger rice production. The disease generally occurs in rice fields south of the Qinling Mountains and Huaihe River in my country, and can cause 40%-60% rice yield reduction when it is more severe. It is currently a quarantine disease in my country. At present, agricultural production mainly relies on pesticides to prevent and control the disease, but the use of pesticides can only temporarily reduce the harm of bacterial streak disease, and the cost is high, the pollution is heavy, and the safety is low. Germs will ine...

AI Technical Summary

Problems solved by technology

Some studies have shown that the pathogenicity of Xoc mainly depends on the overexpression of OsSULTR3; It is difficult to judge that the main virulence factor of these Xoc virulence strains is Tal2g

So far, there is no report on the use of this technology to edit the promoter region of the rice OsSULTR3; 6 gene to construct disease-resistant rice

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